Liquid Substrate Question - Mushroom Cultivation

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Phake_ld
enthusiast

Registered: 10/08/01
Posts: 230
Last seen: 21 years, 5 months

Liquid Substrate Question

#486874 - 12/11/01 01:30 AM (22 years, 5 days ago)

If my turtle is using a liquid substrate to grow mycelium (Malt Extract with distilled water), would he be able to suck the water itself into a spore syringe to innoculate a jar and promote colinization? or does there have to be chunks of mycelium in the spore syringe to promote colinization?

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jokerGD
interestedindividual

Registered: 10/26/01
Posts: 284
Loc: Earth
Last seen: 15 years, 5 months

Re: Liquid Substrate Question

[Re: Phake_ld]
#487083 - 12/11/01 05:03 AM (22 years, 5 days ago)

I've done this tek just recently... with the malt.... my results were quite interestin....

Basically give it time, if the jar didnt get infected, it'll get really dark milky looking and shady.... Like, if the water looks clear after a few weeks, you're infected... eventually you'll see lil chunks of stuff swimming around... as long as it looks a lil fuzzy and you see sorta lil fuzzy stuff, and maybe a lil build up on the bottom, thats the good stuff....

check this out... this is a jar injected with a liquid mycelium solution (light malt extract) --->

---->

this one was innoculated 11/30 with a crap load of liquid mycelium... my friend made 6 pint jars, one got infected, the rest are all liquid mycelium

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DELUDE
CNS+PC

Registered: 11/02/01
Posts: 95
Loc: Bulgaria
Last seen: 18 years, 2 months

Re: Liquid Substrate Question

[Re: Phake_ld]
#487128 - 12/11/01 07:15 AM (22 years, 5 days ago)

Hay, I tried the liquid substrat just yesterday. I used honey though. Does any1 know how long it takes for the jars to get ready for inoculation?

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Stuck in a Body -=- Lost in a Mind!

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Hammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 20 years, 4 months

Re: Liquid Substrate Question

[Re: DELUDE]
#487145 - 12/11/01 07:46 AM (22 years, 5 days ago)

Not very long.. 3-5 days.. there should be mycelium floating in it by then.. remember to shake it up well daily.. generally shake the shit out of it before filling your sterile syringe for injections too.. this pretty much ensures you'll get some good hunks of mycelium up in there.

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Phake_ld
enthusiast

Registered: 10/08/01
Posts: 230
Last seen: 21 years, 5 months

Re: Liquid Substrate Question

[Re: Hammerloaf]
#487425 - 12/11/01 01:24 PM (22 years, 4 days ago)

so there has to be hunks of mycellium in the syringe inorder for the jars to colonize. Or would just using the water the mycelium is growing in be fine?

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sylo
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Registered: 10/29/00
Posts: 219
Last seen: 21 years, 7 months

Re: Liquid Substrate Question

[Re: Phake_ld]
#488357 - 12/12/01 05:12 AM (22 years, 4 days ago)

You don't need chunks of mycelium but a syringe needle could filter out what you do need. Another method is to take a piece of wire and make a small hook on the end. Heat the wire red hot and then fish a small string of mycelium from one jar and transfer it to another.

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MAIA
World-BridgerKartikeya (DftS)

Registered: 04/27/01
Posts: 7,391
Loc: Erra - 20 Tauri - M45 Sta...
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Re: Liquid Substrate Question

[Re: Phake_ld]
#488373 - 12/12/01 05:56 AM (22 years, 4 days ago)

It's advisible to knock the syringe near the needle junction (needle up) with your finger between innoculations to disrupt the mycellium acumulation in that place, otherwise the syringe can get stucked.

Peace,
MAIA

--------------------
Spiritual being, living a human experience ... The Shroomery Mandala

Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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