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Offlinemotatraehrehtom
Brick

Registered: 12/13/02
Posts: 157
Last seen: 21 years, 4 months
A few inoculation/substrate questions.
    #1165841 - 12/23/02 06:58 PM (21 years, 6 months ago)

Most of the time to inoculate my jars (72 pint jars), I just revenge one jar and put pea sized pellets of colonized substrate (up until now various flours) into the sterilized jars. Using this method, I usually lose 6-10 jars to contaminants. Can anyone suggest another method of inoculation (besides syringe or print), or a way to improve my current method. Also, I'm moving on to straight brown rice as a substrate, will my method of inoculation still work with my new substrate? Thanks.



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Did they get you to trade, your heroes for ghosts? Hot ashes for trees? Hot air for a cool breeze? Cold comfort for change? And did you exchange, a walk on part in the war, for a lead role in the cage?

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Anonymous

Re: A few inoculation/substrate questions. [Re: motatraehrehtom]
    #1165852 - 12/23/02 07:07 PM (21 years, 6 months ago)

Are you saying you dont want to use a syringe, or you dont want to inject spores? you could make a liquid mycelium syringe and it would tear through those rice jars :wink:
Only other way I can think of right now, besides syringe or print, is agar. You dont want to use agar if you are already loosing 10% to contams. I think a good liquid tek with some h2o2 might be just what you are looking for. Your gonna have to search for the teks though cause I dont have them marked :frown:

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Offlinemotatraehrehtom
Brick

Registered: 12/13/02
Posts: 157
Last seen: 21 years, 4 months
Re: A few inoculation/substrate questions. [Re: ]
    #1165855 - 12/23/02 07:07 PM (21 years, 6 months ago)

Can you tell me more about a liquid mycelium syringe? Thanks.


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Did they get you to trade, your heroes for ghosts? Hot ashes for trees? Hot air for a cool breeze? Cold comfort for change? And did you exchange, a walk on part in the war, for a lead role in the cage?

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Anonymous

Re: A few inoculation/substrate questions. [Re: motatraehrehtom]
    #1165870 - 12/23/02 07:28 PM (21 years, 6 months ago)

Here is one link I found

http://66.221.98.80/findorgrowthem.php?View=docs&doc=96

you might also try one of the honey water teks, just do a search for honey water.


Edited by AverageWhiteGuy (12/23/02 07:31 PM)

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Offlinemotatraehrehtom
Brick

Registered: 12/13/02
Posts: 157
Last seen: 21 years, 4 months
Re: A few inoculation/substrate questions. [Re: ]
    #1165872 - 12/23/02 07:30 PM (21 years, 6 months ago)

Thank you.


--------------------
Did they get you to trade, your heroes for ghosts? Hot ashes for trees? Hot air for a cool breeze? Cold comfort for change? And did you exchange, a walk on part in the war, for a lead role in the cage?

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Anonymous

Re: A few inoculation/substrate questions. [Re: motatraehrehtom]
    #1165873 - 12/23/02 07:32 PM (21 years, 6 months ago)

You are welcome!!

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