Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
will a strain repeatedly cloned lose its vigor?
    #329205 -

A question about the process of cloning an isolated strain with mycelia...
I have found a variant amoung a group of 14 jars which were all inocculated with the same
Gulf Coast print from Homestead. The mycelia in this one jar produces mushrooms totally
unlike what the others produced. They are fat, tall, potent, and twisted looking. I managed
to inocculate more jars with mycelia from this strain (it wasn't really a "sterile" technique, but it seemed to work) and plan to case some of them but use some of them as "clone jars" so I can continue
to keep this strain growing. I know taking a spore print will not mean the resulting mycelia
will in fact be identical to the "parent" mushroom. Cloning with mycelia seems to work, I was
just wondering if the strain will lose its vigor if it is used continuously. I know you can clone
with tissue, but why would that be any different than with a pure strain of mycelia?


Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: finite]
    #329252 -

Yes it can lose it's vigor P. Stamets uses the p. value system mean the he only transfer mycelium 10 time from agar to agar--If you use a different substrate each time you will do o.k. finch seed then rye grain then wheat grain etc...

www.thehawkseye.com
Sacred Mushroom Spores
----------------------------------------------
Donations to the shroomery
www.paypal.com (Account thor@shroomery.org)


--------------------
Dr.Bluethumbs
RAFFLE to help out GS and his 7 month old daughter

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: dimitri211]
    #5003252 -

Would there be any way to breed a specific clone back to its parent mycelium to stabilize the strain?


--------------------

Free Ethnobotanical Seed Ring

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: llamabox]
    #5006096 -

Just keep those 'clone jars' in the refrigerator. Only take them out when you need to do a grain to grain transfer. They should last for a year or two that way, then simply do a g2g and keep one of those jars back as a strain master.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: llamabox]
    #5012981 -



You can store G-1 LC in 5, 50 or 100 vials of sterile water (years & years).

Any time you want G-1 pull a tube out of the Fridge & go for it.

Either start a new LC with it, or go to grains, then G2G.


--------------------

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5013063 -

agar- just put a few cc of LC into a vial of sterile water?


--------------------
"listening for the secret.. searching for the sound.."

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: mattymonkey]
    #5015776 -

mattymonkey said:
agar- just put a few cc of LC into a vial of sterile water?


You put the LC solution in a rubber stoppered tube.

Allow the myc to settle to the bottom.


You then aspirate out the solution, leaving the myc in the bottom.

(that removes most nutrients).

You then inject sterile water into the tube.


Then refrigerate that tube.

It will remain viable for years.


--------------------

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5016563 -

Agar- How do you aspirate out the solution without risk of infections?

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: micololo2]
    #5016659 -

Soak a few cottom balls in alcohol. Swab down the surface of what you asperate out of, or inject into. Keep the needle wrapped in that alc soaked cotton ball, so it is not exposed to open air.


--------------------

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #5021444 -

I would recommend asperating through the rubber cap. Just inject the air and suck in succession until the free liquid is out. Removing the rubber cap is asking for trouble unless you have a glove box or flow hood.

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: Blue Helix]
    #5021713 -

>>>Removing the rubber cap is asking for trouble unless you have a glove box or flow hood.<<<

True that.


--------------------

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: agar]
    #6246408 -

Buffer the medium at various pH values. This causes many mycelia morphologies to differentiate. A clone from a single pure plate to plates of differing pH will all exhibit different growth morphologies.

Extras: Filter Print Post Top
Re: will a strain repeatedly cloned lose its vigor? [Re: finite]
    #6248655 -

This thread has been closed.

Reason:
Let's let this old thread on senescence die and go to heaven.
RR

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Mushroom-Hut Substrate Bags   Myyco.com Golden Teacher Liquid Culture For Sale   Bridgetown Botanicals CBD Concentrates   Original Sensible Seeds Autoflowering Cannabis Seeds   MagicBag.co All-In-One Bags That Don't Suck

Mushrooms, Mycology and Psychedelics > Advanced Mycology
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Re: Preserving my strains LillSkit 1,892 6 02/16/00 11:27 PM
by Anonymous
* 2 different strains in one jar? I_Fart_Blue 2,936 6 07/10/02 12:49 PM
by jdtwelve
* Crossing two strains IS possible..Heres how...
( 1 2 3 4 5 all )		 BlueJay 37,534 96 06/01/15 10:34 PM
by God25
* clone tissue... the picking!!! PooPs 4,286 10 06/16/03 11:34 AM
by PooPs
* Re: Mushroom Strain Improvement and Breeding? Ripper 5,212 10 02/10/00 07:01 AM
by Anonymous
* Does mycelia lose vigor or viability? On_the_Down-Low 953 3 02/16/03 08:23 AM
by On_the_Down-Low
* Single Strain (spore) Isolation and the Benefits.. TrippinRhino 9,364 18 07/24/01 12:02 AM
by Anno
* Getting a strain isolate without agar ??
( 1 2 3 4 all )		 majik_monkey 14,464 61 01/13/04 10:44 PM
by Joshua

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta
3,655 topic views. 0 members, 1 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.011 seconds on 14 queries.