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OfflineQuitDrinking
Stranger
Registered: 09/04/02
Posts: 52
Loc: SC
Last seen: 14 years, 9 months
cloning (shrooms not humans), need expert opinion.
    #1175392 - 12/28/02 03:06 PM (14 years, 9 months ago)

I had few problems with contams(lost a few jars out of 12) but I have noticed that my pinning in jars/fruiting is not as good as with the spore syringe I used last time. (First attempt at each) PF strain (Matias Romero)
Other variables that may ....
1. Temp varied from 60-90 during pinning stage (in jars). Mostly kept in 70-80 as called for though.( temp was more stable during spore syringe growth and fruiting)
2. Mushroom used was nearly mature rather than young and rapidly growing as called for. I am thinking that a not so fast growing shroom may have produced mycelium of a like mind.
Any thoughts?
On the plus side these are making better spore prints than the first batch.
I'll try a dunk and get back to you, with the results.


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.


Edited by QuitDrinking (12/30/02 08:30 AM)


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OfflineQuitDrinking
Stranger
Registered: 09/04/02
Posts: 52
Loc: SC
Last seen: 14 years, 9 months
Re: cloning question, need expert opinion. Or yours will do... [Re: QuitDrinking]
    #1179061 - 12/30/02 08:07 AM (14 years, 9 months ago)

Forgive me for bumping this up, but I would like to get some opinions on this


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 7 months
Re: cloning question, need expert opinion. Or yours will do... [Re: QuitDrinking]
    #1179070 - 12/30/02 08:13 AM (14 years, 9 months ago)

how big were the jars, and of what?? brf, millet, rye??

You fruited directly from the jar!! that works,, but why?

You cloned tissue from an existing shroom??? on agar? or did you throw the clone tissue directly in the jar?

Or.. are you talking spores from your first harvest??

confused??  :confused:

cg


--------------------
************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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OfflineQuitDrinking
Stranger
Registered: 09/04/02
Posts: 52
Loc: SC
Last seen: 14 years, 9 months
Re: cloning question, need expert opinion. Or yours will do... [Re: Curious_George]
    #1179085 - 12/30/02 08:18 AM (14 years, 9 months ago)

1/2 pint BRF, cloned tissue from shroom benderized with boiled and cooled water, allowed pins to form in jars as per PF Tek, then transfered to fruiting chamber


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineQuitDrinking
Stranger
Registered: 09/04/02
Posts: 52
Loc: SC
Last seen: 14 years, 9 months
Re: cloning (shrooms not humans), need expert opinion. [Re: QuitDrinking]
    #1179155 - 12/30/02 08:45 AM (14 years, 9 months ago)

If I had tits, you guys would have given me an answer by now. Well actually I DO have tits, but they are hairy.... you wouldn't want to see them


--------------------
Free the Rapist and Muderers!!!
We need more prison space for the Pot heads.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 7 months
Re: cloning (shrooms not humans), need expert opinion. [Re: QuitDrinking]
    #1179172 - 12/30/02 08:50 AM (14 years, 9 months ago)

You are right!!! maybe post some nice ones for inspiration!!!

Your question is waayyyy too vague!! hard to answer!!

cg


--------------------
************************************.>>>>>
Here Johny,, have a joint.. all your friends are doing it!!


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