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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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LC contaminated? *pics* * 1
    #6533648 -

Hello, shroomerites! I've been reading everything I can on this site for a few months, and have decided to begin my adventure into mycological cultivation with the PF Tek, begun with a liquid culture (well, 2 actually). 1 was started 7 days ago with creamcorn's LC cloning tek (substituting 2 CC of Ecuadorian spores from a shroomery sponsor, of course) and 2 was started 5 days ago using Otto's LC tek, same spore syringe.

Oh, and both have been incubating in a TiT at ~78-80 degrees F for the entire time.

Anyway, here's some pictures of each of 'em. I can't tell if what I've got is contamination or mycelial growth.

Culture 1 before agitation (the dark blob is a pebble, added before steam sterilization)


Culture 1 after agitation


Another closeup of Culture 1 after agitation. It isn't really visible in any of these shots, but the grainy-looking whoknowswhat that's growing in there is very slightly beige colored, not pure white. Perhaps the mycelium is growing on some of the sediments?


Culture 2 before agitation. The dark, spidery thing is a couple of bent-up, mangled paperclips dropped in before sterilization. I use a regular 'fridge magnet to agitate it.


Culture 2, after agitation


Culture 2, after agitation. Again, a 2MP camera is not the best for this, but it's all I had. There's a great deal of sediment from the honey, and little clouds of white wispy-looking stuff (a few with dark centers) that stay on the bottom of the jar until agitated. Again though, none of the stuff is "white like the driven snow"


LC veterans, whaddya think? Bacteria, mold, mycelium, or Martian life?

edit: pics fixed

Edited by BoogieBot (02/06/07 06:49 PM)

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Re: LC contaminated? [Re: BoogieBot]
    #6537176 -

Only one of the photos is appearing.  The first one.  That brown sediment at the bottom does not look at all like mushroom mycelium to me.  :undecided:


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Re: LC contaminated? [Re: CureCat]
    #6539655 -

It may be possible that the paper clip is rusting and that is what is causing the yellow/orange color in that container. Would anyone second that?


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How to make your 50/50 casing 50/50+ with only eggshells:
http://www.shroomery.org/forums/showflat.php?Cat=0&Number=6519162&page=0&vc=#Post6519162

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Re: LC contaminated? [Re: Lun4e]
    #6540183 -

That makes sense. I agree.

I can now view all of the photos, and I say the top LC is pretty surely contaminated.


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Re: LC contaminated? [Re: CureCat]
    #6542573 -

I dont think paperclips rust???

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Re: LC contaminated? [Re: cpw1971]
    #6542725 -

I was considering whether they do or not myself..... Hmmm.


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Re: LC contaminated? [Re: CureCat]
    #6542746 -

Okay... Hehehehe, I found some amusing information. Paper clips do rust i guess.

Did a google search for "paper clips rust":
http://www.google.com/search?hl=en&q="paper+clips+rust"&btnG=Search

Quote:
Staples and paper clips rust, shedding black flakes that stain pages and mimic bug droppings.


http://www.hrc.utexas.edu/news/newsletters/2007/spring/archive.html

Edited by CureCat (02/08/07 01:35 AM)

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Re: LC contaminated? [Re: CureCat]
    #6542758 -

Ugh!  Stupid auto correct forum features!  I can't seem to ge the google link to work.  :mad2:


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Re: LC contaminated? [Re: CureCat]
    #6542985 -

oh ok :cool:  I have never seen a rusty one lol

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Re: LC contaminated? [Re: cpw1971]
    #6543985 -

They shed black flakes the resemble bug droppings, eh? That might explain the little black flakes that some of the mycelium has attached itself to.

I pitched culture #1 and have been soaking the jar, lid, and agitator in bleachwater for a few days. Jar #2 has REALLY taken off these past few days. Lots of white, fluffy stuff that looks like teeny, tiny clouds, some of it attached to little black flakes. I tried to get some pictures, but the 2mp camera just isn't doin' it. =\

Edited by BoogieBot (02/08/07 02:28 PM)

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Re: LC contaminated? [Re: BoogieBot]
    #6559386 -

Now that a few days have passed, what is the news on your cultures?

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