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 WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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 Re: Honey jar help  [Re: Hippie3]
#85577 - 05/22/00 11:43 AM (23 years, 4 months ago) |
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I got it from PF, which I believe I mentioned earlier in this thread.Still, no one has answered me, how can I not suck in that air when opening? Can I not tape the hole, put tin foil over the jar to keep at water, spray lysol everywhere, open the lid of the pan, inject fast, and then cover with tape? I am almost positive this is the crap from the air, I heard air rush in there when I punctured the hole, I am sure.
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,'~Þ
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 CLuB99
lost somewhere in time and space
 
Registered: 10/26/99
Posts: 1,316
Loc: my mind
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Re: Honey jar help [Re: Hippie3]
#85578 - 05/22/00 12:35 PM (23 years, 4 months ago) |
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WildLASER
Maybe this can help you....
You can drill 2 holes in the lid and use one to inject spores/suck liquid mycelium and the other one for gas exchange as shown in the the pic, also in this way when you suck or inject the difference of pressure will be neutralized.
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Look at http://novenove.homepage.com/Honeytek.html for the liquidmycelium honeytek
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CLuB99
lost somewhere in time and space
Registered: 10/26/99
Posts: 1,316
Loc: my mind
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Re: Honey jar help [Re: Hippie3]
#85579 - 05/22/00 12:37 PM (23 years, 4 months ago) |
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Damn pics.....let's try again...
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CLuB99
lost somewhere in time and space
Registered: 10/26/99
Posts: 1,316
Loc: my mind
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Re: Honey jar help [Re: Hippie3]
#85580 - 05/22/00 12:38 PM (23 years, 4 months ago) |
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WTF????????
ok...go to the link in my signature, better way 
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85581 - 05/22/00 04:01 PM (23 years, 4 months ago) |
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Yo, thanks, although still, I must be quick and not let much air get near the hole on top, I think I will make that hole with a filter, and then cover my other hole with tape, puncture it, inject real fast, (maybe while still in pan with lysol sprayed around it?) then cover up fast with tape again. Hopefully this will work...
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,'~Þ
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85582 - 05/23/00 03:06 PM (23 years, 4 months ago) |
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I am not sure if my filters are good enough? Are the ones from FP good enough, ChesterCopperpot sent them to me.
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Anonymous
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Re: Honey jar help [Re: Hippie3]
#85583 - 05/23/00 04:52 PM (23 years, 4 months ago) |
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whats the yeast for in the syringe
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85584 - 05/28/00 10:42 PM (23 years, 4 months ago) |
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I'd imagine the yeast got in there by mistake, or is used to preserve or something? Personally, I see no reason, wouldn't yeast just overrun the mycelium?
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,'~Þ
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CLuB99
lost somewhere in time and space
Registered: 10/26/99
Posts: 1,316
Loc: my mind
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Re: Honey jar help [Re: Hippie3]
#85585 - 05/29/00 11:17 AM (23 years, 4 months ago) |
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Hey WildLASER....
Put a drop of silicone on the other hole, instead of using tape.....
Try it!!! it works much better (- less contams)
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85586 - 05/30/00 07:06 PM (23 years, 4 months ago) |
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I just used one hole and didnt tape it, I covered with aluminum foil, but I think since it was sitting there for so long it might have messed up. I think this new jar is bad too, I just got a new syringe, looks much better (healthier) then the old one.
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85587 - 05/31/00 07:15 PM (23 years, 4 months ago) |
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I think I will just tape a filter over the hole, (tape the edges) then quickly insert the syringe and inject, and cover with the filter and tape over it. I am using the new syringe I have which should be VIABLE spores this time, as the syringe looks much healthier.
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,'~Þ
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85588 - 05/31/00 07:18 PM (23 years, 4 months ago) |
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[wrong thread Shroomery and IE messed up][This message has been edited by WildLASER (edited May 31, 2000).]
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85589 - 06/02/00 02:47 PM (23 years, 4 months ago) |
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Can anyone tell me a FOOLPROOF way, I have 3 jars failed so far, don't want to lose any more spore solution or time.
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Anonymous
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Re: Honey jar help [Re: Hippie3]
#85590 - 06/02/00 03:26 PM (23 years, 4 months ago) |
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Try inoculating using the oven tek. Unfortunately, this is not foolproof method. If you want to get closer to foolproof, just stick with inoculating your jars with the spore solution. The honey water or malt extract water should only need a few drops of spore solution, so the risk isn't that great. 
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"It never got weird enough for me."
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Hippie3
mycotopiate
Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
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Re: Honey jar help [Re: Hippie3]
#85591 - 06/02/00 04:11 PM (23 years, 4 months ago) |
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there is no 'fool-proof' way but
try working in a glovebox,
use filter disk/material to prevent air rushing in,
and incubate in a clean environment.
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Admin @ mycotopia.net
Mycotopia
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WildLASER
Infinity In AGrain Of Salt
Registered: 04/01/00
Posts: 870
Last seen: 3 years, 9 months
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Re: Honey jar help [Re: Hippie3]
#85592 - 06/02/00 06:51 PM (23 years, 4 months ago) |
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Thanks, I think I may construct a glove box, or use the oven, but I am definetly using a filter disc, I have some from FP that were sent to me by a generous person. I also have some fine porosity filters from the my chem class (was a bio room too).
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keyzar
journeyman
Registered: 04/03/00
Posts: 56
Last seen: 21 years, 6 months
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Re: Honey jar help [Re: Hippie3]
#85593 - 06/02/00 07:38 PM (23 years, 4 months ago) |
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i have had no luck with the cambodians and the honey tek i did 4 jars 1 cambodians 1 of the b+ 1 golden teachers 1 azure the cambodians never germinated in the honey water but they did fine on malt agar so im going to try them on malt water solution the golden teachers took off and the b+ took off both with great sucsess the azures didnt do so well so i tried them in a tea of sorts that i made out of ground up oak bark and a little honey boiled it and strained it in a coffee filter only a little sediment remains and interestingly enough the mycelium seems to be growing off the bark sediment but still no luck getting the cambodians to germinate in honeywater
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