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InvisibleZwieback0
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Registered: 08/23/02
Posts: 3,473
Syringe Steriliity/Shake
    #847377 - 08/27/02 06:09 PM (14 years, 3 months ago)

Ive read that to keep it safe you should sterilize your syringe, and it seems like using an alcohol flame is best. The thing is. After Sterilizing it do I wait for the tip to cool down then inoculate? And if you need to shake up the spores inside the syringe and there is no air in there, what would be the safest way to get air in without contaminating it?


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OfflineMr_Puke
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Registered: 06/11/02
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
    #847401 - 08/27/02 06:19 PM (14 years, 3 months ago)

Once you wipe the needle with the alcohol it will cool the needle. If not you can wait, only takes seconds to cool. And if you want air in your syringe, put a flame to the needle and pull the plugger back, thus giving you "sterile" air.


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OfflineMacey Howard
Formally MOE HOWARD
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Registered: 07/03/99
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Post deleted by Moe Howard [Re: Zwieback0]
    #847450 - 08/27/02 06:42 PM (14 years, 3 months ago)



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InvisibleZwieback0
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Registered: 08/23/02
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Re: Syringe Steriliity/Shake [Re: Mr_Puke]
    #847452 - 08/27/02 06:43 PM (14 years, 3 months ago)

Wouldnt pulling the air while the needle is over the flame too hot and therefore kill the spores inside the syringe?


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InvisibleRoadkill
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,598
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Re: Syringe Steriliity/Shake [Re: Macey Howard]
    #847454 - 08/27/02 06:43 PM (14 years, 3 months ago)

lmfaoooooooo @ Moe


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleRoadkill
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
    #847456 - 08/27/02 06:44 PM (14 years, 3 months ago)

no it won't.....you are just pulling in air.


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Invisiblemickey_rourke
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Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: Syringe Steriliity/Shake [Re: Zwieback0]
    #847496 - 08/27/02 06:59 PM (14 years, 3 months ago)

What I do is wipe the needle with alcohol as everyone else suggested, then I squirt a little "spore juice" out of the needle. Cools everything quickly.


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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InvisibleI_Stain_Blue
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Registered: 08/19/02
Posts: 395
Loc: police state
Re: Syringe Steriliity/Shake [Re: mickey_rourke]
    #847926 - 08/27/02 09:40 PM (14 years, 3 months ago)

yea, and theres a cool burst of hot steam!


do you wipe with an alcohol soaked cloth? i dont have any alcohol pads


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OfflinePoopman
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Registered: 04/04/02
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Re: Syringe Steriliity/Shake [Re: I_Stain_Blue]
    #847936 - 08/27/02 09:45 PM (14 years, 3 months ago)

And the stench of little dying spore babies gettin incinerated.


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Invisiblemickey_rourke
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Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
Re: Syringe Steriliity/Shake [Re: Poopman]
    #847939 - 08/27/02 09:47 PM (14 years, 3 months ago)

When you have spores by the thousands, it doesn't really matter now does it?


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"I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five


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Offlinecanid
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Re: Syringe Steriliity/Shake [Re: Zwieback0]
    #847949 - 08/27/02 09:51 PM (14 years, 3 months ago)

what do you need air to shake for? albiet it helps but it's not nessecary.


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Attn PWN hunters: If you should come across a bluing Psilocybe matching P. pellicolusa please smell it.
If you detect a scent reminiscent of Anethole (anise) please preserve a specimen or two for study and please PM me.


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InvisibleRoadkill
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Re: Syringe Steriliity/Shake [Re: canid]
    #848045 - 08/27/02 10:26 PM (14 years, 3 months ago)

I never have to put air into my syringes to shake them.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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Offlinefunwithgus
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Registered: 07/17/02
Posts: 196
Loc: Philadelphia area
Last seen: 9 years, 6 months
Re: Syringe Steriliity/Shake [Re: I_Stain_Blue]
    #848087 - 08/27/02 10:53 PM (14 years, 3 months ago)

Buy alcohol at the drug store and use napkins or paper towels. They've worked so far for my cat.


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Say, can I have some of your purple berries?
Yes, I've been eating them, for six or seven weeks now, haven't got sick once.


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InvisibleRoadkill
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Re: Syringe Steriliity/Shake [Re: funwithgus]
    #848090 - 08/27/02 10:56 PM (14 years, 3 months ago)

I like using 99% alcohol and cotton balls.


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMacey Howard
Formally MOE HOWARD
Female

Registered: 07/03/99
Posts: 14,165
Loc: Georgia
Last seen: 3 months, 6 days
Post deleted by Moe Howard [Re: Roadkill]
    #848098 - 08/27/02 11:00 PM (14 years, 3 months ago)



--------------------
Hugs and Kisses!


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InvisibleRoadkill
Retired Shroomery Mod
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Registered: 12/11/01
Posts: 22,598
Loc: Snoqualmie, Wa.
Trusted Cultivator
Re: Syringe Steriliity/Shake [Re: Macey Howard]
    #848101 - 08/27/02 11:02 PM (14 years, 3 months ago)

I have an alcohol lamp....I just like cotton balls better!


--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



Post Extras: Print Post  Remind Me! Notify Moderator
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