Is there a correct procedure?

Home | Community | Message Board

You are not signed in.
New Account

Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop:

Isolated Cubensis Liquid Culture For Sale

High THC Strains

North Spore Mushroom Grow Kits & Cultivation Supplies

All-In-One Bags That Don't Suck

Jump to first unread post

Pages: 1

Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.

Xochipili
journeyman

Registered: 01/05/02
Posts: 68

Is there a correct procedure?

#540342 - 02/04/02 02:22 PM (24 years, 6 months ago)

I mean exact details, not just turn your head and dont breathe. a friend needs to make new G1 spawn masters. to do this he's got to transfer the neglected plates that were used for strain isolation #1 to new plates. 3 out of 10 plates have nasty mold growing on them(description follows). I cant get a great description but they appear to be one black and other green. Neither mold is growing concentrically, the way penicillin looks or even aspergillus. the black one is a solid black color growing all around perfectly snow white thick mycelial strands. the other doesnt bother me quite as much, its a green or two shades of green on different plates. How can these be most safely disposed of. they are in plastic bags, but the plates are glass, and need to be saved. the mold cultures look a bit powdery, like they've sporulated. its difficult to choose the best way, even with the excellent desciptions in TMC of fungi imperfecti. it could be several including aspergillus, green or black form (aspergillus niger) which is deadly. thanks for answers.

--------------------

Extras:

Top

Anonymous

Re: Is there a correct procedure?

[Re: Xochipili]
#540415 - 02/04/02 03:53 PM (24 years, 6 months ago)

Sterilize the contaminated plates immediately, then discard all contents, clean plates very well in a solution of bleach and water.

I think that was the only question you had, probably could have left out the beggining part of your post. Just in case it wasn't, pick the cleanest and most uniform of the original transfer plates, and do new transfers with that. If you got three contaminated out of ten, you probably should do another ten to be safe.