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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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fearforce7
addict
Registered: 01/30/00
Posts: 642
Loc: USA
Last seen: 9 years, 23 days
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I am scared to do a Print/syring!
#829024 - 08/19/02 07:07 PM (22 years, 8 months ago) |
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I don't know why but i keep thinking i am gonna have trouble making a sterile print and syring. I have grown the mushrooms, they should be done by this friday, but i am worried that i will not be able to make a sterile print and syring. What is the best method to work for you guys, for printing and syringe making?
I have printed more the 10 teks on this but can't pick one.
So i need input.
thanks ff7
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twisted
member

Registered: 06/15/02
Posts: 177
Loc: croatia
Last seen: 13 years, 10 months
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Re: I am scared to do a Print/syring! [Re: fearforce7]
#829045 - 08/19/02 07:18 PM (22 years, 8 months ago) |
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why thats plain old silly you got nothing to worry about
-------------------- Jesus! Did I say that?Or just think it? Was i talking ? Did they hear me?
http://www.fsre.org/
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mickey_rourke
Illegal Smile
Registered: 07/18/02
Posts: 3,333
Loc: Playa del Carmen
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Re: I am scared to do a Print/syring! [Re: fearforce7] 1
#829048 - 08/19/02 07:19 PM (22 years, 8 months ago) |
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This is what I do. I harvest a mushroom, and remove the cap from the stem with a clean scalpel. I sterilize some foil and place it on a clean, hard surface that has been sprayed with Lysol. I then place the cap on the sterilized foil and cover it with a clean bowl that has been wiped with alcohol. Let the cap print overnight. The next morning, spray the area with lysol and remove the cap. Take the foil and quickly fold it up and over itself to seal. Voila! Clean print.
When I make a syringe, I fill up a 1/8 pint canning jar with the amount of water that I need. The lid of this jar has been modified with a hole in the center. I usually make this about 1/4" or so. Cover the hole with masking tape, and cover the entire top with foil. I then PC for 30 minutes and allow the jar to cool. When the jar is cool, I turn the oven on low and pull out the lowest tray to use as a work surface. Wipe the jar with alcohol and remove the foil from the jar. Open the lid and scrape the spores from the print into the jar -- for most prints, an area the size of a match head works well for each syringe. Be sure and do this as quickly as possible. Put the lid back on and remove the masking tape. Stick a syringe into the hole and fill it. Squirt the water back in as soon as it's filled and repeat a few times. This breaks up the spores and distributes it evenly into the water. Fill syringes and replace cover on the needle. Slap a piece of tape on it and record the strain and date.
Good luck! (This was my 666th post. )
-------------------- "I tried to put it all behind me, but my redneck past is nipping at my heels.." -- Ben Folds Five
Edited by mickey_rourke (08/19/02 07:25 PM)
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Dutchbrewed
Metaphysical



Registered: 06/29/09
Posts: 289
Last seen: 8 years, 5 months
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Re: I am scared to do a Print/syring! [Re: mickey_rourke]
#10668493 - 07/13/09 11:17 AM (15 years, 9 months ago) |
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I like what Mickey had to say.... mine's just slightly different, but it sounds like he found a good way to cut some lengthy corners in sterilization, so I say go for it. Cultivating mushrooms is really a learn as you go kind of thing anyway. If you fail, deduce the problem and attack it again. I have faith in you
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 3 months
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Re: I am scared to do a Print/syring! [Re: mickey_rourke]
#10668513 - 07/13/09 11:20 AM (15 years, 9 months ago) |
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Quote:
mickey_rourke said: This is what I do. I harvest a mushroom, and remove the cap from the stem with a clean scalpel. I sterilize some foil and place it on a clean, hard surface that has been sprayed with Lysol. I then place the cap on the sterilized foil and cover it with a clean bowl that has been wiped with alcohol. Let the cap print overnight. The next morning, spray the area with lysol and remove the cap. Take the foil and quickly fold it up and over itself to seal. Voila! Clean print.
When I make a syringe, I fill up a 1/8 pint canning jar with the amount of water that I need. The lid of this jar has been modified with a hole in the center. I usually make this about 1/4" or so. Cover the hole with masking tape, and cover the entire top with foil. I then PC for 30 minutes and allow the jar to cool. When the jar is cool, I turn the oven on low and pull out the lowest tray to use as a work surface. Wipe the jar with alcohol and remove the foil from the jar. Open the lid and scrape the spores from the print into the jar -- for most prints, an area the size of a match head works well for each syringe. Be sure and do this as quickly as possible. Put the lid back on and remove the masking tape. Stick a syringe into the hole and fill it. Squirt the water back in as soon as it's filled and repeat a few times. This breaks up the spores and distributes it evenly into the water. Fill syringes and replace cover on the needle. Slap a piece of tape on it and record the strain and date.
Good luck! (This was my 666th post. <img src="/forums/images/icons/smile.gif" alt="" />)
Let your print dry for 24 hours before you seal it up.
its very annoying recieving a print that the spores are stuck together in a sheet and from huge clumps in a syringe. plus nasty things can happen to a wet print.
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek
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phaseflux
Stranger than most


Registered: 07/16/08
Posts: 702
Last seen: 11 years, 6 months
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Re: I am scared to do a Print/syring! [Re: Dutchbrewed]
#10668518 - 07/13/09 11:21 AM (15 years, 9 months ago) |
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haha don't be scared. In the beginning I had to start off from a print and make my own spore syringe. Anyway, i was sure it was gonna be a failure, but everything worked out fine. . Want some advice? Use a glovebox/still air box.
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phaseflux
Stranger than most


Registered: 07/16/08
Posts: 702
Last seen: 11 years, 6 months
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Re: I am scared to do a Print/syring! [Re: veda_sticks]
#10668530 - 07/13/09 11:23 AM (15 years, 9 months ago) |
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Quote:
veda_sticks said:
Let your print dry for 24 hours before you seal it up.
its very annoying recieving a print that the spores are stuck together in a sheet and from huge clumps in a syringe. plus nasty things can happen to a wet print.
And do listen to this. I've ruined quite a few prints this way. Luckily I have a lot to work with still.
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bob psilocybe
Stranger
Registered: 06/06/12
Posts: 3
Last seen: 12 years, 6 months
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Re: I am scared to do a Print/syring! [Re: fearforce7] 1
#16342378 - 06/06/12 05:32 PM (12 years, 10 months ago) |
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Easiest way that worked for me is take some clean forceps and scissors, cut cap of mushroom place in small sterile jar (just bake empty jar 350 - 40 min or less) and allow it to make a spore print at the bottom (24 hrs later) take the cap out and add filtered water carefully then close and shake and you have your own spore water - this tech saved me hundreds of bucks
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Mugnath



Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 8 months, 1 day
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Re: I am scared to do a Print/syring! [Re: fearforce7]
#16342477 - 06/06/12 05:55 PM (12 years, 10 months ago) |
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I use Multisync's tek personally, I find it to be pretty simple, and I have not had any contam errors (except one with cobweb due to improper inoculation. Just a bag and some scissors is all that's required.
http://www.shroomery.org/9210/MultiSyncs-lazy-bastard-print-syringe-guide
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: I am scared to do a Print/syring! [Re: Mugnath]
#16342501 - 06/06/12 05:59 PM (12 years, 10 months ago) |
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lol! u realize this thread is nearly 3 years old, of course. i'm guessing the op figured it out or quit. u could ask them, but they haven't been seen 'round these parts for 7 months.
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Mugnath



Registered: 05/24/12
Posts: 733
Loc: Oregon
Last seen: 8 months, 1 day
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Re: I am scared to do a Print/syring! [Re: Fungal growth]
#16342507 - 06/06/12 06:00 PM (12 years, 10 months ago) |
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Quote:
Fungal growth said: lol! u realize this thread is nearly 3 years old, of course. i'm guessing the op figured it out or quit. u could ask them, but they haven't been seen 'round these parts for 7 months.
Lol woops, didn't notice.
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McD
Boss

Registered: 07/02/12
Posts: 1
Last seen: 3 years, 8 months
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Re: I am scared to do a Print/syring! [Re: Mugnath]
#16567304 - 07/22/12 09:44 AM (12 years, 8 months ago) |
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I've spent the past few days looking at various syringe teks for my first attempt, which I'm gradually building up the confidence for. (Shouldn't be much longer now!) I found it very useful. It's going into a pot in my head with a few other selected teks where it will be mixed, digested and hopefully come out as my own successful new tek.
So... Thanks again!
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Revolutiontcm
dniM-retsaM



Registered: 04/05/12
Posts: 949
Loc: MD
Last seen: 7 months, 13 days
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Re: I am scared to do a Print/syring! [Re: McD]
#16567815 - 07/22/12 11:55 AM (12 years, 8 months ago) |
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#829024 - 08/19/02 09:07 PM (9 years, 10 months ago)
How do you guys come across these old threads?
--------------------

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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: I am scared to do a Print/syring! [Re: McD]
#16567894 - 07/22/12 12:14 PM (12 years, 8 months ago) |
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Quote:
McD said: I've spent the past few days looking at various syringe teks for my first attempt, which I'm gradually building up the confidence for. (Shouldn't be much longer now!) I found it very useful. It's going into a pot in my head with a few other selected teks where it will be mixed, digested and hopefully come out as my own successful new tek.
So... Thanks again!
lol! day of the dead. so, you've never gown before, but your gonna look over some grows then not follow any, instead you'll mix em up and come up with a new way of doing things? pretty bold for your first grow. if past noobish failures are any indication, you'll be sailing away.
 toottoot!
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Synapse Trap
Eating God's Holy Cannabis Plant


Registered: 02/19/12
Posts: 1,698
Last seen: 9 years, 9 months
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Re: I am scared to do a Print/syring! [Re: Fungal growth]
#16567977 - 07/22/12 12:35 PM (12 years, 8 months ago) |
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He didn't say he's never grown before.. I took it to mean his first attempt at making a syringe. I give him a D+ for communication, but I think he's saying he's going to take the best of various teks and find a method that works best for him.
-------------------- Synapse Trap
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Fungal growth
Lootinint



Registered: 03/21/10
Posts: 3,641
Loc: under a rock in your yard
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Re: I am scared to do a Print/syring! [Re: Synapse Trap]
#16568062 - 07/22/12 12:52 PM (12 years, 8 months ago) |
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o. my bad. i didn't realize there was more than 1 way to make a syringe properly. boil H2O for 10 minutes, suck up H2O into syringe and purge 3-5 or so times, fill with boiling H2O and let sit for 10 minutes. purge. i fill and purge a few more times, then fill one final time, then let it cool. i could do it in my sleep. works every time.
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God Stamet
wannabe Stamets


Registered: 07/01/12
Posts: 774
Last seen: 10 years, 5 months
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Re: I am scared to do a Print/syring! [Re: Fungal growth]
#16568088 - 07/22/12 12:56 PM (12 years, 8 months ago) |
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-------------------- I have caught the Mycology bug! Not just here for the ride, i'm here for life.
How I do my bulk substrate
just another mushroom capsule TEK
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zenarrow
Stranger


Registered: 02/09/17
Posts: 292
Last seen: 8 months, 20 days
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Re: I am scared to do a Print/syring! [Re: fearforce7]
#24117024 - 02/24/17 07:04 PM (8 years, 1 month ago) |
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Gonna save this page for future reference
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 1 month, 23 days
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Re: I am scared to do a Print/syring! [Re: zenarrow]
#24117054 - 02/24/17 07:11 PM (8 years, 1 month ago) |
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Learn to build and utilize a still air box first !
--------------------

Amateur Mycologists United
AMU Q&A
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Wispy Wizard
Wilderness Junkie

Registered: 12/12/18
Posts: 12
Last seen: 6 years, 3 months
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Re: I am scared to do a Print/syring! [Re: zenarrow]
#25733976 - 01/09/19 01:37 PM (6 years, 3 months ago) |
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Agreed. Following.
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axxron11
Stranger


Registered: 10/23/19
Posts: 42
Loc: FL
Last seen: 4 years, 9 months
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Re: I am scared to do a Print/syring! [Re: bob psilocybe]
#26309996 - 11/09/19 06:33 PM (5 years, 5 months ago) |
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Baking the cut shroom peice doesn't hurt the spores?
-------------------- Just a Hippie lovin life and broadening my insight.
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FungiMaster
Entrepreneur



Registered: 10/31/19
Posts: 998
Loc: Bay Area, CA, USA
Last seen: 5 years, 4 months
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Re: I am scared to do a Print/syring! [Re: axxron11]
#26310253 - 11/09/19 08:10 PM (5 years, 5 months ago) |
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um u ain't supposed to bake the shroom. Just cut off the head and put it on foil, and wait a day or two.
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tfortorment
Stranger
Registered: 04/19/20
Posts: 1
Last seen: 4 years, 10 months
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Re: I am scared to do a Print/syring! [Re: mickey_rourke]
#26622450 - 04/23/20 02:00 PM (4 years, 11 months ago) |
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Quote:
mickey_rourke said: This is what I do. I harvest a mushroom, and remove the cap from the stem with a clean scalpel. I sterilize some foil and place it on a clean, hard surface that has been sprayed with Lysol. I then place the cap on the sterilized foil and cover it with a clean bowl that has been wiped with alcohol. Let the cap print overnight. The next morning, spray the area with lysol and remove the cap. Take the foil and quickly fold it up and over itself to seal. Voila! Clean print.
When I make a syringe, I fill up a 1/8 pint canning jar with the amount of water that I need. The lid of this jar has been modified with a hole in the center. I usually make this about 1/4" or so. Cover the hole with masking tape, and cover the entire top with foil. I then PC for 30 minutes and allow the jar to cool. When the jar is cool, I turn the oven on low and pull out the lowest tray to use as a work surface. Wipe the jar with alcohol and remove the foil from the jar. Open the lid and scrape the spores from the print into the jar -- for most prints, an area the size of a match head works well for each syringe. Be sure and do this as quickly as possible. Put the lid back on and remove the masking tape. Stick a syringe into the hole and fill it. Squirt the water back in as soon as it's filled and repeat a few times. This breaks up the spores and distributes it evenly into the water. Fill syringes and replace cover on the needle. Slap a piece of tape on it and record the strain and date.
Good luck! (This was my 666th post. <img src="/forums/images/icons/smile.gif" alt="" />)
wait, why do this in an oven? seems dangerous breathing all that gas in...
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LikeMyc

Registered: 12/06/19
Posts: 2,783
Last seen: 4 months, 25 days
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Re: I am scared to do a Print/syring! [Re: tfortorment]
#26622474 - 04/23/20 02:11 PM (4 years, 11 months ago) |
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This thread is 17 years old!
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poisoned
untitled



Registered: 04/17/13
Posts: 1,738
Loc: Yurop
Last seen: 2 years, 5 months
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Re: I am scared to do a Print/syring! [Re: LikeMyc]
#26622781 - 04/23/20 04:13 PM (4 years, 11 months ago) |
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I have never seen a thread resurrected so many times.
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aroundTheCorner
Some dude from around the corner



Registered: 07/25/09
Posts: 18
Last seen: 2 years, 8 months
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Re: I am scared to do a Print/syring! [Re: poisoned]
#27599531 - 12/30/21 07:43 AM (3 years, 3 months ago) |
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Me neither.
-------------------- -----------------------------------------------------------
For this is all a dream we dreamed one afternoon, long ago.
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LotKid
Never.Trust.A.Prankster



Registered: 01/07/17
Posts: 8,169
Loc: Shakedown St.
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You just had to do it... didnt you?
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Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,352
Loc: Northeast USA
Last seen: 22 days, 15 hours
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Re: I am scared to do a Print/syring! [Re: LotKid]
#27599598 - 12/30/21 08:54 AM (3 years, 3 months ago) |
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That was good.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------
Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
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