Mr. Alien said:
I have a 0.8% nutes/500 ml LME broth that has been colonizing around 19-20 days since inoculation.

You guys think is ready or it needs more time?



EDIT:

Asura said:

I like it when it looks like a horse blew a load in the jar

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Mr. Alien said:

I have a 0.8% nutes/500 ml LME broth that has been colonizing around 19-20 days since inoculation.

You guys think is ready or it needs more time?

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I'd test on agar/of cake. The culture shouldn't be all settled at the bottom(lazy) and the broth looks all cloudy.

mushboy said:

Mr. Alien said:

I have a 0.8% nutes/500 ml LME broth that has been colonizing around 19-20 days since inoculation.

You guys think is ready or it needs more time?

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I'd test on agar/of cake. The culture shouldn't be all settled at the bottom(lazy) and the broth looks all cloudy.

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mushboy said:

I'd test on agar/of cake. The culture shouldn't be all settled at the bottom(lazy) and the broth looks all cloudy.

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Shr00merN00ber said:

Hey guys, looking to go for my first try at lc. Will dumping an agar wedge of clean growth (say 1cm x 1cm) in 250ml of sterilized lme water work? Cheers

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You can use microscopic fragments of myc if you want.

Expansion is fun

verum subsequentis said:

"LC test on agar"




Those pics were probably at two days. I can't remember. I know that i can usually be fairly certain of cleanliness/ filthiness  at 2 or 3 days. Apparently i don't have any pictures of test plates a little farther along. This is surprising. Oh well.

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