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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium]
    #26843852 -

@phrontist I do like my half strength agar, same recipe but with half the ME (5g) and 1-2g Dextrose if you have it.


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My Magnum Opus:Thai Variety Comparison

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| Current Trade Print Availability:Some PESA and RW available for South African posters. Snail Mail only. |

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ManifoldPrime]
    #26843950 -



Something about this plate:

1) T3, completely unrelated to any other samples/sources
2) Looks what I would consider "weak" but
3) it seems to be colonizing the same rate horizontally.

Is this normal?  It's also interesting because I can still see light cycle rings and didn't expect it.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: w00tmycelium]
    #26844031 -

w00tmycelium said:


I'm sorry yall I'm having to take segments this large out because they're not sticking to the scalpel.  Better tooling maybe someday.



Try cutting small triangles, make sure you overcut so the triangle is completely free. . . Stab the triangle from the middle of one edge with just the tiniest tip of your scalpel at a downward angle of 30° or so. . .

Lift out and place on next plate. . .


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The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

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The Official Kiwi DMT and MAOI Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ManifoldPrime]
    #26844178 -

ManifoldPrime said:
@phrontist I do like my half strength agar, same recipe but with half the ME (5g) and 1-2g Dextrose if you have it.



Thanks MP. I’ll put that in my notes in case I have any dextrose and check it out. :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phrontist]
    #26845785 -

So, I made agar for the first time yesterday. There are small bits of brownish material floating around in it, but the consistency seems about right. You guys think they’re okay? Did I need to heat it longer? Higher heat?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phrontist]
    #26845794 -

If you used lme then it should be ok. If you don’t boil/microwave it enough before the pc there’ll be sediment.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26845818 -


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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #26845822 -

Are you using a recipe specifically for rhizo d3?


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26845838 -

Not necessarily, just sticking with what works good for me. 20g agar, 14g lme 1000ml distilled water

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26845840 -

Cool you’ve got a lot of rhizo plates so I was wondering

Have you run that culture yet? I hope it’s a giant white Melmak with rust spores and ape potency.


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LAGM2020

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26845851 -

It’s on grain right now but looks like maybe a tad too dry grain batch. Everything going looks like it’s stalling. Sucks cause it’s a good 20 jars or so of various strains

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #26845882 -

A.k.a said:
If you used lme then it should be ok. If you don’t boil/microwave it enough before the pc there’ll be sediment.



Cool, thanks for the tip.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phrontist]
    #26845966 -

I have some barley extract that tends to leave some brown strands In the agar. Doesn't seem to affect it at all.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Doyledozo]
    #26845974 -

I was gifted that barley when my father gave up doing gormets. Scored a huge roll of parafilm, some agar and nutritive yeast. Have yet to use any yeast. Is it even worth adding? Typically I just use LME.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc] * 1
    #26845992 -

D3monic said:






That's amazing


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Only hope can give rise to the emotion we call despair. But it is nearly impossible for a man to try to live without hope, so I guess that leaves Man no choice but to walk around with despair as his companion.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ichugwindex]
    #26846007 -

Lol, thanks!

AKA this is what I’m talking about with magenta plates growing weird. Think I’m going to toss it and only use blue, amber and charcoal for now on



Gotta be something in the dye

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26846656 -

Hey fellas-- do you think this Cuban plate is ready for dropping to rye, or do you see anything fishy about it.
It's a T4--


That 2-3 o'clock sector seems to be breaking out strong... but I'm not sure with the overall tomentose growth that it's ready for grain.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: coAsTal]
    #26846679 -

I’d be more worried about the bald patch followed by a wall at 6. Looks like it climbed over something

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Re: AGAR ENVY! (Anything and All things agar!) [Re: D3_Myc]
    #26846690 -

I have been working hard to get a clean plate-- but I've been plagued with very faint white mold/bacterial contam from this print that has blended in with the mycelium, so I've tried to pluck out sections away from it. I just can't seem to get it clean.

What do you think about that outer 2-3 section? Maybe a transfer from there to another tray.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: coAsTal]
    #26846742 -

9, 11 and 3 is where I’d grab from

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