Home | Community | Message Board

Cannabis Seeds UK
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinefinite
journeyman
Registered: 08/28/00
Posts: 2
Last seen: 20 years, 11 months
will a strain repeatedly cloned lose its vigor?
    #329205 - 05/30/01 05:52 AM (21 years, 4 months ago)

A question about the process of cloning an isolated strain with mycelia...
I have found a variant amoung a group of 14 jars which were all inocculated with the same
Gulf Coast print from Homestead. The mycelia in this one jar produces mushrooms totally
unlike what the others produced. They are fat, tall, potent, and twisted looking. I managed
to inocculate more jars with mycelia from this strain (it wasn't really a "sterile" technique, but it seemed to work) and plan to case some of them but use some of them as "clone jars" so I can continue
to keep this strain growing. I know taking a spore print will not mean the resulting mycelia
will in fact be identical to the "parent" mushroom. Cloning with mycelia seems to work, I was
just wondering if the strain will lose its vigor if it is used continuously. I know you can clone
with tissue, but why would that be any different than with a pure strain of mycelia?



Extras: Filter Print Post Top
Invisibledimitri211
Carpal Tunnel
Registered: 04/28/01
Posts: 2,248
Re: will a strain repeatedly cloned lose its vigor? [Re: finite]
    #329252 - 05/30/01 06:54 AM (21 years, 4 months ago)

Yes it can lose it's vigor P. Stamets uses the p. value system mean the he only transfer mycelium 10 time from agar to agar--If you use a different substrate each time you will do o.k. finch seed then rye grain then wheat grain etc...

www.thehawkseye.com
Sacred Mushroom Spores
----------------------------------------------
Donations to the shroomery
www.paypal.com (Account thor@shroomery.org)


Extras: Filter Print Post Top
Invisiblellamabox
Myco/Ethnoresearcher
Male User Gallery

Registered: 11/24/05
Posts: 564
Loc: Third moon of the Indole ...
Re: will a strain repeatedly cloned lose its vigor? [Re: dimitri211]
    #5003252 - 12/02/05 01:52 AM (16 years, 9 months ago)

Would there be any way to breed a specific clone back to its parent mycelium to stabilize the strain?


--------------------

Free Ethnobotanical Seed Ring


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,213
Loc: Seattle
Last seen: 15 days, 18 hours
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: llamabox]
    #5006096 - 12/02/05 06:45 PM (16 years, 9 months ago)

Just keep those 'clone jars' in the refrigerator. Only take them out when you need to do a grain to grain transfer. They should last for a year or two that way, then simply do a g2g and keep one of those jars back as a strain master.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: llamabox]
    #5012981 - 12/04/05 02:28 PM (16 years, 9 months ago)



You can store G-1 LC in 5, 50 or 100 vials of sterile water (years & years).

Any time you want G-1 pull a tube out of the Fridge & go for it.

Either start a new LC with it, or go to grains, then G2G.


--------------------


Extras: Filter Print Post Top
Offlinemattymonkey
Feel Like aStranger...
 User Gallery

Registered: 11/08/04
Posts: 973
Last seen: 9 years, 8 months
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5013063 - 12/04/05 04:45 PM (16 years, 9 months ago)

agar- just put a few cc of LC into a vial of sterile water?


--------------------
"listening for the secret.. searching for the sound.."


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: mattymonkey]
    #5015776 - 12/05/05 07:04 AM (16 years, 9 months ago)

Quote:

mattymonkey said:
agar- just put a few cc of LC into a vial of sterile water?




You put the LC solution in a rubber stoppered tube.

Allow the myc to settle to the bottom.


You then aspirate out the solution, leaving the myc in the bottom.

(that removes most nutrients).

You then inject sterile water into the tube.


Then refrigerate that tube.

It will remain viable for years.


--------------------


Extras: Filter Print Post Top
Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Qu├ębec, Canada
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5016563 - 12/05/05 12:20 PM (16 years, 9 months ago)

Agar- How do you aspirate out the solution without risk of infections?


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: micololo2]
    #5016659 - 12/05/05 03:16 PM (16 years, 9 months ago)

Soak a few cottom balls in alcohol. Swab down the surface of what you asperate out of, or inject into. Keep the needle wrapped in that alc soaked cotton ball, so it is not exposed to open air.


--------------------


Extras: Filter Print Post Top
OfflineBlue Helix
bold hand
Male User Gallery

Registered: 02/02/03
Posts: 1,565
Last seen: 1 month, 19 days
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5021444 - 12/06/05 09:55 AM (16 years, 9 months ago)

I would recommend asperating through the rubber cap. Just inject the air and suck in succession until the free liquid is out. Removing the rubber cap is asking for trouble unless you have a glove box or flow hood.


Extras: Filter Print Post Top
Invisibleagar
old hand
 User Gallery

Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: Blue Helix]
    #5021713 - 12/06/05 11:08 AM (16 years, 9 months ago)

>>>Removing the rubber cap is asking for trouble unless you have a glove box or flow hood.<<<

True that.


--------------------


Extras: Filter Print Post Top
Offlinerungi
journeymana

Registered: 07/12/01
Posts: 212
Last seen: 13 years, 8 months
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #6246408 - 11/04/06 06:19 PM (15 years, 10 months ago)

Buffer the medium at various pH values. This causes many mycelia morphologies to differentiate. A clone from a single pure plate to plates of differing pH will all exhibit different growth morphologies.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,213
Loc: Seattle
Last seen: 15 days, 18 hours
Trusted Cultivator
Re: will a strain repeatedly cloned lose its vigor? [Re: finite]
    #6248655 - 11/05/06 08:05 AM (15 years, 10 months ago)

This thread has been closed.

Reason:
Let's let this old thread on senescence die and go to heaven.
RR


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Bulk Substrate, Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Bridgetown Botanicals CBD Concentrates   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Autoflowering Cannabis Seeds, Bulk Cannabis Seeds, Feminized Cannabis Seeds, High THC Strains, USA West Coast Strains   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale


Similar ThreadsPosterViewsRepliesLast post
* Re: Preserving my strains LillSkit 1,759 6 02/17/00 08:27 AM
by Anonymous
* 2 different strains in one jar? I_Fart_Blue 2,639 6 07/10/02 09:49 PM
by jdtwelve
* Crossing two strains IS possible..Heres how...
( 1 2 3 4 5 all )
BlueJay 33,103 96 06/02/15 07:34 AM
by God25
* clone tissue... the picking!!! PooPs 4,143 10 06/16/03 08:34 PM
by PooPs
* Re: Mushroom Strain Improvement and Breeding? Ripper 4,710 10 02/10/00 04:01 PM
by Anonymous
* Does mycelia lose vigor or viability? On_the_Down-Low 899 3 02/16/03 05:23 PM
by On_the_Down-Low
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 8,638 18 07/24/01 09:02 AM
by Anno
* Getting a strain isolate without agar ??
( 1 2 3 4 all )
majik_monkey 13,626 61 01/14/04 07:44 AM
by Joshua

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte, bodhisatta
3,463 topic views. 1 members, 13 guests and 1 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2022 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 13 queries.