Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineWildLASER
Infinity In AGrain Of Salt

Registered: 04/01/00
Posts: 870
Last seen: 4 years, 4 months
Re: How long can you sustain a strain . . . . .???
    #86234 - 06/10/00 08:35 AM (23 years, 10 months ago)

Doing it this way, as you know, the strain will defintely degrade. However, if you make a culture, using agar or, the simpler but less forgiving honey tek, you can keep that culture going forever. Just transfer a wedge of agar / few cc's of honey water/ mycelium solution, to a new culture jar/dish. The Honey Tek is under Grow/Find if you want it, this is the way to go IMHO, I am trying to do the honey tek right now, starting a new jar soon.


--------------------
,'~Þ

Extras: Filter Print Post Top
Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86236 - 06/10/00 09:04 AM (23 years, 10 months ago)

So degradation of the strain occurs when starting from the previous generations spores every time?

------------------
Trying to rationalize with an irrational person, is in itself irrational!


Extras: Filter Print Post Top
Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86237 - 06/10/00 09:42 AM (23 years, 10 months ago)

no way! it is mycelium to mycelium transfers that result in strain degradation.

------------------
He who controls the spice, controls the universe....


Extras: Filter Print Post Top
Anonymous

Re: How long can you sustain a strain . . . . .??? [Re: WildLASER]
    #86238 - 06/10/00 11:27 AM (23 years, 10 months ago)

Senescens does eventually occur when starting new generations from the spores of previous generations on the same substrate indefinitely. Senescens can be avoided with a few strategies. Culturing spores onto agar and isolating the dikaryotic growth is a good way to ensure a vigorous culture. The cultures can then be stored at 35-40 degrees for long periods of time. Every six months, the cultures should be transferred to an alternate agar formulation. Using this method meticulously, strains can be preserved indefinitely.

I would imagine that varying your substrate for successive generations might work nearly as well, but having a tissue bank in reserve is a safer bet.

------------------
"I have wiped entire civilizations off of my chest with a grey gymsock!"

[This message has been edited by SpaceFarmer (edited June 10, 2000).]


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Substrate Bags   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Post deleted by Moe Howard X.O 1,367 11 01/22/03 08:24 PM
by Anno
* Re: Mushroom Strain Improvement and Breeding? Ripper 4,896 10 02/10/00 07:01 AM
by Anonymous
* Getting a strain isolate without agar ??
( 1 2 3 4 all )
majik_monkey 14,023 61 01/13/04 10:44 PM
by Joshua
* Crossing two strains IS possible..Heres how...
( 1 2 3 4 5 all )
BlueJay 35,318 96 06/01/15 10:34 PM
by God25
* Agar Strain Isolation - Is it worth it?
( 1 2 all )
Blue Helix 13,107 20 02/23/05 01:07 AM
by Blue Helix
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 9,155 18 07/24/01 12:02 AM
by Anno
* grain transfers - maximum possible? bwo 1,716 4 06/17/02 09:13 AM
by durban_poison
* Re: can a non hallucinogenic edible mushroom strain be cross bred with a hallucinogenic? mycofile 3,279 8 12/15/99 08:52 PM
by aaron

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
1,139 topic views. 0 members, 8 guests and 0 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.006 seconds on 14 queries.