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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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no dikaryon in clone = no friuts?
    #1539750 -

Hey shroomers, this is my first post here!
one quick question/problem:
in order to continue a cake that fruited well for future generations I used some mycelium from the centre of the cake to clone it to a culutre jar that i then used to inocculate several other cakes. But now when I tried to fruit these cakes they refuse to or produce deformed fruits! :frown:
Is it because i happened to use monokaryotic mycelium in the cloning, instead of dikayotic? Should i  have used a fruiting body for cloning instead?
Any ideas, what did i do wrong? Thanks in advance to anyone who replies!     

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Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1539759 -

Big words.. Bahumbug.. I dont think u can clone from mycelium.. I think its more like picking out teh strongest most agresive mycelium..But im no expert.

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Re: no dikaryon in clone = no friuts? *DELETED* [Re: Legoulash]
    #1539792 -

Post deleted by poke smot!

Reason for deletion: x


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Re: no dikaryon in clone = no friuts? [Re: poke smot!]
    #1539934 -

Thanks magic and poke.
Poke smot, I've figured that much out, what i'm asking is it possible that i used mycelium that was monokaryotic despite using mycelium from a fruiting cake?
Should u always use a fruit to clone?

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Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1540425 -

I believe that you did a transfer, not a clone.


-FB

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Re: no dikaryon in clone = no friuts? [Re: FunkyBudah]
    #1540890 -

It is possible to transfer from mycelium but if the mycelium smaple was too small (like less than a quarter inch chunk) it could have been monokaryotic. If the growth from the mycelium was rhizomorphic or sectored (it should look like the edge of a feather) then the sample was probably not monokaryotic and you have other problems. Monokaryotic growth is tometose (looks like cotton) and does not produce fruits.

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Re: no dikaryon in clone = no friuts? [Re: BurninJ]
    #1541533 -

if you had a cake of good mycelium, then you automaticly had dikaritotic cells and they never go back to monokarions.

your problem is a manifestation of senesence or contamination. this question belongs in the advanced forum. good question

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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