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Morel liquid culture
    #6766305 -

I cloned and started liquid cultures (with spores) of the morels from this thread: http://www.shroomery.org/forums/showflat.php/Number/6763535#Post6763535

I have yet to look at the agar plates but the liquid cultures have a ton of growth. For those of you that have done lc's of morels:
-Did you start with tissue or spore?
-What kind of growth and growth rate did you observe?


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Once the sin against God was the greatest sin; but God has died, and those sinners died with him. To sin against the earth is now the most dreadful sin, and to esteem the entrails of the unknowable higher than the meaning of the earth!

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Re: Morel liquid culture [Re: Ubermensch]
    #6769419 -

We need MorelMan! :smile:

Fabio

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Re: Morel liquid culture *DELETED* [Re: FreeSporePrints]
    #6773229 -

Post deleted by Morelman

Reason for deletion: Never again...



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Re: Morel liquid culture [Re: Morelman]
    #6773310 -

have you ever grown morels indoors?

or are you just in the process of trying?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Edited by Psychoslut (04/10/07 11:47 PM)

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Re: Morel liquid culture [Re: Morelman]
    #6773441 -

Yes the growth rate is impressive, if it is indeed morel myc. It is very thin and whispy, much like the black morel myc I had on agar last year. It eventually turned darker and thickened. Right now it looks as thin as cobweb mold. I've never seen or heard of that infecting lc's though.


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Once the sin against God was the greatest sin; but God has died, and those sinners died with him. To sin against the earth is now the most dreadful sin, and to esteem the entrails of the unknowable higher than the meaning of the earth!

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Re: Morel liquid culture [Re: Morelman]
    #6773501 -

Wow! a very brutal method like this doesn't harm the mycelium? Peroxide couldn't damage the mycelium?

Uber: Starting an LC from spores is strategically wrong..you can't know if those spores are contamined or not so you should first germinate them on agar media then inoculate the liquid culture. In truth you should check with a microscope, before inoculing the LC, if the culture contains bacterias. In a past thread about the microscopes in this section we all and exspecially RR discuss about this..exactly how bacteria appear under the lens :smile:

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Re: Morel liquid culture *DELETED* [Re: Ubermensch]
    #6773522 -

Post deleted by Morelman

Reason for deletion: Never again...



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Re: Morel liquid culture *DELETED* [Re: FreeSporePrints]
    #6773542 -

Post deleted by Morelman

Reason for deletion: Never again...



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Re: Morel liquid culture [Re: Morelman]
    #6773547 -

Thanks for the heads up Fabio. I know its a gamble, but I have some of the tissue on agar as well. Even though the growth is whispy, it is still clumped together like my lc's of other species and the surrounding liquid is clear.


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Once the sin against God was the greatest sin; but God has died, and those sinners died with him. To sin against the earth is now the most dreadful sin, and to esteem the entrails of the unknowable higher than the meaning of the earth!

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Re: Morel liquid culture [Re: Morelman]
    #6836010 -

Morelman said:
Yes, brutal.... but effective.

Healthy mycelium can survive peroxide. Contams cannot.



What is a good LC tek for morels?

Thanks in advance!


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"The only easy day was yesterday!"--Navy SEALs

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Re: Morel liquid culture [Re: The_Jackal]
    #6845978 -

Unrefined cane sugar seemed to work well. Around 5 grams per 200-250 mL of water.


--------------------
Once the sin against God was the greatest sin; but God has died, and those sinners died with him. To sin against the earth is now the most dreadful sin, and to esteem the entrails of the unknowable higher than the meaning of the earth!

Extras: Filter Print Post Top
Re: Morel liquid culture [Re: Ubermensch]
    #6848885 -

Ubermensch said:
Unrefined cane sugar seemed to work well. Around 5 grams per 200-250 mL of water.



Thanks! Did a dextrose, malt extract, activated charcoal, gypsum, LC tek ala monstermitch. Hope it works!


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"The only easy day was yesterday!"--Navy SEALs

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