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 ilikeshrooms
newbie
Registered: 09/11/02
Posts: 46
Last seen: 14 years, 11 months
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 Myco Bag Incubation 
#905588 - 09/24/02 10:43 PM (15 years, 1 day ago) |
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Ok, my hamster has ordered 4 myco environment bags and a puerto rican spore syringe. He has a great place to incubate it, but he's wondering if it has enough humidity. He wants to store it in the room with the water heater where the temps. stay around 80 degrees all day so he won't overheat the bags. This place is warm, dark and unfortunately dry. Can he still use the room to incubate the myco bags? He also knows about the perlite humidification TEK so he's wondering if he can solve the humidity problem by placing the bags inside of an aquarium lined with perlite and placed inside the water heater room. He appreciates your help.
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 daussaulit
Forgetful
Registered: 08/06/02
Posts: 2,894
Loc: Earth
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my bags are at about 85 degrees, and they're doing great. If it's too dry, inject some sterilized water into the bag. I've had to do that many many times because where my bags are sitting, there is low humidity. I haven't had a contamination problem yet.
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ilikeshrooms
newbie
Registered: 09/11/02
Posts: 46
Last seen: 14 years, 11 months
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 Re: Myco Bag Incubation [Re: daussaulit]
#905616 - 09/24/02 10:59 PM (15 years, 1 day ago) |
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how would I go about getting sterilized water into a syringe?
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blink
eye of horus
Registered: 03/31/02
Posts: 11,343
Loc: Geographic Location (Stat...
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sterilize syringe full of water by boiling it inside a larger container of water (without the other container, the needle will arc , i did it by accident)
then suck up some boiled water if any evaporates out or whatever
or just PC it and then suck up water, but nuking it is faster
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Edited by blinkidiot (09/24/02 11:31 PM)
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ilikeshrooms
newbie
Registered: 09/11/02
Posts: 46
Last seen: 14 years, 11 months
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Re: Myco Bag Incubation [Re: blink]
#905738 - 09/25/02 12:01 AM (15 years, 1 day ago) |
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sterilize syringe full of water by boiling it inside a larger container of water (without the other container, the needle will arc , i did it by accident)
then suck up some boiled water if any evaporates out or whatever
or just PC it and then suck up water, but nuking it is faster
I'm a little lost here. I want to sterilize the syringe in a large container of water ie, a pot and then suck up some water with the same syringe?
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daussaulit
Forgetful
Registered: 08/06/02
Posts: 2,894
Loc: Earth
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I just nuked a bottle of water in the microwave, and capped it and let it cool. I then nuke the syringe(without the metal needle) in the microwave to kill the germs. I take the needle and dump it in some isopropyl alcohol. put the syringe together, uncap the bottle of water, suck up the water, cap the bottle, and then sterilize the needle and the injector spot with rubbing alcohol again, and then inject. I've had to inject at least 20 syringefuls of water, no contam problem at all.
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