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InvisiblePsiloSurfer
Ghost
Registered: 01/24/02
Posts: 20
Myco Bag - Incubation Problems
    #555678 - 02/18/02 10:13 PM (15 years, 5 months ago)

Okay, here is an overview of the procedure:

I recieved 1 EQ syringe and 4 Myco Environment Bags from Sporeworks on 2/08/01, and proceeded to innoculate the bags.

I took a shower, put on freshly laundered clothes, washed my hands with antibacterial soap, and strapped a dust mask to my face. I sterilized my workspace by spraying the walls/countertops with a 10% bleach solution, let it sit for 10 minutes, and wiped dry with a clean terrycloth.

I sterilized a spot on the outside of a myco bag with 70% isopropyl alcohol, sterilized the needle with 70% isopropyl alcohol, and inserted the needle into the 'air balloon' above the substrate. I injected a little over 2cc's spore solution, removed the needle, and immediately taped over the hole with scotch tape. I then mixed up the substrate and broke up all the chunks to distribute the the spore solution.

I repeated the process with the remaining bags.

I put the bags back in the box, and put the box in a warm and dark place. The temperature varies between 75-85 degrees f.

It has been 10 days. The bags look exactly like they did when i recieved them. No mycelium growth is apparent.

What could be the problem? Do you have any advice? Was there a flaw in my procedure?

Thanks. Any help would be appreciated.


Edited by PsiloSurfer (02/19/02 12:52 PM)


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OfflineSupershrooomer
journeyman
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Registered: 11/02/01
Posts: 112
Last seen: 7 years, 8 months
Re: Myco Bag - Incubation Problems [Re: PsiloSurfer]
    #555753 - 02/18/02 11:54 PM (15 years, 5 months ago)

I would give it a couple more days, then if you still see nothing contact sporeworks. They will probably hook you up.


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InvisibleSouthernGent
veteran
Registered: 01/12/02
Posts: 1,331
Re: Myco Bag - Incubation Problems [Re: PsiloSurfer]
    #555993 - 02/19/02 06:57 AM (15 years, 5 months ago)

You may need another syringe.


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 6 months, 17 days
Re: Myco Bag - Incubation Problems [Re: PsiloSurfer]
    #556100 - 02/19/02 11:11 AM (15 years, 5 months ago)

Did you gently shake the syringe a bit before you injected the liquid?


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Just another spore in the wind.


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InvisibleSouthernGent
veteran
Registered: 01/12/02
Posts: 1,331
Re: Myco Bag - Incubation Problems [Re: Seuss]
    #556109 - 02/19/02 11:23 AM (15 years, 5 months ago)

Are you supposed to shake the syringe?


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Offlineroykinn
enthusiast

Registered: 10/15/01
Posts: 247
Last seen: 15 years, 2 months
Re: Myco Bag - Incubation Problems [Re: SouthernGent]
    #556129 - 02/19/02 12:07 PM (15 years, 5 months ago)

Yes, it helps break up the chunks of spores that have stuck together to distribute them into the water more evenly-

roy


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"You can't be a real country unless you have a beer and an airline- It helps if you have some kind of a football team, or some nunclear weapons, but at the very least you need a beer." -Frank Zappa


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InvisiblePsiloSurfer
Ghost
Registered: 01/24/02
Posts: 20
Re: Myco Bag - Incubation Problems [Re: Seuss]
    #556156 - 02/19/02 12:50 PM (15 years, 5 months ago)

Yes, I did shake the syringe to distribute the spores evenly in the solution.


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InvisibleFreakyFreddy
newbie
Registered: 11/23/01
Posts: 35
Re: Myco Bag - Incubation Problems [Re: PsiloSurfer]
    #556246 - 02/19/02 02:42 PM (15 years, 5 months ago)

Its time to shake your bags.


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