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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Official Copelandia/Panaeolus Thread [Re: ghiajake] * 6
    #29706211 -

ghiajake said:
This last test bag smelled perfect last night when I spawned it.



Awesome!! Heres looking forward to success!!

Here’s a couple of pictures of teeny, tiny chlorocystis. First flush from a super clean germ plate. T1 trays a currently colonising. Red milo for grain, Faht’s sub recipe with a little blitzed straw added because i want to use it up. Enough fruit here for me and my partner to enjoy Saturday watching cartoons.





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Re: Official Copelandia/Panaeolus Thread [Re: tomtacy] * 3
    #29706214 -

Don't spray them, most pans do not like that. After harvest, you can rehydrate the casing or, when the casing looks like crap, remove it and re-case. You can spread out the harvest, ie let the pins go for a bit longer. I normally just remove everything and then soak the crap out of it.


--------------------
No nation, no border
Fight law and order


Species grown so far

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle] * 3
    #29706216 -

I like your species resume, Churp!


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: MomoMyco] * 2
    #29706219 -

MomoMyco said:
ghiajake said:
This last test bag smelled perfect last night when I spawned it.



Awesome!! Heres looking forward to success!!

Here’s a couple of pictures of teeny, tiny chlorocystis. First flush from a super clean germ plate. T1 trays a currently colonising. Red milo for grain, Faht’s sub recipe with a little blitzed straw added because i want to use it up. Enough fruit here for me and my partner to enjoy Saturday watching cartoons.







Nice.  I was never able to get chloros to fruit after a few tries.  I didn’t try a live casing tho.  I think that became a thing for them after I had given up on em.

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Re: Official Copelandia/Panaeolus Thread [Re: Churpee Choozle]
    #29706226 -

ghiajake said:
Generally it's best to harvest everything at once. IMO anyways.


Churpee Choozle said:
Don't spray them, most pans do not like that. After harvest, you can rehydrate the casing or, when the casing looks like crap, remove it and re-case. You can spread out the harvest, ie let the pins go for a bit longer. I normally just remove everything and then soak the crap out of it.


Thanks for you input, I will harvest everything that grew over the ankles and syringe all the casing to give pinheads the second chance.

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 1
    #29706841 -

Thanks man. Both my Chloro runs have been successful but I put it down to beautifully clean swabs. They were just pristine.

I’ve found live casing helps with all the homothalics and any pan that struggles to fruit. I’ve recased and added grass seed to trays that have overIayed to get successful grows before. I know there’s been talk about symbiosis, bacterial support etc but I’m leaning towards just being a huge help in maintaining the correct surface conditions.

Edited by MomoMyco (10/24/26 03:11 AM)

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Re: Official Copelandia/Panaeolus Thread [Re: tomtacy] * 3
    #29706845 -

I obliterate everything at harvest, all the pins get taken out as well. I scrape all the detritus off under running water which also helps to rehydrate. Then I’ll recase the tray before fruiting again. I think it was Tree Frog who described their process and I copied.

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Re: Official Copelandia/Panaeolus Thread [Re: MomoMyco] * 1
    #29707512 -

Okay,

@ Baba
@ Faht
@ Puff
@ Kloakk

Here is what I got with those clones these pictures are specifically

Clone sample > Water Agar
Water Agar > Low Nute Agar

,, so far no cabin,, yet. No trench or trap door. Based on Faht's pictures from when i first took the clones Here I think these are doing pretty good. Faht said to watch out for non-areal myc and so far, all of these plates have areal myc and no thick overlay. Still a little young on the transfer going to let it grow more but thoughts and opinions??



Clone 1




Clone 2




Clone 3




Clone 4




Clone 5




Clone 6



--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 2
    #29707527 -

Lo-Z said:
Okay,

@ Baba
@ Faht
@ Puff
@ Kloakk

Here is what I got with those clones these pictures are specifically

Clone sample > Water Agar
Water Agar > Low Nute Agar

,, so far no cabin,, yet. No trench or trap door. Based on Faht's pictures from when i first took the clones Here I think these are doing pretty good. Faht said to watch out for non-areal myc and so far, all of these plates have areal myc and no thick overlay. Still a little young on the transfer going to let it grow more but thoughts and opinions??



Clone 1




Clone 2




Clone 3




Clone 4




Clone 5




Clone 6






--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Official Copelandia/Panaeolus Thread [Re: Kloakk] * 1
    #29707536 -

Kloakk said:




Don't play with my emotions like that Kloakk,,,
:imdying:

you think it's looking good? Mind you this was from that bacterial tub. So far to me it looks good, but if you think it looks good i wont question it.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 2
    #29707538 -

I've had post-CS plates look not as nice as those.

+1 for send it and pick the nicest ones and put a small piece through a CS.

Go get those mushrooms


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Copelandia/Panaeolus Thread [Re: PuffM4gicDr4gon]
    #29707543 -

PuffM4gicDr4gon said:
I've had post-CS plates look not as nice as those.

+1 for send it and pick the nicest ones and put a small piece through a CS.

Go get those mushrooms



so these were my WA to Low nute transfers.

I have a few transfers from the water agar straight into cabins, i did agar wedge's island tek too. 2 trenches and a trap door. but those are understandably lot slower than straight transfers.

That's a 2 for 2 on sending it. I Think I'll do that ! including your advice to send 2 of the best through cabins.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 3
    #29707567 -

They are looking really good. Take a transfer and send the rest.


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 5
    #29707573 -

They look good to me.  I’d still do aerial myc transfers and keep that going.  At best, it’ll clean the cultures more each time you do one and at worst, it’ll maintain the cleanliness you have now.  If you do agar wedge transfers and there’s bacteria on the agar wedge, it’ll just compound the problem with each transfer until it’s eventually an unusable culture, IME anyway. 

It’s a numbers game.. if there’s biofilm on the agar wedge, it’s an established colony and very quickly will migrate to the new transfer plate.  Aerial myc transfers may have free floating bacteria on it but the amount of mycelium out numbers those free floating Bacteria.  The myc will establish itself on the new transfer plate first (depending on the aggressiveness of the bacteria) and outrun the bacteria.  If you keep doing this multiple times, the aerial myc should have less and less free floating bacteria on it as it gets further and further away from newly established bacterial colonies on the new plates

Not saying don’t send those plates, just that you should take an aerial transfer from the leading edge before you send them to keep cleaning the culture up

It’s all about numbers with pan.. gotta do a shit load of clones and then a shit load of transfers.  Eventually, you’ll get to a point where you either say, “this looks really good” and send it or you’ll say, “I’ve done 5 aerial transfers and this is as good as this culture is going to get” and you send it.  There’s only one way to test a culture and that’s to grow it out.  Ideally, you’ll want to keep track of each culture and what happens when you send it.  That way, you can either confirm it’s cleanliness or know there’s more work to do.

Keeping your old early transfer plates around is a good idea too.. a fully grown Out plate will tell you more about the state that plate is actually in as far as how clean it was.  You want to watch out for leading edges that crash out or discoloration under the myc.  Early on, a plate may not show discoloration but as the plate grows out, the biofilm builds and that discoloration is more pronounced.  So keep your old plates around for a while and check on them as they grow out.  It all helps inform how you should move forward with the culture.

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Re: Official Copelandia/Panaeolus Thread [Re: fahtster] * 2
    #29707752 -

https://www.shroomery.org/forums/showflat.php?Cat=0&Number=29701994&page=0&vc=1#29701994

Journal I've started up on a early Master Pan jars and some bulk pipelines

Still a WIP but hope to journal all the way to fruiting (versus straight grain)

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Re: Official Copelandia/Panaeolus Thread [Re: Snazz] * 2
    #29707819 -

Thank you guys for all the support and information on this journey of mine. I would have been failing long and hard had it not been for you guys.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
Re: Official Copelandia/Panaeolus Thread [Re: fahtster]
    #29707923 -

Hello! Do you pay attention to temperatures of the place where you store your pan cultures?

At what temperatures is the growth hindered?

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Re: Official Copelandia/Panaeolus Thread [Re: Lo-Z] * 1
    #29707935 -

Are you just asking for culture growth on plates and such or is this in regards to fruiting as well?


--------------------
🍄PANAEOLUS IN MONOTUBS🍄
🍄OFFICIAL SEMILANCEATA THREAD🍄



⭐Baba's Write Ups and Link Collection⭐

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Re: Official Copelandia/Panaeolus Thread [Re: Baba Yaga]
    #29707946 -

Just about plate growth

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Re: Official Copelandia/Panaeolus Thread [Re: Muffa] * 1
    #29707997 -

The pictures of plates above that I asked Baba, Faht, Kloakk, and Puff about were stored in 74-76°f.


--------------------
A Seeker Seeking,,, Things...

Lo-Z Things
State of The Grow :awesomenod: | Lo-Z's Forever Print Tek :print:
Brown Rice Grain Prep (Step by Step) :syringe: | My Favorite Cow PooPoo Sub Tek :poop:

Things that saved Lo-Z
PANS IN TUBS :mushroom2:
Rescuing Your AIO Kit :lifesaver:

Extras: Filter Print Post Top
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Shop: North Spore Injection Grain Bag   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Autoflowering Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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