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Offlinedeathblade
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A question on cloning?
    #11207381 - 10/08/09 01:59 PM (8 years, 2 months ago)

If I were to take a clone that is from a shroom grown on sheep manure would that clones offspring be more susceptible to growing on sheep manure?


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InvisibleShea25
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Re: A question on cloning? [Re: deathblade]
    #11207810 - 10/08/09 03:08 PM (8 years, 2 months ago)

No It would be a clone of that mushroom but would grow off most nutritional substrate like, cow poo, horse poo, BRF+Verm, ect... the same as long as you provide it with optimal conditions


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Offlinedeathblade
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Re: A question on cloning? [Re: Shea25]
    #11208076 - 10/08/09 03:52 PM (8 years, 2 months ago)

ok. I was just curious.


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Offlinedeathblade
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Re: A question on cloning? [Re: deathblade]
    #11230024 - 10/12/09 12:24 AM (8 years, 2 months ago)

I prepared the petri dishes and today I put a few little chunks onto them. The technique I used was to pick a couple of shrooms that werent fully grown and then put them into my glove box. I ripped them in half and picked a small chunk from the inside of the stem and put it onto the agar. Will this work? Or is there a different method to be used when dealing with P.Cube?


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InvisibleDoc_T
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Re: A question on cloning? [Re: deathblade]
    #11231624 - 10/12/09 08:43 AM (8 years, 2 months ago)

That's it. You should start seeing something soon.


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OfflineHybridprX
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Re: A question on cloning? [Re: Doc_T]
    #11232006 - 10/12/09 11:30 AM (8 years, 2 months ago)

This is more of a topic for advanced mycology.

I'm not a fungal geneticist so I won't pretend to be but from my understanding while researching agar additives I read somewhere that mycelium can go into a state of shock from being removed from a fresh patch, that is why spores are collected from the fruit bodies and printed prior to starting fresh cultures for indoor cultivation.

I honestly don't know how much of this I believe myself but that is what I gather If someone more experienced in the geneticist department of fungi knows better please elaborate the air.

If I were hardcore enough to go out and collect wild specimen's I would personally start from spores and clean them up on agar prior to indoor cultivation of the species, It makes sense that a outdoor patch transplanted indoors would more then likely contaminate unless you took some of the fungi itself and cleaned it on agar...


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OfflineMigraine
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Re: A question on cloning? [Re: HybridprX]
    #11232670 - 10/12/09 01:49 PM (8 years, 2 months ago)

A better place to take the sample from would be the in the cap - just above were the gills are developed.

This tends to be the newest and most active area for cell growth.

Makes sure you make a hole in the agar and place the tissue sample in so that 75% of the sample is in the agar hole.  This gives the best surface area contact so that the mycelium can jump from tissue to agar.

Goor Luck.


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