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OfflineBurninJ
Stranger

Registered: 05/11/03
Posts: 11
Last seen: 12 years, 4 months
no dikaryon in clone = no friuts?
    #1539750 - 05/11/03 09:29 PM (15 years, 1 month ago)

Hey shroomers, this is my first post here!
one quick question/problem:
in order to continue a cake that fruited well for future generations I used some mycelium from the centre of the cake to clone it to a culutre jar that i then used to inocculate several other cakes. But now when I tried to fruit these cakes they refuse to or produce deformed fruits! :frown:
Is it because i happened to use monokaryotic mycelium in the cloning, instead of dikayotic? Should i  have used a fruiting body for cloning instead?
Any ideas, what did i do wrong? Thanks in advance to anyone who replies!     


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OfflineLegoulash
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Registered: 09/07/02
Posts: 4,347
Last seen: 7 years, 4 days
Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1539759 - 05/11/03 09:34 PM (15 years, 1 month ago)

Big words.. Bahumbug.. I dont think u can clone from mycelium.. I think its more like picking out teh strongest most agresive mycelium..But im no expert.


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Invisiblepoke smot!
floccinocci floofinator
Male

Registered: 01/08/03
Posts: 5,235
Re: no dikaryon in clone = no friuts? *DELETED* [Re: Legoulash]
    #1539792 - 05/11/03 10:01 PM (15 years, 1 month ago)

Post deleted by poke smot!

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OfflineBurninJ
Stranger

Registered: 05/11/03
Posts: 11
Last seen: 12 years, 4 months
Re: no dikaryon in clone = no friuts? [Re: poke smot!]
    #1539934 - 05/11/03 11:05 PM (15 years, 1 month ago)

Thanks magic and poke.
Poke smot, I've figured that much out, what i'm asking is it possible that i used mycelium that was monokaryotic despite using mycelium from a fruiting cake?
Should u always use a fruit to clone?


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OfflineFunkyBudah
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Registered: 04/25/03
Posts: 430
Loc: Shakedown Street
Last seen: 3 months, 23 days
Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1540425 - 05/12/03 01:33 AM (15 years, 1 month ago)

I believe that you did a transfer, not a clone.


-FB


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Invisiblewoodrow
journeyman
Registered: 03/17/03
Posts: 138
Re: no dikaryon in clone = no friuts? [Re: FunkyBudah]
    #1540890 - 05/12/03 04:56 AM (15 years, 1 month ago)

It is possible to transfer from mycelium but if the mycelium smaple was too small (like less than a quarter inch chunk) it could have been monokaryotic. If the growth from the mycelium was rhizomorphic or sectored (it should look like the edge of a feather) then the sample was probably not monokaryotic and you have other problems. Monokaryotic growth is tometose (looks like cotton) and does not produce fruits.


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Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 3 years, 8 months
Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1541533 - 05/12/03 01:26 PM (15 years, 1 month ago)

if you had a cake of good mycelium, then you automaticly had dikaritotic cells and they never go back to monokarions.

your problem is a manifestation of senesence or contamination. this question belongs in the advanced forum. good question


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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