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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD
#5407957 - 03/16/06 11:51 AM (17 years, 10 months ago) |
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It takes a thick healthy aggressive P. Cubensis strain LC.
A plastic microwave container, with tight fitting lid.
Drill, bit & Micropore tape.
Drill 3/8ths inch hole in tray lid & cover with Micropore tape.
Dehydrated compost, hydrated to field capacity with a liquid nutrient solution (LNS). (liquid nutrient solution is a concentrated compost tea)
Hydrate 30% (volume) vermiculite to just under field capacity with LNS. Hydrate 70% (volume) dehydrated compost with LNS, to field capacity. Combine both & mix well.
Fill plastic microwave tray with loamy / airy like mix & nuke twice for 3 minutes. With lid on tray, but only snapped down on one side. (allows steam to escape). Allow tray to rest inside the microwave 5 minutes. (otherwise heat/stream will cause lid will pop off, when you close it.)
Reach in microwave, snap lid shut, remove tray & cool overnight.
Following day, inoculate straight through tray lid with 20ml of thick LC. Cover inoculation hole with alcohol saturated Micropore tape. Then shake tray gently, to insure LC gets mixed well with substrate.
Incubate tray in darkness, at high room temperatures (72 to 78F) .
Once substrate is colonized, snap open lid & add a hydrated & pasteurized casing cover. (in this case 5 parts peat, 5 parts vermiculite, 1 part coir, pH balanced to 7.5 range).
Re-incubate tray (same as above), 3 days, or until mycelium crops out on the casing cover.
Expose tray to 12/12 off/on light cycle, FAE & room temperature. (72/76, 85 to 90% rh)
   
 First flush
 4th flush.
Substrate was my tailor made compost. This method failed with h/poo - repeatedly. (hell if I know why)
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: agar]
#5407967 - 03/16/06 11:54 AM (17 years, 10 months ago) |
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HAHA! That tray looks familiar. Chinese food anyone?
Nice job there. This should give the noobs something to feed on. I'm chowing.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: Delinquentes]
#5408012 - 03/16/06 12:07 PM (17 years, 10 months ago) |
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Yup, they are small test trays.
I tried this about 5 times, 3 trays each time. Until I got it down, right.
I also (stupidly) misfiled the in-between pic's somewhere. And, at this moment cannot find them.
This is not a thing for newbies, without the right ingredients.
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Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: agar]
#5408042 - 03/16/06 12:14 PM (17 years, 10 months ago) |
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>>Substrate was my tailor made compost. This method failed with h/poo - repeatedly.<<
Limited due to this, but interesting nonetheless.
-------------------- To find a place to live between the negatives and positives.
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Delinquentes
TIMMMAYYYYYYY


Registered: 02/07/06
Posts: 543
Loc: Hidin' in a bowl of rice
Last seen: 3 months, 16 days
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: Holydiver]
#5408081 - 03/16/06 12:21 PM (17 years, 10 months ago) |
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Compost has always interested me. Something about a pile getting to 150oF on its own intrigued me. It really cuts down on the disposal of yard and kitchen waste and its plain old fun to make. I'd love to have a tumbler, but have yet to get around to making one. A pile and a pitch fork works wonders though.
-------------------- "The price of meat has just gone up, and your old lady has just gone down!" ~~ Zappa Click Here for a ghetto ozone generator Tek. Want to learn more about ozone as a bacteria killer? Click Here
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campinman
Fun-Guy


Registered: 09/01/05
Posts: 398
Loc: SoOn
Last seen: 7 years, 3 months
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: agar]
#5408476 - 03/16/06 01:36 PM (17 years, 10 months ago) |
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First, sweet tek man, once again, my hats off to ya my friend, on one hand you should write a big book of all these teks and tricks, however, on the other hand, i like getting these for free 
quick question in regards to: Quote:
liquid nutrient solution (LNS). (liquid nutrient solution is a concentrated compost tea)
this.
this LNS wouldnt be the liquid poo runoff from a horse farm, would it? i have a friend who thinks the liquid poo(for lack of a better term) will do wonders, however i think the liquid poo is just the garbage that leaches out of the poo
how would i go about making some of your LNC compost tea?
-------------------- No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction. "I wish my lawn was emo, so it would cut itself."
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: campinman]
#5408537 - 03/16/06 01:46 PM (17 years, 10 months ago) |
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shouldn't the micro spore tape be over the hole even during the nuking? maybe i just misread that. interesting post as always agar
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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ke1n
Stranger


Registered: 11/15/05
Posts: 359
Last seen: 14 years, 6 months
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: skeletor]
#5408566 - 03/16/06 01:53 PM (17 years, 10 months ago) |
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skeletor i dont think so, while nuking/PCing anything you want a place where steam can escape or BOOM
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Everything that is posted, including pictures and text, are a result of fictional storytelling using images found online and/or created using the latest graphics software. I am a fictional writer who likes to explore the internet world. ------------------------------------ http://www.adobe.com/
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cappa
Nerd
Registered: 02/12/06
Posts: 854
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: ke1n]
#5408616 - 03/16/06 02:08 PM (17 years, 10 months ago) |
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Perhaps the poorly broken down ammonia nitrate kills the myc in the h/poo tests? In compost it gets broken down and 'assimilated' alot more, correct? The nitrogen is freed, and the ammonia gets broken down and distributed to newly formed, healthy(for mushrooms) compounds.
Whatcha think?
-------------------- Their are 10 types of people. Those that understand binary, and those who don't. ~Cappa.
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skeletor
the dude
Registered: 09/06/05
Posts: 1,235
Loc: heaven on earth
Last seen: 17 years, 4 months
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: cappa]
#5408774 - 03/16/06 02:50 PM (17 years, 10 months ago) |
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but thast why he had the lid cracked. if you snap on the lid after 5 mins and let it cool you still have a hole. i think at that point the micro tape would go on. or else there is no point in putting a lid on beucase lid with a hole isn't going to keep out contams while it cools overnight right. anyhoo im sure its just a wording thing.
ahh see i just reread the original post. what it said what inoculate right through the lid then put tape over that. there was never any mention of tape over the hole that was drilled into the lid that was just implied. confusion solved
-------------------- im sorry about your mother. She was a terrible attrative woman. Get back to nature; hunting burgers and gathering fries.
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mogur
regnartS

Registered: 11/15/05
Posts: 322
Loc: Puget Sound
Last seen: 11 years, 2 months
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: skeletor]
#5408826 - 03/16/06 03:01 PM (17 years, 10 months ago) |
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Quote:
skeletor said:... there was never any mention of tape over the hole that was drilled into the lid that was just implied. confusion solved
The fourth line down.
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eatyualive
Eat's You Alive :)


Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: PREVEIW - NO GRAIN SPAWN OR PC BULK METHOD [Re: mogur]
#7480035 - 10/03/07 08:19 AM (16 years, 3 months ago) |
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lol those trays i get from the chinese food place. they are awesome and free with your order. can't beat that...
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