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OfflineBaby_Hitler
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Liquid and agar culture of H. ulmatius?
    #2698849 - 05/19/04 12:03 AM (12 years, 6 months ago)

I've got some peroxidated malt / B vitamin / low sodium soy sauce agar plates and some liquid culture of the same formula that were inocculated a week ago from an older malt agar plate of similar formula.

One of the dishes is showing vigorous growth, but the other two show no signs of growth at all. Two of the jars have something floating around in them, but I'm not sure if it is mycelium. The particles appear more point like rather than filiment like which I would expect mycelium to be. One of the liquid jars has definitely contaminated and there is a big grey blob growing on the side of the jar which started from a piece of the agar I used to inocculate it.

The mycelium had formed a solid mat on the origional malt agar plate. When I extracted the piece the mycelium mat tended to peeled up with the agar chunk.

My "plates" are wide mouth half pints, and I used a staple stuck into an exacto handle to extract the chunks. This exposed uncolonized agar which has since grown back in the origional jar seemingly from the mycelium that is climbing the sides of the jar rather than from the mycelium that is attatched to the agar.


My question is: "WTF"?


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Offlineragadinks
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Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2699430 - 05/19/04 02:04 AM (12 years, 6 months ago)

Have you got a picture ?
And what kind of agar recipe is this you mentioned ?


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OfflineBaby_Hitler
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Re: Liquid and agar culture of H. ulmatius? [Re: ragadinks]
    #2699611 - 05/19/04 02:48 AM (12 years, 6 months ago)

The camera isn't good enough to catch much detail.

Here's a jar with the floaty stuff





Here's the one that is growing.





Here's the huge grey contam attatched to the side of one of the jars.





and here is a week old dish that hasn't changed since the day it was inocculated almost a week ago.



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OfflineMycena
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Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2700073 - 05/19/04 08:15 AM (12 years, 6 months ago)

Peroxide will do that to many things - its shock
try moving it to a non peroxide plate or slant to recover

also your cultures have to be 100% clean to do liquid
try bottom innoculating then subbing off that to ensure its clean

its worth the hassle as one clean myc syringe can be stored easily while the plates have a short lifespan


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OfflinecharlieXxXxX
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Re: Liquid and agar culture of H. ulmatius? [Re: Baby_Hitler]
    #2700327 - 05/19/04 10:41 AM (12 years, 6 months ago)


I've had cultures stall like your final picture and eventually
recover to fully colonize.

I believe Mycena is correct about the H2O2 shock. I bet the
culture in your second picture at least will fully colonize.


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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