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Amazon Shop for: Agar, Petri Dish

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Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 3 years, 1 month
Agar & Peroxide Question
    #3816983 - 02/22/05 03:26 PM (11 years, 9 months ago)

I was trying to follow the peroxide tek bit they but they dont have a ratio for peroxide to petri dish basis. I did a search and it wasnt there either. Anyone on the board with skills in the agar department know what a good ration for peroxide to agar in a petri dish would be. I have a 350 ml Bottle of RTP Nutrient agar and I now for a fact Im not going to use the whole entire bottle in one run so I think it would be a waste of time to peroxidise the whole damn bottle. Any suggestions??

-Odd :confused:


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??


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Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 3 years, 1 month
Re: Agar & Peroxide Question [Re: oddiseus]
    #3817651 - 02/22/05 05:56 PM (11 years, 9 months ago)

Bump -Sorry- Just need some help.


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
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Re: Agar & Peroxide Question [Re: oddiseus]
    #3817673 - 02/22/05 06:01 PM (11 years, 9 months ago)

first, are you starting from spores or culture


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Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 3 years, 1 month
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817691 - 02/22/05 06:05 PM (11 years, 9 months ago)

Im gonna start from a culture. Thats the same as using tissue from a freshy right??


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??


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InvisiblePrisoner#1M
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Re: Agar & Peroxide Question [Re: oddiseus]
    #3817708 - 02/22/05 06:08 PM (11 years, 9 months ago)

yes, use 1.5 mL of peroxide, it's worked for me with agar wedges


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Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 3 years, 1 month
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817715 - 02/22/05 06:10 PM (11 years, 9 months ago)

should I just squirt it into the petri dish right after I pour the agar in?? and give a lil shakey shakey.?


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 191,505
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Re: Agar & Peroxide Question [Re: oddiseus]
    #3817740 - 02/22/05 06:15 PM (11 years, 9 months ago)

wait for your agar to cool to about 150F-160F and squirt it into the the agar,
give it a swirl to mix it in after each squirt and make sure you recap it first


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Offlineoddiseus
The PsychofunkFirefly

Registered: 10/05/04
Posts: 456
Loc: Doldrums & Faultlines
Last seen: 3 years, 1 month
Re: Agar & Peroxide Question [Re: Prisoner#1]
    #3817778 - 02/22/05 06:25 PM (11 years, 9 months ago)

Is their anyway you can eyeball the temp? I dont have anyway of measuring that. If I get a thermometer what kind should I get? Lab Grade?
Thanks for your help by the way..

And one other thing I can't believe you know who goatse boy is. That is hilarious.

-Odd


--------------------
Though you can't go back and make a new beginning. You can start today to make a new end.

Traveller, there is no path, paths are made by walking

If Jesus was jewish...Why does he have a mexican name??


Post Extras: Print Post  Remind Me! Notify Moderator
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Amazon Shop for: Agar, Petri Dish

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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