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Doing it this way, as you know, the strain will defintely degrade. However, if you make a culture, using agar or, the simpler but less forgiving honey tek, you can keep that culture going forever. Just transfer a wedge of agar / few cc's of honey water/ mycelium solution, to a new culture jar/dish. The Honey Tek is under Grow/Find if you want it, this is the way to go IMHO, I am trying to do the honey tek right now, starting a new jar soon.
Senescens does eventually occur when starting new generations from the spores of previous generations on the same substrate indefinitely. Senescens can be avoided with a few strategies. Culturing spores onto agar and isolating the dikaryotic growth is a good way to ensure a vigorous culture. The cultures can then be stored at 35-40 degrees for long periods of time. Every six months, the cultures should be transferred to an alternate agar formulation. Using this method meticulously, strains can be preserved indefinitely.
I would imagine that varying your substrate for successive generations might work nearly as well, but having a tissue bank in reserve is a safer bet.
------------------ "I have wiped entire civilizations off of my chest with a grey gymsock!"
[This message has been edited by SpaceFarmer (edited June 10, 2000).]