Home | Community | Message Board


Kraken Kratom
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Brown Rice Flour

Jump to first unread post. Pages: 1
OfflineUnclePimpy
Stranger
Registered: 02/10/02
Posts: 17
Loc: Dirty Souf'
Last seen: 14 years, 11 months
HoneyTek and Tissue Cloning questions...
    #546591 - 02/10/02 02:19 PM (15 years, 16 days ago)

Wasabi,

Couple of quick questions:

1) I recently innoculated a jar using the HoneyTek and Puerto Rican cub. strain. It's been over 52 hrs and there are no changes....has anyone had real success with this tek?

2)When cloning mycelium into other substrate jars, is it absolutely necessary to put the mycelium slices into H2O2 and then draw them into a syringe? If there were sterile enough conditions, couldn't one potentially just place the slices directly into the substrate?

Thanks in advance!



--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinefresh357
addict

Registered: 09/02/01
Posts: 493
Last seen: 14 years, 10 months
Re: HoneyTek and Tissue Cloning questions... [Re: UnclePimpy]
    #546891 - 02/10/02 08:34 PM (15 years, 16 days ago)

1. Yes much success. You have to watch out for bacteria though. I add a lot of h2o2, keep at reasonably warm temp.

2 Hypothetically, but do you really want to take that risk? Is a little sterility precaution worth losing half (at least) your jars?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleZen Peddler
Male User Gallery

Registered: 06/18/01
Posts: 6,379
Loc: orbit
Re: HoneyTek and Tissue Cloning questions... [Re: UnclePimpy]
    #546978 - 02/10/02 09:31 PM (15 years, 16 days ago)

Ive never had much success using honey mediums - malt has always worked for me and im experimenting (as Lizardking suggests) with brf paste.
Id definately recommend using h202 as any precaution available always helps. You could avoid using a syringe by extracting the tissue and mycelia - placing it in a dilatuted h202 solution then adding it to a substrate. Or you could try adding a bit of h202 to the substrate as well for a precaution - there are no sproes to germinate, so h202 is invaliable for you!


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Brown Rice Flour

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* Tissue Sample question thefinalhours 511 9 03/02/09 08:06 PM
by goldieman
* Tissue cloning using the honey Tek(NEW PICS) jebus2000 2,249 12 09/23/01 03:10 PM
by felixhigh
* cloning question Callampin 855 8 01/06/08 04:49 AM
by Nibin
* agar again and clone questions
anakin3197
565 8 03/13/09 02:21 PM
by anakin3197
* Cloning question - saving a clone subject? *DELETED* Ophanim 713 6 12/17/07 09:57 AM
by FooMan
* cloning (shrooms not humans), need expert opinion. QuitDrinking 1,602 5 12/30/02 08:50 AM
by Curious_George
* Really quick cloning question peruvian spark 552 4 03/03/04 09:24 PM
by RogerRabbit
* Couple of LC clone questions? Cloneufc 661 3 02/24/09 02:34 PM
by Buckeye Oysters

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Magash, Shroomism, george castanza, Prisoner#1, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, EvilMushroom666, cronicr, PussyFart, Tmethyl
675 topic views. 41 members, 128 guests and 43 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Kraken Kratom
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.004 seconds on 14 queries.