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OfflineWildLASER
Infinity In AGrain Of Salt

Registered: 04/02/00
Posts: 870
Last seen: 2 years, 9 months
Re: How long can you sustain a strain . . . . .???
    #86234 - 06/10/00 05:35 PM (22 years, 3 months ago)

Doing it this way, as you know, the strain will defintely degrade. However, if you make a culture, using agar or, the simpler but less forgiving honey tek, you can keep that culture going forever. Just transfer a wedge of agar / few cc's of honey water/ mycelium solution, to a new culture jar/dish. The Honey Tek is under Grow/Find if you want it, this is the way to go IMHO, I am trying to do the honey tek right now, starting a new jar soon.


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Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86236 - 06/10/00 06:04 PM (22 years, 3 months ago)

So degradation of the strain occurs when starting from the previous generations spores every time?

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Trying to rationalize with an irrational person, is in itself irrational!



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Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86237 - 06/10/00 06:42 PM (22 years, 3 months ago)

no way! it is mycelium to mycelium transfers that result in strain degradation.

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He who controls the spice, controls the universe....



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Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86238 - 06/10/00 08:27 PM (22 years, 3 months ago)

Senescens does eventually occur when starting new generations from the spores of previous generations on the same substrate indefinitely. Senescens can be avoided with a few strategies. Culturing spores onto agar and isolating the dikaryotic growth is a good way to ensure a vigorous culture. The cultures can then be stored at 35-40 degrees for long periods of time. Every six months, the cultures should be transferred to an alternate agar formulation. Using this method meticulously, strains can be preserved indefinitely.

I would imagine that varying your substrate for successive generations might work nearly as well, but having a tissue bank in reserve is a safer bet.

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"I have wiped entire civilizations off of my chest with a grey gymsock!"

[This message has been edited by SpaceFarmer (edited June 10, 2000).]



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