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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Agar questions
    #26045380 -

I made an agar tek with BRF and I thought I had messed up a lot. It was really hard to measure the ingredients in grams since it was a very small amount. I also made a mess by spilling agar in my microwave and then tried to put the stuff back.
Then I used a very very small part of a fresh mushroom just below the cap and put in the petri dish after microwaving while it was still liquid.

However, it all seems to have turned out good. It seems agar work is quite forgiving.

This is how it looks: https://imgur.com/a/2mIFYJ1

There are couple of things I wonder.

1 - Does it seem to be ready to use?
2 - For how long can I keep it?
3 - How should I apply it to sterilized rye? Should I microwave the whole petri dish and then pour it in a container with rye? Will that not kill the mycelium?

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Re: Agar questions [Re: Shroombie2]
    #26045394 -

oh my, Its very hard to follow you.I dunno wtf you did you get to that point but your dish does look "ok"
Is it ready to use? sure, i would transfer a rice sized piece from the edge and make a more uniform plate.
You can keep it for a few month in the fridge if it is wrapped properly.Longer if it was a bit thicker.
Do not microwave your myc. If you want to transfer that to grain.In a still air box you need something sharp and stainless steel that you can flame sterilize, cut into the dish and transfer into sterilized grain.


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2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

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Re: Agar questions [Re: bw86]
    #26045417 -

Thanks.

By making a more uniform plate, do you mean I should sterilize more agar with it's ingredients and then transfer from colonized agar to fresh agar?

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Re: Agar questions [Re: Shroombie2]
    #26045436 -

Yes, that's what he means. Although, I can't see the clone piece in your agar dish. There doesn't seem to be a point of origin for your growth.


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First Grow

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Re: Agar questions [Re: Raccoon]
    #26045455 -

there are several actually. One at top right and one near my thumb for example. I used very small pieces, almost as small as dirt beneath fingernails.

Here is a picture from the other side of the plate. https://imgur.com/a/C21G6qf

How come?

Edited by Shroombie2 (06/11/19 10:31 AM)

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Re: Agar questions [Re: Shroombie2]
    #26045461 -

I have three more questions.

1 - Should I have put it in the fridge directly after putting the mushroom in it or should I do it after colonization?

2 - Can I use a rice sized piece on sterilized rye as well? If so, Wow! I have a lot to colonize with already :laugh:!

3 - Should I be able to follow the same recipe but instead of a piece of mushroom use a spore syringe?

Edited by Shroombie2 (06/11/19 11:14 AM)

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Re: Agar questions [Re: Shroombie2] * 1
    #26045471 -

1. you put it in a fridge when you want to have backup culture aka storage. it grows and is kept on normal room temperatures in all other cases.

2. yes but will take ages to colonize. you will benefit from more than one inoculation point. dont put that myc on grains tho, plate does not look good.

3. yes but use PC and do it properly.


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Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond

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Re: Agar questions [Re: Shroombie2]
    #26045479 -

dont double post edit your last post if no one responds

1- you can put it in the fridge after it finishes colonizing
2-you can use a rice size piece of myc to colonize a grain jar but i would not recommend it. Maybe cut the dish so you can use 1/4 or 1/8
3- i dont know what recipe was used in the first place but you should be able to use the same recipe for both spores and tissue.


--------------------
2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

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Re: Agar questions [Re: Green_Hands]
    #26045483 -

Thanks,

Why doesn't it look good? It's mostly white on top. On the bottom, it's a bit yellowish but mostly white. I PCed it properly but then I got some hair shaft in it.
Does it need to be completely white everywhere?

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Re: Agar questions [Re: Shroombie2]
    #26045487 -

usually you want uniform rhizomorphic growth.Not splotchy with lots of different sectors.


--------------------
2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

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Re: Agar questions [Re: bw86]
    #26045503 -

I see! Thanks. That's why you suggested to make more agar from my agar. I will do that and then use that to inoculate grain. And then I will take pieces of rice sizes and inoculate grain from different ports.

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Re: Agar questions [Re: Shroombie2]
    #26045504 -

to me it looks a bit bacterial, yellow on plate is not good sign and growth is all over the place.

when i clone i go at least one/two transfers from original tissue, even if clean. that way you can be sure its clean - growth is more uniform, organized and easier to spot contaminants. bonus is you have few dishes to play with.
when you get your agar down you can start playing with LI and LC... that way one plate can inoculate hundreds of jars in no time.

agar is easy but not THAT easy. :smile: you are doing good, don't stop you are almost there.


--------------------


Looping our reflections, our obsessions draw us in
Fix and fixation, no sentience beyond

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Re: Agar questions [Re: Green_Hands]
    #26045865 -

Thanks for the tips. I will try to create LC/LI too.

I'm sterilizing new agar with BRF right now to inoculate with spore syringe and realized I have another question.

Last time, after sterilization, I let the agar cool and then microwaved it later to make it liquid. When it was liquid I put the bit of mushroom in it. I'm thinking now, after microwaving, the agar is quite hot while it's still liquid. Will the heat not kill the spores in the syringe? Can I not inoculate the agar when it's cool and solid?

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Re: Agar questions [Re: Shroombie2]
    #26045891 -

Yes that is what you are supposed to do. Somehow your mushroom tissue lived despite what you did last time.


--------------------
2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

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Re: Agar questions [Re: bw86]
    #26045925 -

Hmm, okay. Is there any reason to why I would microwave it after sterilization then?

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Re: Agar questions [Re: Shroombie2]
    #26046031 -

Oh, I read on Bod's agar BRF tek again. He microwaves it in order to be able to solve the ingredients in the water before sterilizing it. I didn't do that part now. I hope it turns out good anyway.

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Re: Agar questions [Re: Shroombie2]
    #26046091 -

Okay, I opened the lid of the flask too soon and the agar blew up and I completely burned my arm. Also, now I have a gelatin mess in my kitchen. Do you have any tips on how to clean my kitchen? :smile:

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Re: Agar questions [Re: Shroombie2]
    #26046106 -


DO NOT double or triple post edit your last post if no one responds
ask your mom for help


--------------------
2023 edible project & species list

Agar to grain/g2g = unlimited mushroomsAMU★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ :coffee:

Edited by bw86 (06/11/19 04:58 PM)

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Re: Agar questions [Re: bw86]
    #26046139 -

Okay. Go to hell. Or die. Who cares?
No need to reply if you are going to be a dick. I'm not that much in need of your help.

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Shop: Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

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