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InvisibleShea25
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How to sterilize a syringe * 14
    #12098357 - 02/25/10 10:47 PM (11 years, 5 months ago)

How to sterilize a syringe with just a pot water and syringe 

Thing needed.

A pot
Empty Syringe
Water(tap can work)


Fill the pot with cold water. turn the burner on High and wait for the water to come to a full boil. now start a 10 minute countdown and keep the water at a full boil.

After 10 minutes take the empty syringe and suck back the boiling water and let it sit  in the syringe for 1 minute then expel the water in a sink(not back in the pot)

Repeat 4 more times on the last time keep the water in the syringe cap it and let it cool down.

Hope this helps.







x4







Notes:
Use a Q-tip and 70% alcohol to clean the inside of the cap
I have found even after months and months they will stay sterile
No need to store in the fridge
Never try to cool them down fast in the fridge


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Offlinersinatra13
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Re: How to sterilize a syringe [Re: Shea25]
    #12098598 - 02/25/10 11:22 PM (11 years, 5 months ago)

wow who knew it could be so simple!
(i did :awedance:)



haha. great tek shea. although its simple, i feel like it was needed. so thank you
:thumbup:


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Invisiblebare.whiterabbit
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Re: How to sterilize a syringe [Re: Shea25]
    #12098604 - 02/25/10 11:22 PM (11 years, 5 months ago)

My shit, thank you very much Shea...I was going to the lengths of filling the syringes with water and putting everything in a jar and PCing

This will make life much easier, in the future...not that I didn't know to use alc in the cap :uhoh:


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InvisibleSmartweed
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Re: How to sterilize a syringe [Re: Shea25]
    #12098615 - 02/25/10 11:24 PM (11 years, 5 months ago)

Do you leave an air bubble in the syringe so when you suction up the spores you will be able to shake em up later if they clump up. How do I make sure that air bubble doesnt have any type of contam.


--------------------
I believe that if people would learn to use LSD's vision-inducing capability more wisely, under suitable conditions, in medical practice and in conjunction with meditation, then in the future this problem child could become a wonderchild. - HOFFMAN

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Invisiblehamloaf
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Re: How to sterilize a syringe [Re: Smartweed]
    #12098620 - 02/25/10 11:25 PM (11 years, 5 months ago)

Do you think that you would be able to do the same thing only with the syringes drawn to sterlize the air inside the syringe for, Gas Exchanging Liquid Cultures. "sterile air syringes, SAS. I totally just made that last part up. I am a dork. I know.


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OfflineRandomstickynote
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Re: How to sterilize a syringe [Re: Shea25]
    #12098627 - 02/25/10 11:25 PM (11 years, 5 months ago)

Quote:

Shea25 said:
Never try to cool them down fast in the fridge



What does that do, like whats the harm? I always just let mine sit a few hours, but it seems you could just throw it in a fresh ziploc and stick that in the fridge if you were in a hurry?


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InvisibleShea25
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Re: How to sterilize a syringe [Re: Smartweed]
    #12098628 - 02/25/10 11:26 PM (11 years, 5 months ago)

After you make the spore syringe hold the needle into a flame till red hot and suck back a few bubbles of air


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InvisibleShea25
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Re: How to sterilize a syringe [Re: Randomstickynote]
    #12098655 - 02/25/10 11:30 PM (11 years, 5 months ago)

Quote:

Randomstickynote said:
Quote:

Shea25 said:
Never try to cool them down fast in the fridge



What does that do, like whats the harm? I always just let mine sit a few hours, but it seems you could just throw it in a fresh ziploc and stick that in the fridge if you were in a hurry?




I'm not a fan of rapid expansion and contraction of plastics.


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OfflineRandomstickynote
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Re: How to sterilize a syringe [Re: Shea25]
    #12098669 - 02/25/10 11:32 PM (11 years, 5 months ago)

Quote:

Shea25 said:
Quote:

Randomstickynote said:
Quote:

Shea25 said:
Never try to cool them down fast in the fridge



What does that do, like whats the harm? I always just let mine sit a few hours, but it seems you could just throw it in a fresh ziploc and stick that in the fridge if you were in a hurry?




I'm not a fan of rapid expansion and contraction of plastics.



So the chemicals from the plastics will be going into your spore syringes? Making them sterile, or potentially allowing toxins into your substrate? I'm not understanding the harm, I apologize.


--------------------
AMU


Edited by Randomstickynote (02/25/10 11:44 PM)


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InvisibleSmartweed
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Re: How to sterilize a syringe [Re: Randomstickynote]
    #12098717 - 02/25/10 11:40 PM (11 years, 5 months ago)

Quote:

Randomstickynote said:
So the chemicals from the plastics will be going into your spore syringes? Making them sterile, or potentially allowing toxins into your substrate? I'm not understanding the harm, I apologize.




There will be no chemicals going in the syringe, your just sterilizing them.


--------------------
I believe that if people would learn to use LSD's vision-inducing capability more wisely, under suitable conditions, in medical practice and in conjunction with meditation, then in the future this problem child could become a wonderchild. - HOFFMAN

My Print/Syringe Trade List


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InvisibleShea25
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Re: How to sterilize a syringe [Re: Randomstickynote]
    #12098730 - 02/25/10 11:42 PM (11 years, 5 months ago)

Quote:

Randomstickynote said:
So the chemicals from the plastics will be going into your spore syringes? Making them sterile, or potentially allowing toxins into your substrate? I'm not understanding the harm, I apologize.




Its harder on the syringes


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OfflineRandomstickynote
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Re: How to sterilize a syringe [Re: Shea25]
    #12098743 - 02/25/10 11:44 PM (11 years, 5 months ago)

Quote:

Smartweed said:
Quote:

Randomstickynote said:
So the chemicals from the plastics will be going into your spore syringes? Making them sterile, or potentially allowing toxins into your substrate? I'm not understanding the harm, I apologize.




There will be no chemicals going in the syringe, your just sterilizing them.



I'm refering to shea's post in that one, not the act of sterilizing a syringe.

Oh and hey, an alternative way to breaking up spore clumps, without sucking air bubbles in or shaking is to set it on a subwoofer or speaker, the vibrations will do the job.

Quote:

Shea25 said:
Its harder on the syringes




That makes sense. Okydokie.


--------------------
AMU


Edited by Randomstickynote (02/25/10 11:46 PM)


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InvisibleShea25
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Re: How to sterilize a syringe [Re: Randomstickynote]
    #12098751 - 02/25/10 11:46 PM (11 years, 5 months ago)

I have herd that vibrations and temp changes can cause spores to germinate, making the syringes illegal


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OfflineRandomstickynote
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Re: How to sterilize a syringe [Re: Shea25]
    #12098764 - 02/25/10 11:48 PM (11 years, 5 months ago)

Quote:

Shea25 said:
I have herd that vibrations and temp changes can cause spores to germinate, making the syringes illegal



:shocked:


--------------------
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Invisiblewhyblameus
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Re: How to sterilize a syringe [Re: Shea25]
    #12098840 - 02/26/10 12:02 AM (11 years, 5 months ago)

Quote:

Shea25 said:
I have herd that vibrations and temp changes can cause spores to germinate, making the syringes illegal



i have had lots of spore syringes germinate just keeping them at room temp after making them.

so i highly suggest keeping you spore syringes in the fridge after making them if you plan on sending them.


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InvisibleShea25
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Re: How to sterilize a syringe [Re: whyblameus]
    #12098850 - 02/26/10 12:03 AM (11 years, 5 months ago)

Yea always store syringe in the fridge


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InvisibleShea25
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Re: How to sterilize a syringe [Re: whyblameus]
    #12098851 - 02/26/10 12:04 AM (11 years, 5 months ago)

Quote:

whyblameus said:
Quote:

Shea25 said:
I have herd that vibrations and temp changes can cause spores to germinate, making the syringes illegal



i have had lots of spore syringes germinate just keeping them at room temp after making them.


[gradient:#FF3D03,#FF3D03]so i highly suggest keeping you spore syringes in the fridge after making them if you plan on sending them.[/gradient]





Are you using tap water>?


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Invisiblewhyblameus
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Re: How to sterilize a syringe [Re: Shea25]
    #12098888 - 02/26/10 12:08 AM (11 years, 5 months ago)

no i use distilled water.

but i do it different.

i just put it in a half pint jar and microwave it till it boils.

let it cool then suck into syringes.(that i sterilized in the pc for 15minutes)

im gonna start pcing my water like i do with lc that will probable help.:shrug:


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InvisibleShea25
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Re: How to sterilize a syringe [Re: whyblameus]
    #12098897 - 02/26/10 12:09 AM (11 years, 5 months ago)

Lol maybe the nuked water is no good


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Invisiblewhyblameus
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Re: How to sterilize a syringe [Re: Shea25]
    #12098907 - 02/26/10 12:12 AM (11 years, 5 months ago)

:shrug:

it spore solution was fine.
no contam just germination.
i didnt mind i wasnt sending them to anyone so it wasnt a problem.
i was just confused when it happened.:lol:


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