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Zwieback0
Baby Bread
Registered: 08/23/02
Posts: 3,473
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Syringe Steriliity/Shake
#847377 - 08/27/02 04:09 PM (21 years, 7 months ago) |
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Ive read that to keep it safe you should sterilize your syringe, and it seems like using an alcohol flame is best. The thing is. After Sterilizing it do I wait for the tip to cool down then inoculate? And if you need to shake up the spores inside the syringe and there is no air in there, what would be the safest way to get air in without contaminating it?
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Mr_Puke
Suicidal Failure
Registered: 06/11/02
Posts: 673
Last seen: 20 years, 5 months
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
#847401 - 08/27/02 04:19 PM (21 years, 7 months ago) |
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Once you wipe the needle with the alcohol it will cool the needle. If not you can wait, only takes seconds to cool. And if you want air in your syringe, put a flame to the needle and pull the plugger back, thus giving you "sterile" air.
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Macey Howard
Formally MOE HOWARD
Registered: 07/02/99
Posts: 14,165
Loc: Georgia
Last seen: 7 years, 6 months
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Post deleted by Moe Howard [Re: Zwieback0]
#847450 - 08/27/02 04:42 PM (21 years, 7 months ago) |
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-------------------- Hugs and Kisses!
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Zwieback0
Baby Bread
Registered: 08/23/02
Posts: 3,473
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Re: Syringe Steriliity/Shake [Re: Mr_Puke]
#847452 - 08/27/02 04:43 PM (21 years, 7 months ago) |
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Wouldnt pulling the air while the needle is over the flame too hot and therefore kill the spores inside the syringe?
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Syringe Steriliity/Shake [Re: Macey Howard]
#847454 - 08/27/02 04:43 PM (21 years, 7 months ago) |
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lmfaoooooooo @ Moe
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
#847456 - 08/27/02 04:44 PM (21 years, 7 months ago) |
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no it won't.....you are just pulling in air.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
#847496 - 08/27/02 04:59 PM (21 years, 7 months ago) |
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What I do is wipe the needle with alcohol as everyone else suggested, then I squirt a little "spore juice" out of the needle. Cools everything quickly.
-------------------- "I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
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I_Stain_Blue
abominableshroomman
Registered: 08/19/02
Posts: 395
Loc: police state
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Re: Syringe Steriliity/Shake [Re: mickey_rourke]
#847926 - 08/27/02 07:40 PM (21 years, 7 months ago) |
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yea, and theres a cool burst of hot steam!
do you wipe with an alcohol soaked cloth? i dont have any alcohol pads
-------------------- Be Here Now
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Poopman
Man of Poop
Registered: 04/04/02
Posts: 643
Loc: Los Angeles
Last seen: 15 years, 2 months
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Re: Syringe Steriliity/Shake [Re: I_Stain_Blue]
#847936 - 08/27/02 07:45 PM (21 years, 7 months ago) |
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And the stench of little dying spore babies gettin incinerated.
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: Syringe Steriliity/Shake [Re: Poopman]
#847939 - 08/27/02 07:47 PM (21 years, 7 months ago) |
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When you have spores by the thousands, it doesn't really matter now does it?
-------------------- "I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
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canid
irregular meat sprocket
Registered: 02/26/02
Posts: 11,912
Loc: looking for zeebras, n. c...
Last seen: 2 months, 17 days
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
#847949 - 08/27/02 07:51 PM (21 years, 7 months ago) |
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what do you need air to shake for? albiet it helps but it's not nessecary.
-------------------- Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it. If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Syringe Steriliity/Shake [Re: canid]
#848045 - 08/27/02 08:26 PM (21 years, 7 months ago) |
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I never have to put air into my syringes to shake them.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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funwithgus
member
Registered: 07/17/02
Posts: 196
Loc: Philadelphia area
Last seen: 16 years, 10 months
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Re: Syringe Steriliity/Shake [Re: I_Stain_Blue]
#848087 - 08/27/02 08:53 PM (21 years, 7 months ago) |
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Buy alcohol at the drug store and use napkins or paper towels. They've worked so far for my cat.
-------------------- Say, can I have some of your purple berries? Yes, I've been eating them, for six or seven weeks now, haven't got sick once.
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Syringe Steriliity/Shake [Re: funwithgus]
#848090 - 08/27/02 08:56 PM (21 years, 7 months ago) |
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I like using 99% alcohol and cotton balls.
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Macey Howard
Formally MOE HOWARD
Registered: 07/02/99
Posts: 14,165
Loc: Georgia
Last seen: 7 years, 6 months
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Post deleted by Moe Howard [Re: Roadkill]
#848098 - 08/27/02 09:00 PM (21 years, 7 months ago) |
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-------------------- Hugs and Kisses!
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Roadkill
Retired Shroomery Mod
Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Syringe Steriliity/Shake [Re: Macey Howard]
#848101 - 08/27/02 09:02 PM (21 years, 7 months ago) |
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I have an alcohol lamp....I just like cotton balls better!
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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