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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood

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Offlinesillvyr
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Hydrogen Peroxide, will it kill mycelia?
    #534031 - 01/28/02 03:39 PM (15 years, 27 days ago)

Hey Y'all,

I cut out a mold-infected piece of substrate from the only jar I had with yuckiness in it. I did this the same way I inoculated the jars, in my poor man's laminar flow hood (my oven w/rack pulled out @ 170 degrees). I sterilized the "digging spoon", I wore a mask and was myself very clean. I then poured a small amount of hydrogen peroxide (around 4-5 cc's) in the jar and swished it around the site I removed the substrate from. My question is: will the H2O2 kill mycelia? Does H2O2 kill contaminants effectively?
here is the contaminant before it was removed:

just so you guys can see what I'm dealing with. Hope I didn't just kill mycelia!
Thank you all for reading and posting, I've learned a lot, and so much more to learn!


--------------------
The "Little Mushrooms" are but emisaries to the Overmind.....



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Invisiblephrozendata
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: sillvyr]
    #534064 - 01/28/02 04:19 PM (15 years, 27 days ago)

No, H202 will not kill the mycelia. It may damage it a little but it'll recover.

It depends on the bacteria or mold -- it may or may not kill it.


--------------------
"There's only one corner of the universe you can be certain of improving and that's your own self. So you have to begin there, not
outside, not on other people" - Aldous Huxley


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OfflineXAZIA
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: sillvyr]
    #534066 - 01/28/02 04:20 PM (15 years, 27 days ago)



--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."

Bob Marley

Remember, EGOISM is the beginning, the source, and the root of EVIL!

http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html


Edited by XAZIA (01/29/02 03:06 PM)


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InvisibleRoadkill
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: XAZIA]
    #534138 - 01/28/02 05:28 PM (15 years, 27 days ago)

XAZIA,

Your very sharp....i was wondering too if he had diluted it.
I hope he did.
:wink:
 


--------------------
Laterz, Road

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You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


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InvisibleZen Peddler
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: sillvyr]
    #534154 - 01/28/02 05:49 PM (15 years, 27 days ago)

diluted right it should not kill mycelia but may kill the unwanted nasties' spores before they have a chance to take hold.


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Offlinesillvyr
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: Zen Peddler]
    #534932 - 01/29/02 01:36 PM (15 years, 26 days ago)

Hi all,

well, the peroxide did not kill the mycelia, but it didn't kill the mold, either. I am back at square one! I want to try to save the mycelia that are still good--today they are showing strong rhizomorphic growth and is on the other side of the cake (so about 2 inches of uncolonized substrate separates them). The mycelia have grown considerably since yesterday, also. I think that if I can get them out of the jar that they would fare better. I have a few jars that are not colonizing, so I think it makes sense to get the healthy mycelia out, clean the uncolonized jars, and try to inoculate those jars with live culture. At the very least it will be an experiment, I don't really have anything to lose. Any suggestions on accomplishing this?


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The "Little Mushrooms" are but emisaries to the Overmind.....



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OfflineAnnoA
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: sillvyr]
    #534973 - 01/29/02 02:24 PM (15 years, 26 days ago)

Dump the contaminated jar(s) and refine your sterile technique.


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OfflineXAZIA
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Registered: 08/22/01
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Re: Hydrogen Peroxide, will it kill mycelia? [Re: Anno]
    #534994 - 01/29/02 03:11 PM (15 years, 26 days ago)

i agree with anno (of course). if your cake is contaminated with a nasty that is unaffected by the hydrogen peroxide i would not venture further down that road. chuck it and start over. better to be safe than sorry.


--------------------
"Emancipate yourself from mental slavery, none but ourselves can free our minds."

Bob Marley

Remember, EGOISM is the beginning, the source, and the root of EVIL!

http://www.fanaticus.com/pf-tek.htm
http://www.mycotopia.net/teks/hongus.html


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Offlinesillvyr
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Registered: 01/12/02
Posts: 125
Loc: under a tree
Last seen: 8 years, 7 months
Re: Hydrogen Peroxide, will it kill mycelia? [Re: XAZIA]
    #535066 - 01/29/02 04:51 PM (15 years, 26 days ago)

OK Anno & Xazia, I'm following your advice. Thank you both very much!


--------------------
The "Little Mushrooms" are but emisaries to the Overmind.....



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Amazon Shop for: ½ Pint Jars, Laminar Flow Hood

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