CeeGood said:

As posted before, I have two different strains in each bag.

GT that I used a 10ml spore syringe on and this is day 7 and I think it’s coming along nicely.



Second bag was PE that I think was a mycelium liquid culture and grew fast but thin on the plate. Used only 5ml on the bag and saw some white starting at the bottom on day 2 or 3, but never progressed anymore. I’m thinking because I only used 5ml?

Should I add more to the bag at this point or should I add some sterile water because it’s very dry in there.

---

No need to add more innoculant or water that millet looks hydrated to me. 5ml should do it, 10ml would be faster, however by using less you simply need to wait longer for colonization. You need to save some of that PE LC so you can verify on another plate or two. It is a moldy plate I saw in the last post. Either there's a hole in your sterile technique, or the LC is contaminated. Best to figure that out first as try and preserve the genetics at hand. Best of luck

CloudNyne420 said:

So i have sum agar plates here n i was wondering if these looked gud to u guys or not?? I feel like there gud i jus wana make sure with u all as best i can anyway.

Plate 1



Plate 2

---

krinkle said:

Hi,

I previously posted 3 days ago about potential mycogone.

I've taken updated photo's today, finally felt confident enough to remove the lid entirely to get better photo's of the potential spots. Some of the spots that previously looked like mycogone appear to no longer have the metabolite coloured droplets on them and the mycelium has "deflated" for lack of a better term. It's not as fluffy as it used to be is what I guess I'm trying to say.

Got an overall shot of the cake as well. I tried my best to keep the lid parallel to the base of the tub as I lifted but unfortunately a bunch of droplets still fell onto the mycelium. I ended up mopping up the remainder of the droplets from the lid, not sure if that was a good idea. RH has been between 45-55% in the room and I still have micropore tape covering the holes  of the tub.

I did find that all the potential mycogone spots only appear around the edge of the cake, close to where it meets the walls of the tub, not sure if the means anything. I'm hoping it was just metabolites as this is my first success at getting this far.

Hypothetically if any spots were to develop into mycogone is there any potential methods to save the cake or would any fruits no longer be safe for consumption?

---

Bigworm said:

I hope so cause mine looked really similar to that and I sent it a couple days ago.

---

Cakewonder said:

DO MORE PF TEK!!!!

---

hunter465 said:

When dealing with bags how often do people do break and shakes or just shakes?

I was a little impatient after I inoculated. Waited about 3 days till I swirled around the bags till I found some mycelium clumps, broke those up and shook the bags.

Have been shaking around the bags everyday since and it seems the bags are about 30% colonized at this point, a week in from inoculation.

Anybody else done this?

Is there a chance this will stall things in the long run?

What is the fastest time to 100% colonization of a bag people have seen?

---