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Offlinekingstroke
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p.mexicana on agar - is this the correct procedure?
    #3206185 - 10/02/04 11:12 AM (12 years, 3 months ago)

ok here is what I plan to do:

I have 250 ml malt agar on bottle, I follow the procedure on the bottle and puts it into boiling water so it turns liquid.

I pour this over into petri dish(es) wich I have sterilized in the pressure cooker. I have one print of the specie I want to grow (Psilocybe Mexicana) Is one print enough for lets say 10 petri dishes? If I only scrape a little in each?

Should I just take the spores directly on the agar when its cooled down? or should I put them in before the agar turns more gel like again?

Then I wrap the petri dishes in plastic wrap and put them in a petridish rack. Should I just put this rack into the incubater (tub in tub incubator) in same conditions as I have my jars?


Edited by kingstroke (10/02/04 11:13 AM)


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InvisiblePrisoner#1M
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Re: p.mexicana on agar - is this the correct procedure? [Re: kingstroke]
    #3206204 - 10/02/04 11:26 AM (12 years, 3 months ago)

Quote:

kingstroke said:
I follow the procedure on the bottle and puts it into boiling water so it turns liquid.




it puts the lotion on its skin or else it gets....

you need to sterilize the media after it's combined, add it to cold water
and pressure cook it for 15-20 minutes

Quote:

I pour this over into petri dish(es) wich I have sterilized in the pressure cooker. I have one print of the specie I want to grow (Psilocybe Mexicana) Is one print enough for lets say 10 petri dishes? If I only scrape a little in each?




if you have Polycarbonage or glass petris then you can sterilize them, if they
are polystyrene then they will melt... it should say in the package what type
they are

you can innoculate 20-30 petris with a small spore print (a 1" print)
wait for the agar to cool and gel before putting the spores on the agar
use glad cling wrap for for its breathability and then you can incubate
it just as you would your jars


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Offlinekingstroke
#gcc -o fungi.c

Registered: 09/29/04
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Re: p.mexicana on agar - is this the correct procedure? [Re: kingstroke]
    #3209232 - 10/03/04 09:05 AM (12 years, 3 months ago)

I have glass dishes :smile:

The bottles say they are sterile and ready for use on sterile petri dishes. A friend working on some lab got them for me.


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Offlinediscman1
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Re: p.mexicana on agar - is this the correct procedure? [Re: kingstroke]
    #3209246 - 10/03/04 09:18 AM (12 years, 3 months ago)

The petri dishes may be sterile, but your agar isn't if it has been exposed to the air.


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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