Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures, Substrate Bags

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 8 hours, 6 minutes
Liquid and agar culture of H. ulmatius?
    #2698849 - 05/18/04 10:03 PM (19 years, 8 months ago)

I've got some peroxidated malt / B vitamin / low sodium soy sauce agar plates and some liquid culture of the same formula that were inocculated a week ago from an older malt agar plate of similar formula.

One of the dishes is showing vigorous growth, but the other two show no signs of growth at all. Two of the jars have something floating around in them, but I'm not sure if it is mycelium. The particles appear more point like rather than filiment like which I would expect mycelium to be. One of the liquid jars has definitely contaminated and there is a big grey blob growing on the side of the jar which started from a piece of the agar I used to inocculate it.

The mycelium had formed a solid mat on the origional malt agar plate. When I extracted the piece the mycelium mat tended to peeled up with the agar chunk.

My "plates" are wide mouth half pints, and I used a staple stuck into an exacto handle to extract the chunks. This exposed uncolonized agar which has since grown back in the origional jar seemingly from the mycelium that is climbing the sides of the jar rather than from the mycelium that is attatched to the agar.


My question is: "WTF"?


--------------------
Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ
(•_•)
<) )~  ANTIFA
/ \
\(•_•)
( (>    SUPER
/ \
(•_•)
<) )>    SOLDIERS
  / \


Extras: Filter Print Post Top
Offlineragadinks
MrBeatle
 User Gallery

Registered: 10/20/03
Posts: 1,298
Last seen: 3 months, 20 days
Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2699430 - 05/19/04 12:04 AM (19 years, 8 months ago)

Have you got a picture ?
And what kind of agar recipe is this you mentioned ?


Extras: Filter Print Post Top
OfflineBaby_Hitler
Errorist
 User Gallery

Folding@home Statistics
Registered: 03/06/02
Posts: 27,587
Loc: To the limit!
Last seen: 8 hours, 6 minutes
Re: Liquid and agar culture of H. ulmatius? [Re: ragadinks]
    #2699611 - 05/19/04 12:48 AM (19 years, 8 months ago)

The camera isn't good enough to catch much detail.

Here's a jar with the floaty stuff





Here's the one that is growing.





Here's the huge grey contam attatched to the side of one of the jars.





and here is a week old dish that hasn't changed since the day it was inocculated almost a week ago.



--------------------
Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ Ƹ̵̡Ӝ̵̨̄Ʒ
(•_•)
<) )~  ANTIFA
/ \
\(•_•)
( (>    SUPER
/ \
(•_•)
<) )>    SOLDIERS
  / \


Extras: Filter Print Post Top
OfflineMycena
mycoexplorer
Registered: 05/02/03
Posts: 270
Loc: indonesia
Last seen: 11 years, 9 months
Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2700073 - 05/19/04 06:15 AM (19 years, 8 months ago)

Peroxide will do that to many things - its shock
try moving it to a non peroxide plate or slant to recover

also your cultures have to be 100% clean to do liquid
try bottom innoculating then subbing off that to ensure its clean

its worth the hassle as one clean myc syringe can be stored easily while the plates have a short lifespan


Extras: Filter Print Post Top
OfflinecharlieXxXxX
Stranger

Registered: 03/29/04
Posts: 80
Loc: Earth, USA, WI, Madison
Last seen: 18 years, 11 months
Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2700327 - 05/19/04 08:41 AM (19 years, 8 months ago)


I've had cultures stall like your final picture and eventually
recover to fully colonize.

I believe Mycena is correct about the H2O2 shock. I bet the
culture in your second picture at least will fully colonize.


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures, Substrate Bags


Similar ThreadsPosterViewsRepliesLast post
* Help! Pure agar cultures needed. Some trades available! Basidiocarp 4,222 17 01/26/04 07:53 AM
by ChromeCrow
* R. Wayne's Peroxide Technique: Mold! Basidiocarp 3,061 11 02/24/05 12:29 PM
by debianlinux
* Making mycelium syringes from agar wedges. Baby_Hitler 5,453 9 06/04/03 12:52 PM
by Paid
* Standing water on agar YidakiMan 3,347 10 08/18/03 08:14 AM
by Suntzu
* Cultivating yeast cultures. Baby_Hitler 2,531 13 07/14/04 12:54 PM
by blackmold
* ATTN; anyone who has received Cordyceps cultures Suntzu 3,417 13 09/25/04 09:27 AM
by Corporal Kielbasa
* Culture Syringe wideweb 871 3 11/29/04 02:13 PM
by ragadinks
* Maintaining clean viable cultures for mass distribution? Baby_Hitler 1,203 1 09/27/04 03:19 AM
by ragadinks

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, Forrester, Stromrider, SHROOMSISAY01
1,541 topic views. 1 members, 3 guests and 8 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.017 seconds spending 0.004 seconds on 12 queries.