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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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OfflineBloodNOil
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Registered: 10/08/03
Posts: 1,020
Last seen: 15 years, 7 months
Substrate for an InVitro grow?
    #3900982 - 03/11/05 12:55 AM (19 years, 1 month ago)

I'm wanting to do an invitro project, as i don't quite have the space
for a complex (read: obsessively sterile) growing apparatus, but I'm
feeling a bit adventurous as far as substrates are concerned. Would
rye or wild bird seed be appropriate for fruiting invitro? Is there
another substrate that I'm overlooking? or should i just stick with BRF?


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It's like a koala bear crapped a rainbow in my brain!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Substrate for an InVitro grow? [Re: BloodNOil]
    #3901032 - 03/11/05 01:09 AM (19 years, 1 month ago)

Whole grains require a PC for sterilization and are not really suited for invitro cultivation. The PF tek works great for invitro.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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OfflineBloodNOil
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Re: Substrate for an InVitro grow? [Re: dog]
    #3901075 - 03/11/05 01:25 AM (19 years, 1 month ago)

Cool. Hopefully this will turn out better than my first attempt.

Anyways, off to buy jars, fish heater, and cooler from Wal-Mart at 2 AM.
NOBODY WILL SUSPECT A THING HAHA!

I'll try this with pint jars and liquid culture to satisfy my sense of adventure...


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It's like a koala bear crapped a rainbow in my brain!

Edited by BloodNOil (03/11/05 01:27 AM)

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OfflineBloodNOil
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Re: Substrate for an InVitro grow? [Re: BloodNOil]
    #3901152 - 03/11/05 02:51 AM (19 years, 1 month ago)

Al right. I've got a styrofoam cooler, a heat-bomb style heater, and 24 1/2 pint wide-mouth jars.

I think i scared the check-out lady at Wal-Mart though.
"Jars! I must have more jars!! The surface area for the clones must be optimized! OPTIMIZED!!"


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It's like a koala bear crapped a rainbow in my brain!

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OfflineXTCollection
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Registered: 02/23/05
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Last seen: 17 years, 3 months
Re: Substrate for an InVitro grow? [Re: BloodNOil]
    #3901345 - 03/11/05 06:12 AM (19 years, 1 month ago)

In AFOAF's experience, PF tek cakes shrink slightly when colonized, making in vitro an option. Grains dont usually shrink mach at all, until after the first flush.

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 3 months
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Re: Substrate for an InVitro grow? [Re: XTCollection]
    #3901865 - 03/11/05 10:02 AM (19 years, 1 month ago)

Yeah but your supposed to leave room. I've never had any problem with grain invitro.


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Substrate for an InVitro grow? [Re: scatmanrav]
    #3902061 - 03/11/05 10:37 AM (19 years, 1 month ago)

You'd be amazed at how well straight rye can perform invitro. Just hydrate the grains with coffee and expose to light from the day you inoculate. This is all straight rye, no vermiculite or compost, hydrated with 50% of drinking strength coffee.



RR

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 3 months
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Re: Substrate for an InVitro grow? [Re: RogerRabbit]
    #3902218 - 03/11/05 11:06 AM (19 years, 1 month ago)

Even with contams, youd be surprised.



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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

Extras: Filter Print Post Top
OfflineBloodNOil
Captain Zeep
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Registered: 10/08/03
Posts: 1,020
Last seen: 15 years, 7 months
Re: Substrate for an InVitro grow? [Re: scatmanrav]
    #3903389 - 03/11/05 02:22 PM (19 years, 1 month ago)

Hmmmmm.... Would coffee be an option with the PF tek? Is there a specific way to use the coffee?

Do I even need the coffee if i'm going to be innoculating with liquid culture?


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It's like a koala bear crapped a rainbow in my brain!

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OfflineIGnosticAbhorI
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Registered: 11/06/04
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Last seen: 10 months, 21 days
Re: Substrate for an InVitro grow? [Re: BloodNOil]
    #3903428 - 03/11/05 02:27 PM (19 years, 1 month ago)

Don't use coffee :thumbdown:
Just my .02

Gl

-Gnostic


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For the sake of those seeking knowledge.
I've provided some in my signature.
Double Tek Pictorial
Getting the most out of your casings!, A pinning strategy.
Soaking wbs - The Final Answer by Agar
50/50 Coco Coir versus 50/50 Peat Moss.

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OfflineBloodNOil
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Posts: 1,020
Last seen: 15 years, 7 months
Re: Substrate for an InVitro grow? [Re: IGnosticAbhorI]
    #3903462 - 03/11/05 02:32 PM (19 years, 1 month ago)

Those jars are beautiful. I'm going to build smaller and slightly
less-tasty versions of them.

Off to the hippie-mart to get some rye grain!


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It's like a koala bear crapped a rainbow in my brain!

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Offlinepelon
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Registered: 12/04/04
Posts: 31
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Last seen: 18 years, 3 months
Re: Substrate for an InVitro grow? [Re: dog]
    #3967212 - 03/24/05 11:22 PM (19 years, 27 days ago)

Whole rye grain works great for invitro. I use 1/2 gallon jars with 2-3 inches of cooked(and pc'd) grain on the bottom (only additive to grain is a pinch of gypsum). After full colonization I case it in the jar with 2-3 inches of a coco coir based 50/50+ mix. This picture is of the latest 1/2 gallon jar. well over 50 strong looking pins.


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