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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
cloning..... over and over and over again
    #1031411 - 11/07/02 06:07 AM (18 years, 8 months ago)

OK, if i clone the biggest mushroom from a flush, then grow it up and clone the biggest shroom from that flush and keep doing this for years, would I eventually end up with a seriously massive shroom? kind of like that mendel guy with the peas.


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Offlinemlv
Stranger
Registered: 07/06/02
Posts: 114
Last seen: 14 years, 2 months
Re: cloning..... over and over and over again [Re: Zildjian]
    #1031423 - 11/07/02 06:19 AM (18 years, 8 months ago)

have a look at this


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Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: cloning..... over and over and over again [Re: mlv]
    #1031430 - 11/07/02 06:25 AM (18 years, 8 months ago)

so what's better?
grow, print, grow, print, grow, print, grow print...
or grow, clone, grow, clone, grow, clone, grow, clone...


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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: cloning..... over and over and over again [Re: zeta]
    #1031448 - 11/07/02 06:36 AM (18 years, 8 months ago)

In reply to:


The reports you hear of continued cloning resulting in mutation are reports from commercial growers. The range of mutation and strain degradation is around 1000 generations before you start seeing it. Think about how long a home cultivator would take to run out 1000 generations. You are not likely to have a problem with strain degradation in your lifetime



to better isolate and 'evolve' or select a strain you would grow, print, clone, grow, print, clone, each time selecting better fruiters and colonizers ect


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_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


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Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: cloning..... over and over and over again [Re: Cow Shit Collector]
    #1031451 - 11/07/02 06:38 AM (18 years, 8 months ago)

you can't clone a print!


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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: cloning..... over and over and over again [Re: zeta]
    #1031457 - 11/07/02 06:43 AM (18 years, 8 months ago)

mlv, thanks for the link.


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OfflineAnnoA
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Registered: 06/18/99
Posts: 24,143
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Re: cloning..... over and over and over again [Re: Zildjian]
    #1031472 - 11/07/02 06:59 AM (18 years, 8 months ago)

> kind of like that mendel guy with the peas.

Mendel did selection after sexual propagation.
If you clone it all the time, you don?t have any recombination of genes by sexual means.


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Invisiblegeorge castanzaM
Lord Of The Idiots!
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Registered: 10/21/02
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Re: cloning..... over and over and over again [Re: mlv]
    #1031480 - 11/07/02 07:05 AM (18 years, 8 months ago)

that link rocks.thax  :wink: 


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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: cloning..... over and over and over again [Re: Anno]
    #1031499 - 11/07/02 07:27 AM (18 years, 8 months ago)

Anno, I apologise for my lack of knowledge about the asexual? reproduction of fungi, but i was wondering. . .
If i took clones of the biggest shrooms from two seperate prints and put them togeather on a agar plate, would any sort of genetic 'mixing' occur where their mycelia met?

Sorry for asking stupid questions, but my small brain has difficulty comprehending complex theories such as sexual reproduction, more so now since i intially started this post as I electricuited myself with 240V about 10 minutes ago.


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Invisiblegeorge castanzaM
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Re: cloning..... over and over and over again [Re: Zildjian]
    #1031506 - 11/07/02 07:33 AM (18 years, 8 months ago)

you shocked yourself....electricuition is death from electical shock



just being a smartass :tongue: 


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KRAMER CAKES




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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: cloning..... over and over and over again [Re: Zildjian]
    #1031509 - 11/07/02 07:35 AM (18 years, 8 months ago)

you could clone the best strain unless you felt you wanted to refine again in which case you'd go back to the print board (pun)


--------------------
_______________________________________
CSC


Life's a garden, Dig it!
~Joe Dirt

Off Topic Website


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InvisibleZildjian
human

Registered: 09/11/02
Posts: 208
Loc: new zealand
Re: cloning..... over and over and over again [Re: Cow Shit Collector]
    #1031517 - 11/07/02 07:41 AM (18 years, 8 months ago)

ok, i think i'll do the clone grow print grow clone grow thing, fucking hell that shock was scarey. I think i'm going to be scared of electricity for a while.


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Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: cloning..... over and over and over again [Re: george castanza]
    #1031528 - 11/07/02 07:49 AM (18 years, 8 months ago)

In reply to:

electricuition is death from electical shock



Wow.. I never knew that.
Electrocute = electro + execute


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Invisiblezeta
Stranger

Registered: 05/25/02
Posts: 3,972
Re: cloning..... over and over and over again [Re: Zildjian]
    #1031534 - 11/07/02 07:52 AM (18 years, 8 months ago)

In reply to:

If i took clones of the biggest shrooms from two seperate prints and put them togeather on a agar plate, would any sort of genetic 'mixing' occur




Spores germinate into monokaryons.. monokaryons mate to form dikaryons.
Mushroom tissue is already composed of dikaryons so no, you couldn't mate two clones.
The only way to mate is if you bring together individual monokaryons. This is pretty hard.. usually achieved by diluting spore solution many times and germinating on agar.


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OfflineLoop_Theorist
addict
Registered: 04/22/02
Posts: 604
Last seen: 14 years, 10 months
Re: cloning..... over and over and over again [Re: Zildjian]
    #1031747 - 11/07/02 10:00 AM (18 years, 8 months ago)

240v? you're lucky to be walking around my friend.


-Loop


--------------------
Pack-tight, midnight, ship it to tha coast.
Some take tha plane, you, you on tha boat,
right beside tha money which right beside tha coke
Passin by tha coastgaurd, right under their nose.

To the US-HEY where anyone can getny, MIA all the way to New Your city, you a twenty now....


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OfflineI_Fart_Blue
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Registered: 06/25/02
Posts: 3,495
Loc: SItting on the Group W Be...
Last seen: 17 years, 6 months
Re: cloning..... over and over and over again [Re: Loop_Theorist]
    #1032198 - 11/07/02 01:16 PM (18 years, 8 months ago)

Volts have nothing to do with the matter. I've been shocked by 10000 volts. I was more supprised than anything. It's the amps which will kill you.


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"A study of the history of opinion is a necessary preliminary to the emancipation of the mind. I do not know which makes a man more conservative-to know nothing but the present, or nothing but the past." -John Maynard Keynes


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Invisiblegeorge castanzaM
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Registered: 10/21/02
Posts: 8,096
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Re: cloning..... over and over and over again [Re: I_Fart_Blue]
    #1032531 - 11/07/02 03:05 PM (18 years, 8 months ago)

it's the amperage that kills you :wink:              volts x amps =watts 


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KRAMER CAKES




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