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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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inoculation procedures, in open air.
#5943734 - 08/08/06 11:12 AM (17 years, 5 months ago) |
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If your syringe, inoculate & jar content is clean?
It's not tough to learn to inoculate in poor conditions, with a little planning.
A small room is preferable. (Like a bathroom)
Still air is preferable.
Sanitized still air (oust sprayed high, and allowed to settle) is better yet.
Wearing a face mask is preferable. (You have to breath, and your breath is not clean).
Some sort of hair covering is preferable.
Wearing freshly laundered clothing is preferable.
Sanitizing your hands, up past your elbows is preferable.
A hard surface counter, or table top, micropore tape, a few paper towels, and a bottle of 70% alcohol is all it takes.
Situate your jars, so you can reach each one with minimal movement.
Wipe down the counter top with alcohol saturated paper towels, to sanitize it.
Wipe down all the jars, the same way, especially the lid area.
Alcohol soak a few more paper towels.
Pull sheath off needle, surround needle in alcohol saturated paper towel. (Saturated means damp, not dripping wet)
Pick the first jar up, and swab down lid again, with another alcohol saturated paper towel.
Leave that saturated paper towel, in place over the lid inoculation site.
Pick up syringe, with needle still wrapped in alcohol saturated paper towel, and position needle over inoculation site of the jar in front of you.
Slide saturated paper towel off inoculation site.
Press needle against inoculation site, with needle still wrapped in saturated paper towel.
Push needle into the jar, with saturated paper towel held in place around syringe.
INJECT JAR
Withdrawn needle being careful to keep it wrapped in saturated paper towel, as you withdraw it.
Set the syringe down in front of you, being careful to keep the needle wrapped in saturated paper towel.
Cover injection hole with alcohol saturated piece of micropore tape.
Pick up the next jar, swab down the lid & repeat process above.
The object of all this, is to minimize open air exposure of the needle, and inoculation site during the injection procedure.
Once you are comfortable with the process.
While not preferable, it can be done in open air, on a kitchen counter top.
Edited by Tippinthru (08/08/06 11:28 AM)
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canadarocks
Stranger
Registered: 05/29/05
Posts: 15
Last seen: 15 years, 3 months
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Re: inoculation procedures, in open air. [Re: Tippinthru]
#5943810 - 08/08/06 11:33 AM (17 years, 5 months ago) |
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^ *shrug* I've always just done it in "the open air". I use similar precautions but if planned correctly, I agree - there is little chance of contamination.
I just use jar of honeywater/myc with micropore tape on hole in lid. My cakes all have foil on top, so I simply draw the syringe full from the honeywater/myc jar, then poke a hole with the needle to inoculate through the aluminum foil 4x per cake. I use a sterile needle/syringe each time of course. But I haven't had any cakes lost to contams using this process.
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Omnicracker
Crusted Trolltivator

Registered: 09/18/05
Posts: 1,421
Loc: Wal-Merica
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Re: inoculation procedures, in open air. [Re: canadarocks]
#5943976 - 08/08/06 12:19 PM (17 years, 5 months ago) |
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Open air all the way! unless youre using agar or g2g, its not hard to have a 0% contam rate from an open air inoculation.
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royer
±±±±±±±±±±

Registered: 05/15/06
Posts: 4,801
Loc: anywhere but here
Last seen: 4 years, 10 months
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Re: inoculation procedures, in open air. [Re: Omnicracker]
#5944006 - 08/08/06 12:28 PM (17 years, 5 months ago) |
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all of mine are done in open air with windows open and i sanatize a little but no mask and hair net. there is no hurricane out side or anything so there is not to much of a breeze. i only use a glove box for agar. i make my syringes the same way in open air . i have no prob with contam.
-------------------- ================================================= if you have any questions please feel free to pm me , thx :-)
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: inoculation procedures, in open air. [Re: royer]
#5944107 - 08/08/06 01:05 PM (17 years, 5 months ago) |
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Quote:
royer9864 said: all of mine are done in open air with windows open and i sanatize a little but no mask and hair net. there is no hurricane out side or anything so there is not to much of a breeze. i only use a glove box for agar. i make my syringes the same way in open air . i have no prob with contam.
Odds are, the longer you gamble, the more likely you are to lose money. That is a proven statistical fact.
The same holds true for inoculation procedures.
Using every available precaution, to maintain sanitary inoculation procedures decreases the odds of contamination.
Simply stated, the cleaner, the better.
That is a proven statistical fact.
Many can do open air inoculation repeatedly, with a very low contamination rate.
With open air inoculations, statistically, somewhere in the future, for any number of possible reasons, some circumstance will change slightly for a moment, and the contamination rate can increase dramatically.
It is simple common sense, to take every possible precaution to insure success. That holds true in all area's of life.
I have an acquaintance, who is now terminally ill with AIDS, because she did not take simple precautions. The consequences of which, is now a death sentence.
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: inoculation procedures, in open air. [Re: Tippinthru]
#5944289 - 08/08/06 02:07 PM (17 years, 5 months ago) |
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Quote:
Tippinthru said: With open air inoculations, statistically, somewhere in the future, for any number of possible reasons, some circumstance will change slightly for a moment, and the contamination rate can increase dramatically.
It is simple common sense, to take every possible precaution to insure success.
True.
I do open air inoculations on the kitchen counter. I have had issues in the past, but working with LC it's a godsend. My contam rate is less than 1%
These are risks i am willing to take for convience sake, as well as making more spawn than i know what to do with.
but these are just what works IME, I reccomend everyone to work with extreme caution to increase chances of a good grow.
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: inoculation procedures, in open air. [Re: Oatman2000]
#5944517 - 08/08/06 03:30 PM (17 years, 5 months ago) |
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 I am guilty of the "more than you know what to do with" syndrome myself.
Once I mastered LC's, I made FAR more than I need.
Having so many LC's all healthy and mentally shouting out to me.
WE WANT GRAIN, WE WANT GRAIN..... 
I have made FAR more wbs/grain spawn than I have room to use.
The problem being, grain spawn is at optimal vigor, when fully colonized, and for about 2 weeks more.
IMO, it begins to lose "vigor", after that.
So, it goes downhill, the older it gets.
Which is not a good thing.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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Oatman2000
-=Outa Space=-



Registered: 05/10/05
Posts: 2,877
Loc: Planetary Nebula
Last seen: 1 year, 4 months
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Re: inoculation procedures, in open air. [Re: Tippinthru]
#5944546 - 08/08/06 03:39 PM (17 years, 5 months ago) |
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but wasn't there discussion about the longer grain colonizes, the more potent the fruits become?
--------------------
Spawning to COIR
My Chocolate Recipe
WBS QUART SPAWN JAR PREPERATION ----------------------------
4-PO-DMT; 4-phosphoryloxy-N,N-dimethltryptamine
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Tippinthru
contented

Registered: 04/07/05
Posts: 1,131
Loc: "The Garden"...
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Re: inoculation procedures, in open air. [Re: Oatman2000]
#5944615 - 08/08/06 04:00 PM (17 years, 5 months ago) |
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I would think you mean as substrate.
Even there, there is a point of over-colonization.
Which can also become problematical.
-------------------- Perfection is attained by slow degrees; it requires the hand of time... [
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