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InvisibleHippie3
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Re: Honey jar help
    #85556 - 05/12/00 09:05 PM (16 years, 6 months ago)

wait a few days longer.
it's been only 3 or so...
spore germination time will normally be 3-5 days so i'd be patient.
minor points.
you could use a tablespoon instead of teaspoon next time.
pre-heating the honey water in microwave and stirring to disolve helps reduce sediment.
using ice to speed cooling might have caused some suction of contam'd air into the jar.
pressure cook it as you would agar.

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Anonymous

Re: Honey jar help
    #85558 - 05/12/00 09:08 PM (16 years, 6 months ago)

Laser I used about 16 ounces of water and injected 4cc's worth of PF spores. It took a good week before I saw anything substancial. My best guess is to just give it some time. Good luck.


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OfflineWildLASER
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Re: Honey jar help
    #85559 - 05/12/00 10:25 PM (16 years, 6 months ago)

Thank you all for your help, you have given me some hope for my honey jar, I just arrived back from dinner, during which the honey jar was wrapped in a heating blanket that goes from Low-Hi with 9 in-between, had it on 4.


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OfflineWildLASER
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Re: Honey jar help
    #85560 - 05/14/00 10:42 PM (16 years, 6 months ago)

UPDATE: Been considerably more hours, I think about 4-5 days, no notcieable growth really, water is real cloudy and I think I see some mycelium on the bottom, as well as the black spots (spores I think) floating around (a few), I shake it alot. Is this ~ok for PFMR, I hope I get growth soon.


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OfflineWildLASER
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Re: Honey jar help
    #85561 - 05/15/00 06:00 PM (16 years, 6 months ago)

It has now been 6-7 days and all I see is some very cloudy white water, more so when shaken, a few bits of the spore stuff, and a bit of white shit that was in there (looks like a rock of perlite) that looks brownish now, still, no noticeable mycelium, but it does look like wispy shit and smoke when I shake the bottle, then it dissolves. Should I try making one substrate jar to see if this works or what? It is pissing me off that no growth has shown in 6 days or so using this (PFMR) strain.


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Anonymous

Re: Honey jar help
    #85562 - 05/16/00 11:51 AM (16 years, 6 months ago)

I too have had both sucess and failure with the honey water tec.

1st for the success: I took straight spring h20, 400ml, and added 1.5 rounded teaspoon of raw honey bought at the health store. This honey is unfiltered and has some fine sediment in it. pressure cook for 25 mins, innoculate with some of Ryche Hawk's ecuadorian strain spores. incubate at 84F for 3 days, massive mycelial growth.

now the failures: same technique only with cambodian and panaeolous trop. spores instead. Same smoky, fine, dusty yellow appearance in jars but no positive mycelium.
its been 2 weeks on the cambodians and a week on the pan trop. oh, teh pan trop is at 70-75F.

I dont know whether a change in parameters is called for or whether this smoky cloudy silt is actually germinated tissue. It doesnt seem to me like it is contamination.
any more thoughts wildlaser?



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InvisibleHippie3
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Re: Honey jar help
    #85563 - 05/16/00 11:56 AM (16 years, 6 months ago)

some are slow starters. i wouldn't pitch it just yet but starting another might be prudent.
bacteria might be present.

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Anonymous

Re: Honey jar help
    #85564 - 05/16/00 02:57 PM (16 years, 6 months ago)

I was wondering if anyone else has had success/failure w/Ryche's Cambodian strain and this honey tek...Thanks as always! :smile:


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OfflineWildLASER
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Re: Honey jar help
    #85565 - 05/16/00 08:54 PM (16 years, 6 months ago)

I noticed that smoky stuff now appears solidified on the bottom rather then just when I shake it, also, I do not have a pressuer cooker and boiled for about 35 min, is this sufficient?


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OfflineWildLASER
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Re: Honey jar help
    #85566 - 05/16/00 08:57 PM (16 years, 6 months ago)

On further investigation (checked jar again), it appears nothing is on the bottom (like the smokey stuff, it redistributes when shaken) I thin I am going to start a new jar, boil it for an hour, and let it cool overnight, then inoculate with 2cc spore water instead of 1.


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OfflineWildLASER
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Re: Honey jar help
    #85567 - 05/16/00 11:10 PM (16 years, 6 months ago)

I am leaving this jar for now, hoever I just started a new jar, had more honey this time and pre-disolved it stirring in the honey after heating spring water in microwave. It is currentely boiling, for about 1 hour total, and then I will incoulate with 2ccs spore water and put in my closet, maybe trying to set up a heating blanket this time.


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Offlineaural
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Re: Honey jar help
    #85568 - 05/17/00 01:18 AM (16 years, 6 months ago)

If you are looking at sediments at the bottom of a bottle of boiled honey water,I should mention that I brew mead,which is basically fermented boiled honey water.When you boil it,some proteins and stuff coagulate and stuff,then settle out to the bottom....


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Offlinebillthecat
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Re: Honey jar help
    #85569 - 05/17/00 10:49 PM (16 years, 6 months ago)

hey aural,
this doesn't really relate to the topic, but i was wondering if you could point me in the direction of some good literature on brewing mead...

thanks,
-bill the cat



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OfflineWildLASER
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Re: Honey jar help
    #85570 - 05/20/00 12:35 AM (16 years, 6 months ago)

This new jar doesn't look like it is making much progress, been 3-5 days, not sure, but I do not see much, maybe what looks like a few spores germinated and made some white stuff but then quit or something.


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OfflineWildLASER
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Re: Honey jar help
    #85571 - 05/20/00 02:07 PM (16 years, 6 months ago)

Thats it! I think my syringe was dead, I tried 2 honey jars and did everything perfectly, and double checked it all the second time, I boiled for an hour and 5 minutes the second time, and was more sterile, still NO GROWTH, I think I am going to ask for a new syringe.


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Anonymous

Re: Honey jar help
    #85572 - 05/21/00 12:25 PM (16 years, 6 months ago)

I am obviously not an expert, or anything close to it in this category. But could carmalization be a problem. You said you sterilized for 1 hour+, this is a bit mch I have heard half an hour.


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OfflineWildLASER
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Re: Honey jar help
    #85573 - 05/21/00 10:03 AM (16 years, 6 months ago)

I tried 30-35 the first time and got no growth as well.


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OfflineWildLASER
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Re: Honey jar help
    #85574 - 05/21/00 07:16 PM (16 years, 6 months ago)

I might be getting/borrowing a pressure cooker, as this will probably speed things up and increase my chances.


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OfflineWildLASER
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Re: Honey jar help
    #85575 - 05/21/00 09:00 PM (16 years, 6 months ago)

DAMN THAT STUNK!!! I just opened my first honey jar (second attempt is same I bet) and I was reminded of the chemistry rule of WAFTING and not sniffing! The slightly yellowish liquid had a pungent odor of bacteria, not a nice mushroomy scent, but the crap that you grow on broth exposed to the air if you have ever done that experiment. I do not know HOW it was contaminated, or how it beat the spores even germinating, but I suspect these spores are bad too. Any help? I mixed the honey and water kinda untersterily, sprayed everything down with lysol, I thought it wouldn't matter since I was sterilizing it anyway. This jar I boiled for 30 minutes, tape over the hole, I think contaminated air was sucked into the jar, how can I prevent this? When I puncture the hole, air rushes in, I only had the hole opened for a second while injecting spores then re-sealing. I will post more questions/comments later, as this is the first and probably hardest step in the process (imho).


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InvisibleHippie3
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Re: Honey jar help
    #85576 - 05/22/00 09:20 AM (16 years, 6 months ago)

where did you get your spores ?
in a post at drooldonkey,
workman mentioned that most vendors', other than pf, have yeasts in their syringes.

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