Spore Syringe Supplies:
(For Plastic Bag TEK) 1 PC 1 glove box 4 unopened sterile syringes – 18 gauge, 3ml 1 fresh spore print 3 heat resistant plastic zip lock bags (Smelly Proof brand) 1 quart jar 1 gallon of distilled water
(For Shot Glass TEK – as back-up) 1 shot glass 1 ½ pint jar foil
Sanitary supplies:
Plastic sheeting Oust and Lysol 10% bleach water / sprayer / towel 50% bleach water / sprayer 10 sealed alcohol wipes Denatured alcohol flame (may not use) Lighter (may replace alcohol fame, but also may not use)
First, the kitchen area is very small, but not totally sterile. I will start by a good kitchen cleaning and basic wipe down with 50/50 bleach water and Lysol. I will also be using the plastic sheeting to cover the window and cover the doorway to control air flow and cover a few cabinets.
Once everything is wiped down and surfaces are prepared, I’ll get everything ready for the Pressure Cooker.
To start, I will add take three plastic zip lock bags and fill each with 12ml of distilled water, seal 2 and leave one slightly open, and place both into a quart jar with lid and seal.
Incase there is a problem with the baggies; I will also prepare a shot glass, wrapped in foil, placed in a pint jar with lid and seal. I will also add 12ml to a ½ pint jar with lid and seal.
All this will go into the PC for 1 hour at 15psi.
While the PC is doing its thing, I will continue to decontaminate the room, wiping down surfaces with 50/50 bleach water and Lysol.
Also at this time I will completely sterilize my glove box with 50/50 bleach water. And add a wet 10% bleach water towel to the bottom, along with the syringes, spore print, alcohol wipes, flame/lighter, and 10% bleach water sprayer
Once the PC is done, I will follow the direction and prepare to open it ASAP. During the 10 minute wait after removing the PC bobble, I’ll spray down the air with Oust and get a good cloud / mist in the air right before opening. While everything in the PC is still hot and the oust mist is in the air, I’ll place everything in the glove box and seal it.
Once the glove box is sealed, I’ll use the 10% bleach water spray to spray down the inside one more time and let everything cool for at least 3 hours (I may add a cold pack in the glove box, sealed in a plastic bag and once the jars are cool to the touch, place them on top to make sure the water is well cooled.
Once everything is cooled, I’ll make the syringe.
I’ll open the jar with the plastic bags and check them to make sure they have not been corrupted by the PC in some way. Hopefully one of the sealed bags will remain sealed. I’ll use the best of the three.
I’ll carefully open part of the bag top, being careful keep the opening facing down; then take the spore print out of its sealed bag and quickly toss it into the baggy and reseal it.
Being careful (the prints are on foil) I will rub the spores off the foil and mix it around pretty good.
Once mixed, I will wipe down one corner of the bag with alcohol. Then quickly open the sterile syringe, take off the top, and insert it into the baggy, pull up 3 ml, pull out, wipe the needle with alcohol (I see no real need to flame using this TEK), replace the cap and tape it on.
Once one syringe is full, I will repeat with the remaining three needles, however, I will wipe down new spots on the baggy for each needle and be very careful of the various holes.
The baggy TEK seems easiest, but I’m a newb too.
If I default to the shot glass TEK,
I’ll open the jar with the shot glass, remove the foil. Then I will open the water jar and syringe and pull-up 3ml of water, wipe down the needle. Then open the spore print and using the needle, scrape off ¼ of the print into the shot glass. Then with the syringe, put half the water in, mix and pull back up – with a little more in and out to break the spores. Once full, wipe once again and replace the cap and tap it on.
Then repeat three more times. As a newb, the baggy TEK seem easiest, with only a few second of exposure compared to the shot glass TEK.
Input, Ideas, Feedback. Am I missing anything here?
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