|
Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
|
No pour Petri dishes Professor X style TEK 4
#27252059 - 03/14/21 03:49 AM (2 years, 10 months ago) |
|
|
I've had a few people ask about the dishes I use so I'm making this TEK. Hopefully this helps some people get into the wonderful world of agar. You won't go far without doing so and the sky is the limit once you get the hang of it.
First things first, here are the plates and the link to purchase them

https://www.amazon.com/Augshy-Storage-Containers-Slime-Pack/dp/B0795CHXPR/ref=mp_s_a_1_3?dchild=1&keywords=augshy+2oz+slime+containers&qid=1615713671&sprefix=agushy+2oz&sr=8-3
You will also notice in the photo there is an exacto knife and a roll of 3M micropore tape. We will be needing these as well.
Step 1. Poke a tiny hole in both sides of each container directly beneath where the lid snaps on

Step 2. Tear a small piece of tape and cover each hole
That's it for the creation, now let's make agar and load the PC.
I make my agar with 1000ml Tap water, 25g LME and 25g telephone brand agar agar from the local Asian market. (This is enough agar for approximately 80 of these plates) Add LME to agar and shake in a small container or bag. Add to water cold and mix thoroughly. I add food coloring at this point. Bring to a boil stirring often. Watch closely while boiling especially in a small pot, it gets there quick and can easily boil over.
Once it is boiling pour into an easy pour measuring cup and fill the plates. Once you are finished snap on the lids, I pour just enough to cover the bottom of each plate.
PC Instructions.
Step 1 - fill 10 plates halfway with water and line the bottom of the pressure cooker, make sure the canning rack is in the bottom beneath the plates.

Step 2, place 2nd canning rack on top of plates full of water and fill PC with 2 quarts of hot water
Step 3, Load plates in PC in layers of 10, I place foil between each layer to prevent warping.
Step 4, PC @ 15psi for 45 minutes. You can go longer if you have contamination issues and I have gone up to 1.5 hours with no issue but don't personally feel the need for longer than 45 minutes. Let PC cool overnight and remove cold. I have let the dishes sit in the PC for days before and they are fine.
Step 5 (optional) condensation is normal with these plates and will clear up if left at room temperature for a week or so but if you want to speed the process stack them up and set in front of a ceramic space heater overnight.
Optional for very small batches - I do 4 in a presto rice pot. Place plates in top basket after filling pot with water. Use steam setting. Since the steam setting only does 99 minutes let it get to temp (about 30 minutes) stop and start again on 99 minutes. This has worked for me more than once when I have a new phenotype pop up I want a quick clone of without running a full load.

And for some of the results. Here are a couple T1 transfers I took from a Koh Samui Super Strain Albino clone last Friday!


I have used some of these plates a dozen times and they are still fine. They feel flimsy but they really hold up, the lids snap on and off easily as well which is detrimental to sterile technique.
Thanks for reading and if you like it give me some mush love!
Feel free to ask questions, I will answer any that I am able
Edited by Professor X (03/15/21 04:34 PM)
|
PeterPumpkinEater
Stranger

Registered: 08/25/19
Posts: 171
Last seen: 30 days, 11 hours
|
Re: No pour Petri dishes Professor X style TEK [Re: Professor X] 1
#27252062 - 03/14/21 04:09 AM (2 years, 10 months ago) |
|
|
Man this is awesome to see. I recently (like four weeks ago) also switched to autoclaveable PP5 autoclaveable plastic little minitubs.
However unlike you, I pour the agar (I use 15 grams agar, 10 grams malt extract, 400-500ml water, not a perfect recipe but unlike lower ratios is working out fine), and then wait about 24-48 hours for any spores to germinate (contams).
Then, I pop them, yes, in the microwave for 3 minutes (the lid is slightly ajar so they won't pop open). As you open the microwave you quickly squeeze them shut, its flamingly scalding hot and you will burn your fingers, but it lends a sense of assurance that no germinate spore could survive such temperatures, even were they presumably "thermophilic."
Believe me it works a charm and I just flame the needle and then the red hot needle melts a hole in the lid, and when I remove the needle - as I remove it I put a tiny blob of "bostik" ( a type of cheap superglue) on the hole. I've only had about 2 tubs contam, I just noticed one today, the lid was slightly ajar. The others are not contammed, I mean...
I did a bunch of PF teks with these tiny sized tubs too.
The magic for me is I don't have to pressure cook it, the microwave really does do the job. Its far less of a bother than the pressure cooker. To hell with petri dishes I say and long live pp5 plastic minitubs, you can make tiny little PF-cakes in them, too. My bathroom is encrusted with spores and mold, a testament to the perfection of an absurd technique in the form of sterile cultures. :p
Editor's note:
When they cool off I put them in my Still air box, which was bleach cleaned and has moist bleach cloths ( a bleach-water soaked tshirt on the bottom) - (I cut two warm pants' leggings off which I use as ruffled arm-jackets ("gloves") to close the holes, also moist with bleach-water to catch any flying particles. I have a tiny ozone generator with a little UV C bulb attached to it, and you can just smell when its done its job in the still air box. I put my hot tubs under a bleach soaked cloth in this still air box so when they cool down they don't suck in contams. inoculate the next day. So its pour agar / make PF-tek mix. Wait a day or two. Microwave. Possibly microwave again for 3 minutes, put in still air box with its little ozone generator. inoculate a day later or so. Sorry for the rant I'm drinking redwine its noon on Sunday and I'm alone, so :p
My protocol(s) seems to work quite remarkably actually. Again for me the "magical ability" to let your agar or mini PF cakes in your pp5 plastic tubs sit for a day or two (a little neglect goes a long way) to let contams and other endospores germinate makes them so prone for the microwave's vicious effects.
-------------------- How Dare I? =================== Donations: ================= 1LWaph7vqgZVDJgYMKc2nmeSqA5xjxYUrY =================== Donations: =================
Edited by PeterPumpkinEater (03/14/21 04:20 AM)
|
alaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
|
|
Not usually a fan of plastic but I got a 50 pack of these recently too and was far more pleased than I thought I was going to be with them. Not only did they survive the PC fine, but also have great functionality as far as lid function is concerned. I was excited to see you mention in another thread they held up well. I had virtually no condensation and can use them within like an hour of PCing (of course, like all my prepours, I let them come back to room temperature after pouring/before lidding, and remove from PC hot- 2 things that have virtually eliminated prepour condensation). Nice writeup bud.
-------------------- "First we build the tools, then they build us." THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities
|
PeterPumpkinEater
Stranger

Registered: 08/25/19
Posts: 171
Last seen: 30 days, 11 hours
|
|
Quote:
alaskappalachian said: Not usually a fan of plastic but I got a 50 pack of these recently too and was far more pleased than I thought I was going to be with them. Not only did they survive the PC fine, but also have great functionality as far as lid function is concerned. I was excited to see you mention in another thread they held up well. I had virtually no condensation and can use them within like an hour of PCing (of course, like all my prepours, I let them come back to room temperature after pouring/before lidding, and remove from PC hot- 2 things that have virtually eliminated prepour condensation). Nice writeup bud.
Try the microwave. 3 minutes x 2. I do another 2 minutes on a follow up day. just wait a day or so (not sure about ambient temps) prior to the first microwaving as you'd want endospores to have exposed themselves via germination.
I also found the plastic doesn't give you that sense of scientific authority as an official petri dish but on a practical level its opened new horizons for me using pp5 plastic and a microwave. I actually thought about getting a t-shirt that says "I <3 plastic" but of course people would be offended not realising its ubiquitous and ambiguous role in sustaining their life whilst simulatenously destroying the purity of your sushi at the local yuppie-bar.
I want to add taking care to notice the lids are properly snapped shut after microwaving (for me) is a key problem - but its more about my conscientiousness rather than the apparently flimsy yet effective nature of the snappy lids. It reflects an oversight where one to have been in such a hurry to not have noticed.
Edited by PeterPumpkinEater (03/14/21 04:50 AM)
|
Benson
The Kidd ⭐


Registered: 09/29/20
Posts: 837
|
|
Hell yeah, thanks for the writeup professor. I appreciate you including how you stack them in the PC as well, I've always had trouble stacking pasty plates and such without having them fall over mid-cycle. Definitely going to give this a try, fuck filling landfills with single use plastic 
-------------------- [||||||||||||||||||||||||||||||||||||] [||||||||||||||||||||||||||||||||||||] [||||||||||||||||||||||||||||||||||||]
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
|
Re: No pour Petri dishes Professor X style TEK [Re: Benson]
#27252748 - 03/14/21 04:21 PM (2 years, 10 months ago) |
|
|
i have these slime contsiners as well but i just pour with them, great price on them and a good size
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
MycFunk3D
Stranger
Registered: 10/07/20
Posts: 9
Last seen: 2 years, 10 months
|
Re: No pour Petri dishes Professor X style TEK [Re: cronicr]
#27252755 - 03/14/21 04:32 PM (2 years, 10 months ago) |
|
|
Bookmark
|
Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
|
Re: No pour Petri dishes Professor X style TEK [Re: cronicr]
#27252813 - 03/14/21 05:23 PM (2 years, 10 months ago) |
|
|
Quote:
cronicr said: i have these slime contsiners as well but i just pour with them, great price on them and a good size
I have thought about doing this as well as I have a flowhood but I like the flexibility no pours provide, I just pour them, snap the lid on, Load, fire up the good ole stove and watch TV with the wife til they're done.
|
Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
|
Re: No pour Petri dishes Professor X style TEK [Re: Professor X]
#27252819 - 03/14/21 05:33 PM (2 years, 10 months ago) |
|
|
Using the same containers and they are great, I have also tried to pour them a few times which works well but no pour suit me better, reserving the pour for real petris.
|
Veggiesandhemp
Strainger

Registered: 12/08/20
Posts: 77
Loc: Cascadia
Last seen: 1 year, 2 months
|
Re: No pour Petri dishes Professor X style TEK [Re: Professor X]
#27252820 - 03/14/21 05:33 PM (2 years, 10 months ago) |
|
|
Sweet tek! Ty!
|
Mycelium Juice
Here for the weed



Registered: 10/31/20
Posts: 505
Loc: planet earth
|
Re: No pour Petri dishes Professor X style TEK *DELETED* *DELETED* [Re: Professor X]
#27253256 - 03/15/21 12:43 AM (2 years, 10 months ago) |
|
|
Post deleted by Mycelium Juice
Reason for deletion: 🏴☠️
|
Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
|
Re: No pour Petri dishes Professor X style TEK [Re: Mycelium Juice]
#27253267 - 03/15/21 01:29 AM (2 years, 10 months ago) |
|
|
Quote:
Mycelium Juice said: Nice write-up X. Simple, to the point, and super easy to execute. Much appreciated!
No problem at all. Glad you enjoyed it.
|
verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 7 months
|
Re: No pour Petri dishes Professor X style TEK [Re: Professor X]
#27253291 - 03/15/21 02:14 AM (2 years, 10 months ago) |
|
|
Nice write up.
Quote:
I place foil between each layer to prevent warping.
What's the logic here?
Only Thing I'd suggest adding is the proverbial "watch closely while boiling" line. Just cause most noobs don't realize how quickly it'll boil over.
|
Baba Yaga
♥ coir grower

Registered: 09/13/20
Posts: 3,955
Loc: Hyperspace Chicken Coop
|
|
I think what he means is the the inner part of the bottom on these cup is raised and when you stag them there is a void between the bottom of the upper cup and the lid of the lower one where a vacuum can form causing deformation of the lid, at least that is what happened to mine when I didn't put a separation layer in between.
|
Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
|
Re: No pour Petri dishes Professor X style TEK [Re: Baba Yaga]
#27253465 - 03/15/21 06:50 AM (2 years, 10 months ago) |
|
|
Quote:
Baba Yaga said: I think what he means is the the inner part of the bottom on these cup is raised and when you stag them there is a void between the bottom of the upper cup and the lid of the lower one where a vacuum can form causing deformation of the lid, at least that is what happened to mine when I didn't put a separation layer in between.
100% this, tried stacking without one time. Never did it again.
|
Professor X
School for the Gifted



Registered: 04/18/19
Posts: 2,719
Last seen: 2 years, 3 months
|
|
Quote:
verum subsequentis said: Nice write up.
Quote:
I place foil between each layer to prevent warping.
What's the logic here?
Only Thing I'd suggest adding is the proverbial "watch closely while boiling" line. Just cause most noobs don't realize how quickly it'll boil over.
You're absolutely right. Suggestion taken and line added. Thank you. Having dome this so long I forget about these things sometimes but now that you say something about it I remember walking out of the room to grab my phone because it was ringing. I came back to a huge mess and was only gone maybe 10 to 15 seconds.
|
Nimpo
Big Black


Registered: 05/10/12
Posts: 2,375
|
Re: No pour Petri dishes Professor X style TEK [Re: Professor X]
#27254000 - 03/15/21 01:41 PM (2 years, 10 months ago) |
|
|
Hey Professor, Im seeing some reviews on these plates talking about how fragile they are and how easy the lid can break when opening them.
Have you encountered any of these issues yourself? (or anyone else who has bought them).
|
smalltalk_canceled
Babnik


Registered: 07/13/20
Posts: 2,862
Last seen: 11 days, 16 hours
|
Re: No pour Petri dishes Professor X style TEK [Re: Nimpo]
#27254006 - 03/15/21 01:48 PM (2 years, 10 months ago) |
|
|
Good job professor
-------------------- Willpower is the one true virtue
  
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
|
Re: No pour Petri dishes Professor X style TEK [Re: Nimpo]
#27254008 - 03/15/21 01:51 PM (2 years, 10 months ago) |
|
|
Quote:
Nimpo said: Hey Professor, Im seeing some reviews on these plates talking about how fragile they are and how easy the lid can break when opening them.
Have you encountered any of these issues yourself? (or anyone else who has bought them).
yes they can be fragile but they are cheap so its expected lol
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Nimpo
Big Black


Registered: 05/10/12
Posts: 2,375
|
Re: No pour Petri dishes Professor X style TEK [Re: cronicr]
#27254018 - 03/15/21 01:59 PM (2 years, 10 months ago) |
|
|
Quote:
cronicr said:
Quote:
Nimpo said: Hey Professor, Im seeing some reviews on these plates talking about how fragile they are and how easy the lid can break when opening them.
Have you encountered any of these issues yourself? (or anyone else who has bought them).
yes they can be fragile but they are cheap so its expected lol
Bahh 
Im a clumsy fucker and can see many broken plates in my future. Ill grab two orders to offset the ones I know Ill be breaking.
|
|