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Leukoism
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Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Hydrogen Peroxide?
#3184970 - 09/27/04 01:32 AM (20 years, 2 months ago) |
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Ok, I have been trying to figure out how to use Hydrogen Peroxide to simplify my preperations for clean mushroom environment. My question is how exactly do i do this? How much am I suppost to add? Is there a different procedure than the PK-Tek? I really want to find out because it would definatly make it easier to grow. There would be no need for all the cleanliness procedures because the hydrogen peroxide kills the mold and bad contaminates but allows the mushrooms to grow efficiantly and I guess the mycentium converts the hydrogen to oxegen or somthing like that. Please, any info is good info.
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Lifenergy
Yo Mama
Registered: 08/05/04
Posts: 766
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3184977 - 09/27/04 01:34 AM (20 years, 2 months ago) |
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The amount is generally 90% water, 10% h2o2.
-------------------- Everything that we see is a shadow cast by that which we do not see.
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3184978 - 09/27/04 01:34 AM (20 years, 2 months ago) |
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one tea spoon of h2o2 to every 15 water!
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Leukoism
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Registered: 09/18/04
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Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: liveby]
#3184981 - 09/27/04 01:37 AM (20 years, 2 months ago) |
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Does this method work? Is it true that I wont need to pressure cook or do all the normal cleanliness procedures. Just add Hydrogen and grow mushrooms?
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 8 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3184989 - 09/27/04 01:39 AM (20 years, 2 months ago) |
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you make absolutely no sense. how are you going to use the hydrogen peroxide?
Is it true that I wont need to pressure cook or do all the normal cleanliness procedures. -ALWAYS be as clean as possible. If you don't do this, you're setting yourself up for failure.
Just add Hydrogen and grow mushrooms? -add hydrogen to what? water? how's the water going to be used?
this post makes no sense. clarify.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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liveby
Wasted For Time
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Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3184999 - 09/27/04 01:42 AM (20 years, 2 months ago) |
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i used HP to sarilize pasta ,but yeah !
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Leukoism
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Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: ZeroArmy27]
#3185013 - 09/27/04 01:48 AM (20 years, 2 months ago) |
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I read that the hydrogen kills all the contaminates, so if thats the case I wouldnt need to pressure cook. Thats why I wrote this post for, its becuase I have no clue how to use the hydrogen peroxide or what to do. I want to find out if what I read was true and if It actually does these things
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atomic1
enthusiast
Registered: 09/18/03
Posts: 1,123
Loc: Appalachia
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185018 - 09/27/04 01:52 AM (20 years, 2 months ago) |
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H2O2 is only a helpful tool in fighting off contams and by no means a substitute for any normal cleanly procedures or a pressure cooker.
H2O2 kills spores not contaminates and also breaks down easily from what i understand so its not like it lasts. A misting solution of like a 10:1 water/h202 ratio is good to use on casings to ward off future contams before their spores can germinate.
Edited by atomic1 (09/27/04 01:56 AM)
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185019 - 09/27/04 01:53 AM (20 years, 2 months ago) |
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i wrote a teck on how to sterilize only using HP and boiling in the advanced culti section a while back!
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185020 - 09/27/04 01:54 AM (20 years, 2 months ago) |
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i would say no, hydrogen peroxide won't kill all of the contaminants. Where did you read that at? (link please).
it seems liveby has done this with pasta. hrm. can you post a tek or did you follow one? (link, if tek was followed, please)
if you're doing the PF jar method, you don't HAVE to pressure cook, you can boil the jars... i did that for my first grow and only got one contamed jar.
this is the strangest thing i've heard in a while.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185025 - 09/27/04 01:56 AM (20 years, 2 months ago) |
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of course it diff in the medium used , pf needs to be pc or frac sterizied , also my tek has yet to be further explored!
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Cornelius
Heinous Anus
Registered: 02/12/04
Posts: 694
Loc: T.O.
Last seen: 10 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185026 - 09/27/04 01:57 AM (20 years, 2 months ago) |
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you've been reading wrong, or not thoroughly enough. H202 can be used in many ways to help keep operations more sterile, but it's just an extra procedure, and an added safety net. It is by no means used as a substitute for pressure cooking or any of the many other sterility methods you'll read and use when you go back and re-read the FAQ's a LOT more thoroughly...
Good Luck!
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Cornelius]
#3185030 - 09/27/04 01:59 AM (20 years, 2 months ago) |
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Leukoism
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Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: liveby]
#3185034 - 09/27/04 02:00 AM (20 years, 2 months ago) |
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I found it here http://members.aol.com/PeroxyMan/
I also found the information about the Hydrogen Peroxide method here http://www.shroomery.org/index/par/23462
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liveby
Wasted For Time
Registered: 06/15/04
Posts: 1,511
Last seen: 13 years, 7 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185036 - 09/27/04 02:02 AM (20 years, 2 months ago) |
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Quote:
Leukoism said: I found it here http://members.aol.com/PeroxyMan/
I also found the information about the Hydrogen Peroxide method here http://www.shroomery.org/index/par/23462
yeah , the first link is a book , second is agar
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 8 months
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Re: Hydrogen Peroxide? [Re: liveby]
#3185037 - 09/27/04 02:02 AM (20 years, 2 months ago) |
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uh, i'd say it wasn't the hydrogen peroxide that got rid of the contams, but the boiling. maybe the hydrogen peroxide could be considered an additive, but since h2o2 breaks up at 120 deg. F, i don't see how your tek would help.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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Leukoism
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Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: ZeroArmy27]
#3185039 - 09/27/04 02:06 AM (20 years, 2 months ago) |
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Whats the difference between the two?
Quote from the webpage
"So why use hydrogen peroxide in mushroom growing? Hydrogen peroxide simplifies the whole process of growing fungi. There's no need to build a sterile laboratory, buy a special pressure cooker, or even construct a glove box. A low concentration of peroxide keeps out the contaminants, while allowing healthy growth of mushroom tissue."
It sounds like they have some kind of solution, is it all fake?
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 8 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185040 - 09/27/04 02:10 AM (20 years, 2 months ago) |
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i don't know, i've never read the book and tried the method. if you want to do that, by all means, try it. otherwise, i'd say shy away from that.
laziness and misinformation are the cause of most failures in this hobby IMO.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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atomic1
enthusiast
Registered: 09/18/03
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Loc: Appalachia
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185059 - 09/27/04 02:23 AM (20 years, 2 months ago) |
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Are u doing pf tek or grains?
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Leukoism
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Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: atomic1]
#3185061 - 09/27/04 02:25 AM (20 years, 2 months ago) |
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PF-Tek
-------------------- May it have been the one that you are no longer aware, but now it has become a reality.
-----Enter the craze untill you have seen!-----
--------------------------
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Leukoism
Dark Canopy View
Registered: 09/18/04
Posts: 272
Last seen: 19 years, 6 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185097 - 09/27/04 02:54 AM (20 years, 2 months ago) |
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So is the peroxide only effective after the mycentium is devoloped?
-------------------- May it have been the one that you are no longer aware, but now it has become a reality.
-----Enter the craze untill you have seen!-----
--------------------------
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Spudz76
Got mycelium on the brain...
Registered: 02/08/04
Posts: 391
Last seen: 14 years, 8 months
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185183 - 09/27/04 03:33 AM (20 years, 2 months ago) |
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Yes. You cannot use peroxide with spores, it kills them. You can try an all-peroxide method (just use peroxide mixed in with all water used in whatever tek you follow) but you have to make a non-peroxide karo jar or some other liquid culture so you can inoculate with clean, live mycelium. You will not likely enjoy the ratio of contam to noncontam though in most all opinions. It 'works', just not guaranteeably very well. Some materials will break down the peroxide well before it can do any good, also - but it's sort of hard to figure out what does and what doesn't (though it's related to enzymes AFAIK).
-------------------- Anywhere in this posting where I have referred to myself or my possessions, it is a personalization of events and items experienced or possessed by someone else. I do not personally do anything illegal, and I hope nobody else does either.
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ZeroArmy27
I didn't go to work for a month.
Registered: 08/31/04
Posts: 1,169
Loc: Middle of nowhere
Last seen: 6 years, 8 months
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Re: Hydrogen Peroxide? [Re: Spudz76]
#3185386 - 09/27/04 05:49 AM (20 years, 2 months ago) |
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basically, do it the right way. i don't think many people would readily rely on that "peroxide tek." it's just too vague, and it doesn't really seem to cover all of the bases.
-------------------- "a monkey would fuck you up if you tried to put it in a autoclave" - Psychoslut
"it's not like the admins and mods are a tight-knit group of hippies that spend their life together in a log cabin tie-dying shirts and stringing beads inbetween bonghits." - Wiccan_Seeker
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Silven
Registered: 08/30/04
Posts: 2,072
Loc: El Mexicano
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Re: Hydrogen Peroxide? [Re: ZeroArmy27]
#3185439 - 09/27/04 06:38 AM (20 years, 2 months ago) |
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I agree, PC'ing still sounds like the best bet. Even boiling and fractional sterilizing sounds like it would be more effective than just using H2O2.
If this is your first grow I'd suggest PF-Tek For Simple Minds in our Mushroom Cultivation section. This is what I used. Very thorough.
- Silven
-------------------- What do you bring to the table?
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KaptKid
Spaced Pirate
Registered: 12/11/03
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3185509 - 09/27/04 07:34 AM (20 years, 2 months ago) |
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If you want it sterilized you need a PC. PERIOD.
-------------------- Child of the 60's, Tripping ever since.
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slammin007
yearnin' talearn
Registered: 10/04/03
Posts: 181
Last seen: 18 years, 1 day
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Re: Hydrogen Peroxide? [Re: Leukoism]
#3186761 - 09/27/04 02:30 PM (20 years, 2 months ago) |
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seems like everyone would be doing it if it worked that well, don't you think? I mean, who wants to take all those precautionary measures.....
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scatmanrav
Brainy Smurf
Registered: 05/08/04
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Re: Hydrogen Peroxide? [Re: slammin007]
#3187295 - 09/27/04 04:40 PM (20 years, 2 months ago) |
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Hydrogen Peroxide will certainly work in growing mushrooms as a substitute for PCing. It will kill everything just fine. The problem is it won't work with grains, it's meant for wood loving species. Grains have organic enzymes that break down the H202 (like mycelium does). There is a mod to the tek using some grains but more H202 has to be used...
H202 is a great additive in all water going into your chamber but you really dont need much in misters/dunks/humidifiers. I use it in everything. Safe for myc (im smaller amounts but large amounts of H202 will kill myc the same as it will kill molds in large amounts) but not for spores..
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