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OK plate for grains?
    #25935175 -

I want to put this Burma plate to grains, just wanted to check if it looks OK to anybody here.
This is a T2 from a print I took off of one of my first PF tek grows, streaked with a homemade loop. So much to learn I think my girlfriend is getting sick of me lol.

Thanks!


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Re: OK plate for grains? [Re: Dispsiple] * 1
    #25935183 -

Looks good, get those jars rocking:cheers:


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Re: OK plate for grains? [Re: Marmie]
    #25935234 -

sweet thank you for the prompt reply!


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Re: OK plate for grains? [Re: Dispsiple]
    #25958775 -

Got these guys into some whole Brown rice, colonized nicely IMO, but here's some pics of the jars.




And now one of the boxes, hoping this goes well!




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Edited by Dispsiple (04/27/19 12:27 PM)

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Re: OK plate for grains? [Re: Dispsiple] * 1
    #25958882 -

Looks awesome. Good luck.


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Yep.

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Re: OK plate for grains? [Re: Alchemycologist] * 1
    #25958909 -

Love the rings on that plate. Almost like a tree trunk section. Wonder wat causes that

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Re: OK plate for grains? [Re: thisbliss]
    #25958914 -

It looks good but there are 2 strains there. I would isolate them


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: OK plate for grains? [Re: Professor X]
    #25958971 -

I think I see what you are referring to, the area between 6 and 9 looks like it's growing at a different pace than the rest of the plate. Already too late too further isolate this plate though cut it up and dumped it into two separate jars of whole brown rice


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Re: OK plate for grains? [Re: thisbliss] * 1
    #25959109 -

No need to isolate at this point IMO, better off fruiting and taking clones. Looks nice and clean.

thisbliss said:
Love the rings on that plate. Almost like a tree trunk section. Wonder wat causes that




Could be temp swings from night to day, could be hidden bacteria


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Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
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Edited by AyePlus (04/27/19 08:14 PM)

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Re: OK plate for grains? [Re: AyePlus] * 3
    #25959237 -

the jars look lice ice cream


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Re: OK plate for grains? [Re: JHOVA]
    #25959522 -

Yeah you’re right,  those brown rice jars are lookin a bit bacterial. Agar looks pretty good, but those rings might be from hidden bacteria and not temp swings.

What was your grain prep?


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Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: OK plate for grains? [Re: AyePlus]
    #25959573 -

I pc'd at 15psi for about 110 min, vented for 10 min before putting the weight on top


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Re: OK plate for grains? [Re: Dispsiple]
    #25960155 -

Something happened: your nice culture went bacterial


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Re: OK plate for grains? [Re: JHOVA]
    #25960260 -

Welcome to my world, I've been striking out lately too lol.
I thought these plates would be winners but they were bacterial as hell.


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Edited by Nobler Hino (04/28/19 10:42 AM)

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Re: OK plate for grains? [Re: AyePlus]
    #25960517 -

Could bacteria be affecting myc in that regular intervals? How to detect hidden bacteria in agar is still a mistery for me, I don't know how you guys can do it from pics hehe

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Re: OK plate for grains? [Re: josevzs]
    #25960576 -

If you take a flashlight and shine through the bottom of the plate and see little spots that look like moisture but don't move when you tilt the plate, that's bacteria.


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My no pour Petri TEK - https://www.shroomery.org/forums/showflat.php/Number/27252059/page/1

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Re: OK plate for grains? [Re: Professor X]
    #25960604 -

Bacteria can also come from bad technique; not flaming scalpel enough, touching lip of jar with scalpel handle. All minor mistakes but if your grain is over hydrated any amount of bacteria will expand exponentially, especially when you shake the jars/bags.

josevzs said:
Could bacteria be affecting myc in that regular intervals? How to detect hidden bacteria in agar is still a mistery for me, I don't know how you guys can do it from pics hehe



Basically anything acting out of the ordinary is either enmeshed mold or bacteria. Comes with experience knowing what it should and shouldn’t looks like. Bacteria is microscopic but it causes visible changes in the organization of the myc.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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Re: OK plate for grains? [Re: AyePlus]
    #25961605 -

This is very helpful thank you! I think the rice may have been a little wet to begin with, and my technique is definitely lacking, but I am working on it. I appreciate the feedback, I'm willing to bet you are right about the rings in the plate being related to bacteria too looking at the tufts on the plate. I remember reading in a post that these can be a sign of bacteria riding along as well, you can see them more at the 3oclock area on the middle rings


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Re: OK plate for grains? [Re: Dispsiple]
    #25976593 -

First flush from the two boxes i made with this plate. They're definitely skinnier than the Golden teacher I have going right now but a much more even flush.
Larger box yielded 304g wet, 223g wet for the smaller box.

Much thanks to those who have helped me along the way I really appreciate the guidance!






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Edited by Dispsiple (05/06/19 08:55 PM)

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Re: OK plate for grains? [Re: Dispsiple]
    #25976699 -

Came out Great!

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