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OfflineXherrus
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Registered: 01/21/04
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Spread of contamination
    #2260221 - 01/21/04 05:18 PM (13 years, 8 months ago)

Aughhh!!
I prepared and inoculated 6 culture jars several days ago as per the PF Tek (firstly pre-sterilizing the vermiculite and rice flour in the oven) and they are growing splotches of cloudy stuff that looks very much like Cobweb Mold. One of them, which I had isolated from the rest earlier - since it was the first to grow cloudy blotches, I decided I'd better isolate it in case it wasn't mycelium - ended up growing a blue-green mold. When I noticed this I threw out the contents and rinsed the jar out with ammonia (I was out of Mold Killer spray).
Two others have been growing this cloudy stuff. One of them, with a spot about 2cm diam. has tiny black spots that look very suspect. The other's 1cm cloud is perfectly white. The others have shown no signs of growth.
I have isolated both jars from the other three and from each other.

My first question is, if this was caused by airborne bacteria, could this contamination have spread to the other jars, which I kept in a closet near to each other?

Secondly, I inoculated the jars with mycelium taken from rotting bark since I couldn't get a spore syringe - I assume this would be a very likely factor which caused the contamination. If so, is it possible that this contamination could have spread to other jars (which have a top layer of vermiculite)?

If I've missed something and you think I should RTFM, please refer me to the appropriate FM. All suggestions and thoughts are appreciated.

- Xherrus


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OfflineSuntzu
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Re: Spread of contamination [Re: Xherrus]
    #2260850 - 01/21/04 09:08 PM (13 years, 8 months ago)

Mycelium from rotting bark isn't usually the cleanest thing around. . .what species are you trying to propogate? If it's something that 'rotted bark' are you sure of the ID? Are you planning to go to dowels?
You can propogate mycelium from non-sterile sources, however you won't have much luck going to *sterile* media [aka PF/grain jars] without definitive clean-up. This is accomplished with agar/peroxide/antibiotic methods. To propogate something from a dirty source without this step, you would have to prepare a more selective substrate. For example, pasteurized woodchips/sawdust. This type of media is not as suitable for molds and such, though not immune to them. Always, the best way to go is to clean up a culture before trying to expand it.


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OfflineCultyVader
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Re: Spread of contamination [Re: Xherrus]
    #2261004 - 01/21/04 09:59 PM (13 years, 8 months ago)

You should not be getting mycelium from rotting bark. You are probably culturing some poisonious mushrooms. Get rid of everything and PM me. ill get you a print for christ's sake.


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OfflineXherrus
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Re: Spread of contamination [Re: CultyVader]
    #2261621 - 01/22/04 01:47 AM (13 years, 8 months ago)

Thanks for that info Suntzu.
Actually this is primo mycelium raided from a nature reserve - I went picking at this area last year when it was mushie season and there was nothing but these wicked sick Cubes in an area of several sqare metres. I would like to muck around with this stuff a bit since it's my first attempt at cultivation - thankyou anyway for the generous offer of the spore print, CultyVader.

As for the contamination issue, I discarded the 2 dirty cakes and 3 of the other 4 are growing snowy white mycelium already, not looking too bad so far and it's growing so FAST! Maybe cause I ground my own rice flour. But I won't "count my chickens before they hatch."

My main concern was that the contamination would spread from one jar to the others. Does this happen often (considering I have the layer of plain vermiculite on the top 1/2 inch of the jars as a buffer), and is it best to keep the jars separate during the mycelium's growth?


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OfflineMAIA
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Re: Spread of contamination [Re: Xherrus]
    #2262240 - 01/22/04 10:07 AM (13 years, 8 months ago)

Well, first you shouldn't put the floor in the oven, it will toast and destroy most of the nutrients.
Besides the facts others have pointed, are you sure it was cubensis mycelium ? Ps. Cubensis is not a wood lover, they are in nature mostly a coprophilic mushroom. I might add that, if you want to grow a mushroom which in nature grows on wood, then you should mimic nature by using a wood based substrate.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineXherrus
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Re: Spread of contamination [Re: MAIA]
    #2262306 - 01/22/04 10:43 AM (13 years, 8 months ago)

So "presterilization" destroys the nutrients? Dammit, I have totally lost faith in the Magic Mushroom Grower's Guide now... Do you think that the mycelium can still colonise the rice cakes? I mean someone must have used this technique before if it was in the MMGG, right?

Also now that you mention it I am not sure if they were P. Cubensis, however they were good mushrooms that looked very similar.

I really screwed up here huh. However I will keep on trying, the ingredients are very cheap and the culture jars and the mycelium were free. Thanks for the info ppl, I guess if all had worked perfectly I would be less knowledgeable and less able to deal with contamination and stuff.


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OfflineMAIA
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Re: Spread of contamination [Re: Xherrus]
    #2264978 - 01/23/04 07:56 AM (13 years, 8 months ago)

Don't loose your faith man, you're gaining experience and that's good. Get a syringe and play it safe using the good'old PF Tek.
Regarding your ongoing experiment, you could wait and see how it turns out but anyway, you could also be loosing some of your time.
Don't try building a house starting by the roof :wink: .....

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Offlinevermonster
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Re: Spread of contamination [Re: MAIA]
    #2268475 - 01/24/04 04:35 PM (13 years, 8 months ago)

Afoaf did the presterilization of the verm and
brf and at 20+ days all 6 jars are almost ready
to flip. The oven didn't seem to hurt the brf at
all.


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Offlineangryjslice
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Re: Spread of contamination [Re: vermonster]
    #2268776 - 01/24/04 06:39 PM (13 years, 8 months ago)

20+ days is a long time to wait for jars that are almost ready to flip. your brf probably lost much of is nutrients. i flip my jars after about 6 days.

~JSlice~


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Offlinevermonster
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Re: Spread of contamination [Re: angryjslice]
    #2271223 - 01/25/04 12:20 PM (13 years, 8 months ago)

Quote:

angryjslice said:
20+ days is a long time to wait for jars that are almost ready to flip.  your brf probably lost much of is nutrients.  i flip my jars after about 6 days.

~JSlice~




If thats the case, how does invitro cultivation work? :confused:


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Offlineangryjslice
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Re: Spread of contamination [Re: vermonster]
    #2271284 - 01/25/04 12:42 PM (13 years, 8 months ago)

i was refering to you losing nutrients by 'presterilizing' the brf. in my opinion, your grow is going slowly, probably due to the loss of nutrients by baking the brf.


~JSlice~


--------------------


Edited by angryjslice (01/25/04 12:43 PM)


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Offlinevermonster
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Re: Spread of contamination [Re: angryjslice]
    #2271333 - 01/25/04 01:00 PM (13 years, 8 months ago)

Quote:

angryjslice said:
i was refering to you losing nutrients by 'presterilizing' the brf. in my opinion, your grow is going slowly, probably due to the loss of nutrients by baking the brf.


~JSlice~




Ah, I got cha! Do you birth before seeing primordia? I'm
following the original PF-TEK that shows a jar at 23 days
so it seems I'm pretty close to normal timing.


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Offline284_27
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Registered: 07/13/03
Posts: 191
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Re: Spread of contamination [Re: MAIA]
    #2272037 - 01/25/04 05:21 PM (13 years, 8 months ago)

Quote:

MAIA said:
Don't try building a house starting by the roof :wink: .....

MAIA



I think that is the most important statement that I have heard in years . My brothers uncle in law figured he was pretty smart and went right to  grain . Becouse he is bull headed he got some sucess , but in the mean time wasted much time and energy . Sometimes it is better to crawl before you try to run .


--------------------
"I find that the harder I work, the more luck I seem to have."
- Thomas Jefferson

Fly high and I will meet you there .
- Timothy Leary


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OfflineXherrus
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Re: Spread of contamination [Re: 284_27]
    #2273827 - 01/26/04 06:47 AM (13 years, 8 months ago)

Ok every single jar I have tried has become contaminated with either blue-green shit or little black specks. Talk about the risk of innovating, the problem was I had no source for spores other than from the Botanic Gardens.

CultyVader or anyone else << If you are willing to give me a spore print/syringe I will send a money order for $10. I live in Australia so a print would be best to mail, right?

Or, if someone knows ANY online source for spores located in Australia please tell me, cause I've looked all over the friggin internet.


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OfflineAnnoA
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Re: Spread of contamination [Re: Xherrus]
    #2280738 - 01/28/04 02:26 PM (13 years, 8 months ago)

You should PM him.
Besides, there are several mushroom growers in australia on these boards, you should make a post in the marketplace, perhaps you have something to drade for a print or 2.


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OfflineAnnoA
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Re: Spread of contamination [Re: Xherrus]
    #2280741 - 01/28/04 02:27 PM (13 years, 8 months ago)



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