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OfflineSKrink
KING MOB
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Registered: 01/29/11
Posts: 1,042
Last seen: 10 years, 11 months
How To Use a Penis Envy Spore Swab * 10
    #15617015 - 01/04/12 07:36 PM (12 years, 26 days ago)

Having traded many Penis Envy spore swabs,
and being constantly asked "How Do I Use A Swab?"
I decided to make this guide, so that people who receive swabs from me or whoever,
have a better idea of what to do.

No, you do not just drop the swab into a grain jar.
ALWAYS START WITH AGAR!!
Because gill tissue (where the spores were swabbed) are usually prime carriers of bacteria and other contaminants.

If you received a Penis Envy spore swab from me, it probably looks something like this:


For this tutorial, I grabbed the oldest swab I have (4 months old) to show that viability is definitely not an issue.

So, let's get started.

1) PREPARE YOUR WORKSPACE

- Still Air Glove Box (Minimum requirement! Flowhood is best.)
- Agar petri dishes (I use 2% MEA)
- Penis Envy Spore Swab

You will have at least basic knowledge of working with agar and sterile technique,
so I don't need to go into every little detail of the preparation.
In any case, the info for agar basics is easily found on this site.


2) INOCULATE AGAR PLATE

- With one gloved, sanitized hand, take hold of the swab.
- With the other gloved, sanitized hand, lift up the agar plate cover.
- QUICKLY swipe/smear/rub the tip of the swab on your agar,
  confining your SWIFT movements to the middle of the plate.
- Replace agar plate cover
- Wrap the petri dish with parafilm (I use Saran wrap)
- Label clearly.


3) WAITING
Day 0

Some people say it's enough to swipe the swab across the agar once,
some people say brush, rub, or smear---
I like to give it a quick rub, kind of like I'm trying to remove a stain.
Anyway, I used the same swab to inoculate both plates, and labelled the plates "PEswab1" and PEswab2"

Day 7

PEswab1: Spores germinated, mycelium visible.
PEswab2: Nothing yet.

Day 9
 
PEswab1: Mycelial growth, contamination present (dark spot beside white mycelium)
PEswab2: Spores germinated, mycelium visible.

This contamination could have come from the swab itself, or more likely was picked up from the mushroom gills while swabbing.
Either way, this is why agar is essential when starting from spore swabs.

Day 13


Day 18
   
PEswab1: Mycelium has grown over the contaminant, appears to be otherwise healthy.
PEswab2: Completely clean, free of contaminants.


4) TRANSFER

I highly recommend doing at least one transfer, even if your culture appears to be clean.

In this case, although PEswab2 is squeaky clean, I prefer the way PEswab1's mycelium looks.

So I will do a transfer of the leading edge of mycelium in PEswab1, taking care to use areas far away from the site of initial contamination.
(Although the mycelium grew over the contam, it could still hitch a ride if the transfer site is too close)



Updates to come.


--------------------
:crazy2: SWEET POTATO HOME FRIES :crazy2:
:mushroom2:HOW TO USE A PENIS ENVY SPORE SWAB:mushroom2:

   

... Oh mighty masticator, salivator, vocalizer, swallower, licker biter sucker brow-knitter looker blinker rubbernecker thumber prodder up-yours fingerer ringwearer nosepicker waver drinker armlifter bodybender hipswiveler kneer springer runner ZERO::::::::OOOOOOOOO:::::::: RUN!!!


Edited by SKrink (01/04/12 07:38 PM)


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InvisibleBeefy1
GONE


Registered: 05/01/09
Posts: 3,573
Loc: around
Re: How To Use a Penis Envy Spore Swab [Re: SKrink]
    #15617068 - 01/04/12 07:45 PM (12 years, 26 days ago)

Very good tek.

this should find it's way into the archive


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Offlineboommer
Deforestation Operation


Registered: 12/30/11
Posts: 506
Loc: disney world
Last seen: 10 years, 3 months
Re: How To Use a Penis Envy Spore Swab [Re: Beefy1]
    #15617306 - 01/04/12 08:34 PM (12 years, 26 days ago)

nice tek man... good job


--------------------
"Great minds have purposes, little minds have wishes"
Dont have patience? BUILD A TIME MACHINE...
:pinkshroom: :supershroom: :regularshroom: :mushroomtwirl: :shroomeryhead: :scaryshroom:


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Invisibledtowntoker
gimme a spliff
Registered: 08/06/11
Posts: 2,368
Re: How To Use a Penis Envy Spore Swab [Re: boommer]
    #15617337 - 01/04/12 08:43 PM (12 years, 26 days ago)

Sweet, you should just continue this with a grow log.  I'd love to see some not fully isolated genetics and how the fruits look.  Lots of mutants maybe?


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OfflineDopsith
Male

Registered: 08/19/11
Posts: 102
Last seen: 2 years, 11 months
Re: How To Use a Penis Envy Spore Swab *DELETED* [Re: SKrink]
    #15617369 - 01/04/12 08:50 PM (12 years, 26 days ago)

Post deleted by Dopsith

Reason for deletion: Closed Account


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Offlinebrighteyedkitty
Hazee
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Registered: 07/12/10
Posts: 98
Last seen: 10 years, 7 months
Re: How To Use a Penis Envy Spore Swab [Re: Dopsith]
    #15617608 - 01/04/12 09:40 PM (12 years, 26 days ago)

good thread!


--------------------
My kitty Ludwig Haze and duck Bailey


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: How To Use a Penis Envy Spore Swab [Re: brighteyedkitty]
    #17463887 - 12/28/12 10:30 PM (11 years, 1 month ago)

Didn't wanna start a new thread, and this is a good one.

I just used a swab for the first time. I didn't read this guide, but did pretty much exactly the same thing. But it occured to me while using it (I did 3 plates) that you can't exactly flame sterilize your swab between plates. This got me to wondering - how many plates can you do with one swab? And is there any special technique to minimize contams while maximizing the number of plates you can do? Maybe just use different parts of the swab on each plate or something?


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 3 hours
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Re: How To Use a Penis Envy Spore Swab [Re: 36fuckin5] * 1
    #17464106 - 12/28/12 11:29 PM (11 years, 1 month ago)

I would rather cut off a small peice of the swab and smear with that, so the remaining spores on the swab can be stored for later use.

The way you are doing it now you would have to throw each swab away afterwards because the agar could make them germinate on the swab.

That's a lot of spores to be wasting. Especially if you only have 2 swabs.


Edited by PussyFart (12/28/12 11:30 PM)


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: How To Use a Penis Envy Spore Swab [Re: PussyFart]
    #17464222 - 12/28/12 11:59 PM (11 years, 1 month ago)

Quote:

Notahacker420 said:
I would rather cut off a small peice of the swab and smear with that, so the remaining spores on the swab can be stored for later use.

The way you are doing it now you would have to throw each swab away afterwards because the agar could make them germinate on the swab.

That's a lot of spores to be wasting. Especially if you only have 2 swabs.




That's a decent idea. Do you just use sterlized tweezers or something? And do you leave the piece of cotton on the plate, or just smear it and pick it back up?

I agree, it is a hell of a lot of spores to waste.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 3 hours
Trusted Cultivator
Re: How To Use a Penis Envy Spore Swab [Re: 36fuckin5] * 1
    #17464276 - 12/29/12 12:14 AM (11 years, 1 month ago)

Quote:

36fuckin5 said:
That's a decent idea. Do you just use sterlized tweezers or something? And do you leave the piece of cotton on the plate, or just smear it and pick it back up?

I agree, it is a hell of a lot of spores to waste.



Yes, just use some sterile tweezers, or scissors or something, and then smear and pick back up...i would do a few plates with each piece...but that's just me.


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Offlinedmonkey1
Monkey Say...
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Re: How To Use a Penis Envy Spore Swab [Re: dtowntoker]
    #17464302 - 12/29/12 12:23 AM (11 years, 1 month ago)

Quote:

dtowntoker said:
Sweet, you should just continue this with a grow log.  I'd love to see some not fully isolated genetics and how the fruits look.  Lots of mutants maybe?




this.  My mycelial judgement is just about the same as the OP.. prefer over looks.

What looks does PE mycelium have that represent good characteristics?
I have seen many non-fruiting PE strains come from MS agar work


--------------------
Check out my Trade List!!


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InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: How To Use a Penis Envy Spore Swab [Re: PussyFart] * 1
    #17464390 - 12/29/12 12:49 AM (11 years, 1 month ago)

Quote:

Notahacker420 said:
The way you are doing it now you would have to throw each swab away afterwards because the agar could make them germinate on the swab.




I've played with quite a few swabs in my time and I've never, ever thrown them out after one swipe.
And I've never heard of them germinating on the swab :shrug:

As long as you're working in front of a hood or in a SAB, you can easily repackage them in foil and use them another time.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: How To Use a Penis Envy Spore Swab [Re: dmonkey1]
    #17464393 - 12/29/12 12:49 AM (11 years, 1 month ago)

Quote:

dmonkey1 said:
Quote:

dtowntoker said:
Sweet, you should just continue this with a grow log.  I'd love to see some not fully isolated genetics and how the fruits look.  Lots of mutants maybe?




this.  My mycelial judgement is just about the same as the OP.. prefer over looks.

What looks does PE mycelium have that represent good characteristics?
I have seen many non-fruiting PE strains come from MS agar work




This thread is a year old.

Cloning will give more guarantees of a fruiting strain, but not the variety of overall strain choices that MS isolation will.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 3 hours
Trusted Cultivator
Re: How To Use a Penis Envy Spore Swab [Re: FrankHorrigan]
    #17464406 - 12/29/12 12:53 AM (11 years, 1 month ago)

Quote:

FrankHorrigan said:
Quote:

Notahacker420 said:
The way you are doing it now you would have to throw each swab away afterwards because the agar could make them germinate on the swab.




I've played with quite a few swabs in my time and I've never, ever thrown them out after one swipe.
And I've never heard of them germinating on the swab :shrug:



Hmmmm.... very interesting...  :philososloth:


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Offlinepsychodelicious
Turtle Man
Male


Registered: 12/30/15
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Loc: Earth C-137
Last seen: 3 days, 1 hour
Re: How To Use a Penis Envy Spore Swab [Re: SKrink]
    #27756522 - 04/29/22 12:35 PM (1 year, 8 months ago)

Commenting to save for later reference


--------------------


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OfflineMushieMafia
The Mushroom Don
I'm a teapot User Gallery


Registered: 02/15/22
Posts: 1,303
Loc: Down Under Mate
Last seen: 9 days, 9 hours
Re: How To Use a Penis Envy Spore Swab [Re: psychodelicious]
    #27915759 - 08/23/22 09:51 PM (1 year, 5 months ago)

what he said ^


--------------------
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