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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29721540 -

DV8DUG said:
Fiet said:
Guy's i hope you see this you ask for open lid Pics...


Unfortunately both of those look bad. See how the outside edge is disorganized like someone dropped a bunch of white sticks? The mycelium you're looking for has intelligent, organized growth. The first plate has a giant halo going on.

The plate I thought had hope was the fourth pic in the first set of pix. The one with the large droplets of condensation and the big triangle shaped transfer.

Check out the first link in my sig.






Hey,

my Grain Jars looking good i think it's Jack Frost. I make a LC from Agar dishes but from dishes some weeks ago...

I still have a LC from the Jack and DC would you Drop it on Agar ore not Worth it?

It's shitty sad my Dishes looks not clean so i can throw them away? Good i have a LC from the Grain Jars the LC is Clean i think ore?

I do have a Dish in a fridge Agar it looks good but i transfer and the Dishes looks what i showing you.

Hmmm

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet] * 1
    #29721591 -

Planning on harvesting my grow tomorrow. I'm wondering if the few newly torn caps will sporulate by then and make a mess? The gills seem super light still. Should I harvest tonight instead?





:tongue2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Mystikal]
    #29721594 -

I forget, should syringes be horizontal or vertical during the pc cycle to prevent the water from boiling out? If vertical, I assume plunger end up? Idk why I started having issues with syringes drying up, I must be doing something differently than I used to.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: Turvenuija]
    #29721605 -

Needle up. I've read it somewhere can't remember where but I did it the other day.:muppet:


--------------------
Some say we'll see Armageddon soon
I certainly hope we will
:madpirate:

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Re: Ask Quick Questions, Get Quick Answers [Re: Reishi Uchiha]
    #29721611 -

Where is best place to take transfer when doing cabin do i need to transfer from agar that i use for cabin roof or better to wait to spread around the plate?

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Re: Ask Quick Questions, Get Quick Answers [Re: maimunji] * 1
    #29721619 -

Yup, needle up. When water becomes steam, its volume expands by over 1500x.

All those bubbles of steam will push out water. Any option where the bubbles can't go upwards and exit via thd needle, almost guarantees that all water is pushed out. See it as a small percolator.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Ask Quick Questions, Get Quick Answers [Re: Fiet] * 1
    #29721622 -

Fiet said:
DV8DUG said:
Fiet said:
Guy's i hope you see this you ask for open lid Pics...


Unfortunately both of those look bad. See how the outside edge is disorganized like someone dropped a bunch of white sticks? The mycelium you're looking for has intelligent, organized growth. The first plate has a giant halo going on.

The plate I thought had hope was the fourth pic in the first set of pix. The one with the large droplets of condensation and the big triangle shaped transfer.

Check out the first link in my sig.






Hey,

my Grain Jars looking good i think it's Jack Frost. I make a LC from Agar dishes but from dishes some weeks ago...

I still have a LC from the Jack and DC would you Drop it on Agar ore not Worth it?

It's shitty sad my Dishes looks not clean so i can throw them away? Good i have a LC from the Grain Jars the LC is Clean i think ore?

I do have a Dish in a fridge Agar it looks good but i transfer and the Dishes looks what i showing you.

Hmmm




--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: Churpee Choozle]
    #29721623 -

Churpee Choozle said:
Yup, needle up. When water becomes steam, its volume expands by over 1500x.

All those bubbles of steam will push out water. Any option where the bubbles can't go upwards and exit via thd needle, almost guarantees that all water is pushed out. See it as a small percolator.


Oh yeah, that makes sense. Thanks.


--------------------

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Re: Ask Quick Questions, Get Quick Answers [Re: maimunji] * 2
    #29721627 -

maimunji said:
Where is best place to take transfer when doing cabin do i need to transfer from agar that i use for cabin roof or better to wait to spread around the plate?


Always transfer the best looking of what you got. Edges.

Follow the first link in my sig.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: maimunji] * 2
    #29721635 -

maimunji said:
Where is best place to take transfer when doing cabin do i need to transfer from agar that i use for cabin roof or better to wait to spread around the plate?


The best is probably from the roof itself, but then you would need to take an aerial mycelium transfer which can be a little tricky. If you don't want to do that just let it grow out some and as DV8 said, pick the best spot(s).


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 1
    #29721639 -

Already read it many times thank you.

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29721641 -

Thanks Kloakk. I experiment a bit taking air mycelium i think drop it to new plate is a hard part of it...its just dont want to goes of from scalpel. I understand will need practice.

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Re: Ask Quick Questions, Get Quick Answers [Re: maimunji] * 4
    #29721655 -

maimunji said:
Thanks Kloakk. I experiment a bit taking air mycelium i think drop it to new plate is a hard part of it...its just dont want to goes of from scalpel. I understand will need practice.


You and me both man, I recently read about how Fahtster takes aerial transfers to clean up pan plates, it made a lot of sense to me, with all transfers that isn't meant to be used for inoculation and I have been practicing myself for the last couple of sessions.

I've tried several tools, but I am back to using my scalpel with a #11 blade. The highest success so far is to hold my plate almost vertically and use the dull side of the blade to scrape a small piece of mycelium on, I then turn my scalpel with the sharp side down and slightly cut into the new plate and pull it back so that the mycelium will get contact with the agar.

I'll see if I can make a gif or something, but the transfer is so small I'm not sure how helpful it would be :lol:


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29721672 -

That's good advice. I saw a video of a guy using clear tape to get Ariel hyphae and then tape it down to a slide for a microscope.


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG]
    #29721690 -

QQ: how long can I keep a casing (peat moss, vermiculite and calcium carbonate) in a jar after pasteurisation before using it? And if that time was exceeded, can I just pasteurise it again? Or is it better to start over?

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Re: Ask Quick Questions, Get Quick Answers [Re: DV8DUG] * 8
    #29721695 -

DV8DUG said:
That's good advice. I saw a video of a guy using clear tape to get Ariel hyphae and then tape it down to a slide for a microscope.


Well, DV8 approved, let's do this thing then :rockon:



ambigu said:
QQ: how long can I keep a casing (peat moss, vermiculite and calcium carbonate) in a jar after pasteurisation before using it? And if that time was exceeded, can I just pasteurise it again? Or is it better to start over?


As long as you have good gas exchange I have used a casing that was 10 days old without re-pasteurizing it, I have re-pasteurized a 3 week old casing with success. Use your nose, anything smells off yeet it into the sun.


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


Edited by Kloakk (11/07/26 05:17 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 1
    #29721707 -

Thanks. It doesn’t smell sour or any different from when it was pasteurised, and I’ve left the lids slightly open for gas exchange. I think it might have been 4 weeks already that I pasteurised it, and I’ll need it in about a week, so I’ll make sure to repasteurise.

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Re: Ask Quick Questions, Get Quick Answers [Re: ambigu] * 1
    #29721712 -

ambigu said:
Thanks. It doesn’t smell sour or any different from when it was pasteurised, and I’ve left the lids slightly open for gas exchange. I think it might have been 4 weeks already that I pasteurised it, and I’ll need it in about a week, so I’ll make sure to repasteurise.


It might be fine, I mean I've had the same casing sitting 4 months on one of my Papuana trays before they decided to show up, but yeah I would re-pasteurize it a day or two before I was using it.


--------------------
β€œAll Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29721769 -

No gloves Kloakk? Hardcore...:takingnotes:


--------------------
Agar Plates and Removing Hidden Contaminants - A Visual Notebook
Recognizing Contamination in Grain Jars - A Visual Notebook
Contaminated Condiment Cup Cleanup v1.0

Green Cap Pheno Isolation
πŸ…ƒπŸ„΄πŸ„°πŸ„Ό ​ πŸ„²πŸ„ΎπŸ„½πŸ„³πŸ„ΈπŸ„ΌπŸ„΄πŸ„½πŸ…ƒ ​ πŸ„²πŸ…„πŸ„Ώ

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Re: Ask Quick Questions, Get Quick Answers [Re: ambigu] * 3
    #29721770 -

ambigu said:
QQ: how long can I keep a casing (peat moss, vermiculite and calcium carbonate) in a jar after pasteurisation before using it? And if that time was exceeded, can I just pasteurise it again? Or is it better to start over?


Freeze it bruh


--------------------
"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs

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Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   North Spore Cultivation Supplies

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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