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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 19 years, 6 months
Liquid Substrate Question
    #486874 - 12/11/01 03:30 AM (20 years, 1 month ago)

If my turtle is using a liquid substrate to grow mycelium (Malt Extract with distilled water), would he be able to suck the water itself into a spore syringe to innoculate a jar and promote colinization? or does there have to be chunks of mycelium in the spore syringe to promote colinization?


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OfflinejokerGD
interestedindividual
Registered: 10/26/01
Posts: 284
Loc: Earth
Last seen: 13 years, 6 months
Re: Liquid Substrate Question [Re: Phake_ld]
    #487083 - 12/11/01 07:03 AM (20 years, 1 month ago)

I've done this tek just recently... with the malt.... my results were quite interestin....

Basically give it time, if the jar didnt get infected, it'll get really dark milky looking and shady.... Like, if the water looks clear after a few weeks, you're infected... eventually you'll see lil chunks of stuff swimming around... as long as it looks a lil fuzzy and you see sorta lil fuzzy stuff, and maybe a lil build up on the bottom, thats the good stuff....


check this out... this is a jar injected with a liquid mycelium solution (light malt extract) --->


---->




this one was innoculated 11/30 with a crap load of liquid mycelium... my friend made 6 pint jars, one got infected, the rest are all liquid mycelium




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OfflineDELUDE
CNS+PC

Registered: 11/02/01
Posts: 95
Loc: Bulgaria
Last seen: 16 years, 3 months
Re: Liquid Substrate Question [Re: Phake_ld]
    #487128 - 12/11/01 09:15 AM (20 years, 1 month ago)

Hay, I tried the liquid substrat just yesterday. I used honey though. Does any1 know how long it takes for the jars to get ready for inoculation?


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Stuck in a Body -=- Lost in a Mind!


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OfflineHammerloaf
addict

Registered: 11/26/01
Posts: 439
Loc: Canada
Last seen: 18 years, 5 months
Re: Liquid Substrate Question [Re: DELUDE]
    #487145 - 12/11/01 09:46 AM (20 years, 1 month ago)

Not very long.. 3-5 days.. there should be mycelium floating in it by then.. remember to shake it up well daily.. generally shake the shit out of it before filling your sterile syringe for injections too.. this pretty much ensures you'll get some good hunks of mycelium up in there.


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OfflinePhake_ld
enthusiast
Registered: 10/08/01
Posts: 230
Last seen: 19 years, 6 months
Re: Liquid Substrate Question [Re: Hammerloaf]
    #487425 - 12/11/01 03:24 PM (20 years, 1 month ago)

so there has to be hunks of mycellium in the syringe inorder for the jars to colonize. Or would just using the water the mycelium is growing in be fine?


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Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 19 years, 8 months
Re: Liquid Substrate Question [Re: Phake_ld]
    #488357 - 12/12/01 07:12 AM (20 years, 1 month ago)

You don't need chunks of mycelium but a syringe needle could filter out what you do need. Another method is to take a piece of wire and make a small hook on the end. Heat the wire red hot and then fish a small string of mycelium from one jar and transfer it to another.


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OfflineMAIA
World-BridgerKartikeya (DftS)
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Registered: 04/27/01
Posts: 7,351
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Re: Liquid Substrate Question [Re: Phake_ld]
    #488373 - 12/12/01 07:56 AM (20 years, 1 month ago)

It's advisible to knock the syringe near the needle junction (needle up) with your finger between innoculations to disrupt the mycellium acumulation in that place, otherwise the syringe can get stucked.

Peace,
MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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