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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Easiest cultivation method ever!
    #7789758 -

OK I know there are numerous claims for methods which promise a super easy method to produce a psychedelic dose. But this is even more simple (and faster) than growing sclerotia on grass seed.

I have eaten two blueing mycelial mats of Ps. azurescens, each grown in a 250ml jar containing 100ml of malt agar (60grams of malt sugar, 2 grams of marmite, 2 grams of agar in one liter of boiling water, divided over ten 250mljars, each containing 100ml). Three weeks after inoculating the surface of the sterilized media, each inoculated with a colonized woodchip of azure mycelium and incubated at roomtemperature, all agars were completely covered with a thick mat of mycelium. The mycelium mat of two jars was about 40grams of wet mycelial mat in total and plenty for a very noticable effect to say the least.

With cubes I have never had that, and I have also never had that when I sampled some grain spawn. The trick to get thick mycelial mats is to use 2 grams of agar per liter insted of 20, and the method to get blueing mycelium of azurescens seems to be in the marmite.

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7789787 -

How was it compared to the same in wet cube mushies.

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Re: Easiest cultivation method ever! [Re: eman113]
    #7789872 -

"How was it compared to the same in wet cube mushies"

Definately more powerful than 1 gram dried of cubes, which is my treshold to see the paintings on the wall move.

I am baffled. Is this a phreak effect or is this real? IF this works the next time I am going for a PanCyan mycelial mat on 'soft' agar (PanCyan grows even faster than azure but is more blueing).

I really do hope that more people here want to try this out and post their experiences with soft agars (2g/l)

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7790000 -

^ try using a few gallons instead of litres. Would that work?


--------------------
"Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
            -wumen

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Re: Easiest cultivation method ever! [Re: navyseals101]
    #7790034 -

I just really can't understand your post. You are eating woodchips?

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Re: Easiest cultivation method ever! [Re: navyseals101]
    #7790050 -

"try using a few gallons instead of litres. Would that work?"

search for my other posts for the answer... In theory yes but in practice you get a bigger contam risk if you increase the surface of the medium. So single jars, each containing 100ml and requring one inoculation, look perfect to me. Every liter of soft agar medium will probably enough for some 200 grams of wet mycelium mat!

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7790058 -

No I am not eating woodchips. I used a single woodchip to inoculate each agar culture. And after harvesting the mats I didn't eat the inoculum but only the mats.

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7790088 -

Im very curious as to what you are talking about. Maybe, if your not busy, you can explain it as if you were explaining it to a very slow person. lol :smile:

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Re: Easiest cultivation method ever! [Re: Draigan]
    #7790137 -

"Im very curious as to what you are talking about. Maybe, if your not busy, you can explain it as if you were explaining it to a very slow person"

OK. I put 2 grams of agar, 60 grams of malt sugar (glucose/dextrose would probably also work) and 2 grams of marmite in a pan, add 1 liter of cold water, bring it to a boil and stir/dissove everything. Then I add ten ml of 3 percent hydrogen peroxide, and divide the contents over ten 250ml jars (100ml each). No glove box needed! Allow to cool down everything until the contents are cool to the touch and soldified into a kind of pudding. Add a single azurescens colonized woodchip to the agar surface in each jar by (using a flame-sterilized pair of tweezers for each chip). Close the lid on each jar loosely and allow them to stand for 3-4 weeks at roomtemperature. All jars now have a thick, blueing mycelial mat of 20 grams each. Eat two mats minus the woodchip which is used for inoculation. That is all.

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7790299 -

Cool idea but its kinda gross to eat a mat. Why not throw it in some 95% heat a little, let it sit and then drain it off threw a shirt or sock . Take whats there and let it evaporate to a drinkable amount. Its cool idea just need to make it more palatable even a tea wouldn't sound so nasty.Could take and squeeze the juice out and make kool-aid or something too and from the sounds of the color of the mat you could get some good blue juice out of those.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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Re: Easiest cultivation method ever! [Re: MYSTIQUE]
    #7790312 -

got any pic of you idea/ finish product


--------------------
NEVER DO THIS!!!

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! NO MORE BIG GOVERNMENT !

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Re: Easiest cultivation method ever! [Re: tonyshroom]
    #7790373 -

Yea man. This is a completely new tek. You should back it up with a more thought out explanation and some pictures. I am very interested.

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Re: Easiest cultivation method ever! [Re: DebuteMachine]
    #7790395 -

A new Tek? Not really Its a good idea but if you look into this you will see its already been done just to a totally finished product in the main cultivation info. The new mix sounds good for the agar but its not a totally new idea. No disrespect Ranonar.


--------------------
Dont know what the fuck I just said? READ THIS
http://www.shroomery.org/5122/The-Shroomery-Mushroom-Glossary
I ain't a hippy but I'm covered in dirt                         
Sippin lots of mushroom tea in a tye-dye shirt
Chasin' the Grateful Dead, no shoes on my feet
Beggin' in the parking lot for something to eat,

:onfire:DO NOT USE FIRE IN YOUR GLOVE BOX!!!!!!!:onfire:

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Re: Easiest cultivation method ever! [Re: MYSTIQUE]
    #7790568 -

I wouldn't eat a soggy mycelial mat either.  :scat:

But even so, nice system.


--------------------
Newcomers guide-----> For all things shroomy

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Re: Easiest cultivation method ever! [Re: Nibin]
    #7791466 -

"Yea man. This is a completely new tek."

Not new but near completely overlooked. Many people have tried eating their colonized agar media but this has no psychedelic effect because the mycelium only grows a very thin layer on media which contain 15-20 g/l of agar. Drinking liquid cultures has a similar drawback (not enough biomass).

But nice thick mats do form on soft agar (2g/l). And if you do not like to eat the mat you can easily pluck it from its medium, wash the water off, cut it in pieces, put it in a teabag and make tea (or bacardi blue rum).

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Re: Easiest cultivation method ever! [Re: ranonar]
    #7791587 -

Oh hell, I'm happy with my Tea.  That seems to fall in the same catagory as those that go through the trouble of making a mycelium shake.  GROSS!
That's just me:rolleyes:


--------------------


AMU Q&A thread.

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Re: Easiest cultivation method ever! [Re: Nibin] * 1
    #15425608 -

Auto-Summary
A post discussing mushroom cultivation and extraction yields was found, with a detailed yield of 452 grams of dried fungal material from 20 liters of gel, equivalent to 1083 milligrams of pure crystalline psilocybin and 45 milligrams of psilocin. The post also mentions alternative methods for growing and extracting psilocybin, including using H2O2 and submerged cultures, with potential for improved yields and reduced space requirements. The post concludes with a discussion on the potential for higher yields and stronger psilocybin content in mycelial tissue compared to mature mushrooms.

Just found this old thread, surprised it got so little attention.
Nibin said:
I wouldn't eat a soggy mycelial mat either.

The mats could be dried out.

There was a more detailed thread here
http://www.shroomery.org/forums/showflat.php/Number/7680813#7680813

cubensis works fine too, this was the yeild
Quote:
After incubation for 52 days at 24 centigrade in the dark, there are obtained 452 grams of dried fungal material, i.e. 22.6 grams per litre from a batch of 20 litres. The active substance is obtained from the fungal material in the manner described in Example 3. Yield: 1083 milligrams of pure crystalline psilocybin and 45 milligrams of psilocin.


This was from a 6% malt extract gel (with other additives). So just over 1gram of actives were produced, if your typical cube is 1% actives this is equivalent to 100g of dry cubes from 20L of gel. So this is like 5g of dry cubes per litre of gel. If you are putting 100ml in a half pint jar you could be getting the equivalent of 0.5g dry cubes in each.

This 0.5g per jar might not sound a lot but remember the jars can be totally stacked up, so less room needed, and of course less maintenance and additional equipment is needed etc.

I also wonder if many actives were lost during extraction. The yeild was 450g dry material which I equated to 100g dry cubes, so you could say the myc is only 1/4-1/5th as strong as the fruits. But if extraction was not efficient more would have been got (you could be eating the dry myc). Maybe there are other studies using the same extraction from fruits so you could get a better idea. e.g. if they got 450g dry cube fruits and got 2g actives then we could estimate the myc as, being half as strong as the fruits.

The studies talk of up to 10% sugar content so we might get more per litre. Also you could fit 200ml in a half pint jar so would this double the yeild?

I can see no real info about the ratio of surface area to volume. I would guess the more area the better.

If the H2O2 method really does work (I would have guessed it is too risky for contams) then there are loads of options due to no heating being needed. You could fill 2L PET soda bottles, fill them with about 1L and put them on their side to maximise exposed surface area, plug up the pouring hole with filter material. You could get 5L water containers and dump half the water out and pour in a concentrated malt mix with H2O2, this would minimise exposure to air. Some people have reported bottled water as being pretty clean to begin with. The concentrated sugar syrup could be boiled up really well, with no need to heat the final container (PET cannot withstand the heat).

If surface area ratio to volume is not so important we can grow upright. As the gel is easy and cheap to make you might get a better yeild from the same container. e.g. the study got 22.6g per litre of dry myc, I would guess they used a large surface area so you could expect 22.6g from your 2L bottle rested on its side with 1L. But if you could get say 30g from the same 2L bottle with 2L of gel in it, standing up with a smaller surface area, then it might be better. You have dropped your biological efficiency, but you get more yeild in the same space, and the upright bottles would be easier to store. The extra sugar cost is minimal.

Some sort of stackable trays could also be used, or 25L beer fermentation buckets. If contams were an issue even with H2O2 then the container could be the sterilization chamber as there is no need to PC. I suggested this before as ways of doing bulk LC or to fractionally sterilze non-exploding grains like RGS.

You would have a submersible stainless steel element in a fermentation bucket, put the ingredients in and boil it directly in the bucket with the lid on, and a filter. You have an injection port it is one giant 25L agar plate. There are many ways to do this, some home brewers can call these "keggles", where they convert beer kegs to kettles. YOu can buy ready made ones http://www.vinotheque.ca/english/equipment/equipment.cfm?equipment=294&category=Beer%20brewing%20equipment . You could make a clear window to keep an eye on it while growing. Many fermentation containers are translucent. This would be discrete enough too as you could tell people it is just beer you are brewing, you could brew beer alongside it to keep up appearances if needed. You can get brew belts and brew mats for homebrew which heat to cubensis incubation temps (if you need heating at all).


Here is another thread though
http://www.shroomery.org/forums/showflat.php/Number/4085512
Quote:
The mycelial tissue was found to produce psilocybin without having to go through the fruiting process... Even through the mycelial tissue grown from the Mexican species contained only half as much psilocybin (0.1-0.2% of dry weight) as the mushrooms did, high yields (20g/liter) along with the easy and rapid cultivation of mycelial tissue more than compensated for the lower psylocybin content

half as much would be fine by me.

BUT
Quote:
The submerged culture produced the highest amounts of biomass, followed by the surface cultures on soft agar (0.2%). But under both conditions the mycelia did not produce measurable amounts of alkaloids.

but they harvested after only 14 days, it may take longer for the actives to develop, many report stronger fruits from longer colonisation times. Tamp did grow stones and was active. It would be very easy to harvest sclerotia too.

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Re: Easiest cultivation method ever! [Re: blackout]
    #15440985 -

I was thinking you could probably make a semi solid solution of BRF with water to grow the myc on. It could have sugars added, they mention up to 10% malt extract, I imagine this could be added to the BRF too so it would be loaded with nutrient.

This would make harvesting sclerotia far easier. Which jogged my memory about sporeworks old "Philospher's Bags"

Quote:
Uninoculated Philospher's Bag - Consistancy is like modeling clay and appears to consist mostly of rice. Syringe passed through the injection port and easily into the substrate. When the inoculate was added it flowed out of the substrate and into the space between the substrate block and the bag. I used a full 10 cc syringe since I had it available and I wanted to speed things up. A few spots of growth on the surface of the substrate were visible in 4 days at 85F and quickly spread. It reminds me of mycelium growing on the surface of an agar plate.


Sounds like it had a very high moisture content, and the comment about the agar plate sounds like the mycelium mats discussed here.

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Re: Easiest cultivation method ever! [Re: blackout]
    #15440988 -

I was thinking you could probably make a semi solid solution of BRF with water to grow the myc on, to a similar consistency as the soft agar. It could have sugars added, they mention up to 10% malt extract, I imagine this could be added to the BRF too so it would be loaded with nutrient. And no need for agar, which some cannot get easily.

This would make harvesting sclerotia far easier. Which jogged my memory about sporeworks old "Philospher's Bags", specifically for sclerotia.

Quote:
Workman said:Uninoculated Philospher's Bag - Consistancy is like modeling clay and appears to consist mostly of rice. Syringe passed through the injection port and easily into the substrate. When the inoculate was added it flowed out of the substrate and into the space between the substrate block and the bag. I used a full 10 cc syringe since I had it available and I wanted to speed things up. A few spots of growth on the surface of the substrate were visible in 4 days at 85F and quickly spread. It reminds me of mycelium growing on the surface of an agar plate.


Sounds like it had a high moisture content, and the comment about the agar plate sounds like the mycelium mats discussed here.

Perhaps sporeworks went easy on the liquid so it would transport better in the post.





Edited by blackout (11/29/11 07:08 PM)

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Re: Easiest cultivation method ever! [Re: blackout]
    #15441672 -

I remember reading about this active mycelial mat method in the Anarchist Cookbook.
It said to dry out the mat, powder and put in gelcaps.


--------------------
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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