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Amazon Shop for: ½ Pint Jars, Rye Grain

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Offlinefirsttimer101
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Does most contamination occur early on?
    #3782837 - 02/15/05 11:56 AM (11 years, 9 months ago)

Just a quick question for all you experts, does most rye contamination occur early on? I inoculated my rye jars 5-6 days ago. The mycelium is growing very well. So far 7-8% of the jars are contaminated and I was wondering if I should expect many more. Does most of the contamination show up on the early side? Thanks guys, this forum has saved me quite a few times!


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OfflineMAIA
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Re: Does most contamination occur early on? [Re: firsttimer101]
    #3783444 - 02/15/05 02:54 PM (11 years, 9 months ago)

Contamination timings might depend on the kind of contaminant, temperature, humidity, etc. Therefore it's hard to tell when it will attack and how it'll spread.
If you should expect many more ? Probably. Any pics ?

MAIA


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Offlinefirsttimer101
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Re: Does most contamination occur early on? [Re: MAIA]
    #3784935 - 02/15/05 08:06 PM (11 years, 9 months ago)

I can't take any pics right now. From what I've read it's your average green mold, a penicillium I think. I was very careful with procedure..pressure cooking for 1 hour at 15-20Ibs, immediately transporting the jars to a closet with built-in shelves that had been cleaned out and washed down with bleach and Lysol, I tried to inoculate the jars as quickly as possible but this step is probably the weakest link as I'm a beginner and it takes some time to snag a piece of mycelium and transfer it...essentially I've done everything I can. I even spray down the jars with a fine mist of Lysol with water twice a day. The main problem is that I'm doing this in an environment that cannot be controlled completely. I want to eventually devote a room to growing and turn it into a super sterile mini-lab but for now I've just got a closet.


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OfflineMAIA
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Re: Does most contamination occur early on? [Re: firsttimer101]
    #3788127 - 02/16/05 06:06 AM (11 years, 9 months ago)

You're right about the inoculation process being the weakest link for a beginner, as it requires some skill.
Try building a glove box. You can read about it on the faq's.

MAIA


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Invisibleagar
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Re: Does most contamination occur early on? [Re: firsttimer101]
    #3791901 - 02/17/05 06:46 AM (11 years, 9 months ago)

>>>>I'm a beginner and it takes some time to snag a piece of mycelium and transfer it.<<<

I gather you used some colonized PF cake & made a transfer to a grain jar.

You might think about making a karo or light dry malt / dry detrose style liquid culture (LC)jar. Do a tiny transfer to it & incubate. If it's contaminated, that will usualy show up - along with myc growth. If its clean, use a syringe to innoc more grain jars. Lots less exposure to open air with LC innoc.


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Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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