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OfflineUnclePimpy
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HoneyTek and Tissue Cloning questions...
    #546591 - 02/10/02 02:19 PM (15 years, 10 months ago)

Wasabi,

Couple of quick questions:

1) I recently innoculated a jar using the HoneyTek and Puerto Rican cub. strain. It's been over 52 hrs and there are no changes....has anyone had real success with this tek?

2)When cloning mycelium into other substrate jars, is it absolutely necessary to put the mycelium slices into H2O2 and then draw them into a syringe? If there were sterile enough conditions, couldn't one potentially just place the slices directly into the substrate?

Thanks in advance!



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Offlinefresh357
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Re: HoneyTek and Tissue Cloning questions... [Re: UnclePimpy]
    #546891 - 02/10/02 08:34 PM (15 years, 10 months ago)

1. Yes much success. You have to watch out for bacteria though. I add a lot of h2o2, keep at reasonably warm temp.

2 Hypothetically, but do you really want to take that risk? Is a little sterility precaution worth losing half (at least) your jars?


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InvisibleZen Peddler
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Re: HoneyTek and Tissue Cloning questions... [Re: UnclePimpy]
    #546978 - 02/10/02 09:31 PM (15 years, 10 months ago)

Ive never had much success using honey mediums - malt has always worked for me and im experimenting (as Lizardking suggests) with brf paste.
Id definately recommend using h202 as any precaution available always helps. You could avoid using a syringe by extracting the tissue and mycelia - placing it in a dilatuted h202 solution then adding it to a substrate. Or you could try adding a bit of h202 to the substrate as well for a precaution - there are no sproes to germinate, so h202 is invaliable for you!


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Amazon Shop: ½ Pint Jars, Brown Rice Flour

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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