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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 3
    #29600998 -

Magnesium will make the gel more elastic but I prefer Calcium Chloride which creates a brittle network. That's how I'm able to easily create a liquid inoculant with a stir bar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: strawboy]
    #29601008 -

strawboy said:
Magnesium will make the gel more elastic but I prefer Calcium Chloride which creates a brittle network. That's how I'm able to easily create a liquid inoculant with a stir bar.


Mind sharing the recipe? Also, do you use a different recipe for plates for cabins or plates  that's not going to be used for LI?

:takingnotes:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 2
    #29601017 -

You can go with .2g or .3g of Calcium Chloride for cabins. 0.1 for slurry. 0.2g for an all arounder.
https://www.shroomery.org/forums/showflat.php/Number/29508830

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Re: AGAR ENVY! (Anything and All things agar!) [Re: strawboy]
    #29601027 -

strawboy said:
You can go with .2g or .3g of Calcium Chloride for cabins. 0.1 for slurry. 0.2g for an all arounder.
https://www.shroomery.org/forums/showflat.php/Number/29508830


:nice:

Thank you :hatsoff:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 2
    #29601085 -

You can't just replace the chloride with your lactate though. Lactate is heavier

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Re: AGAR ENVY! (Anything and All things agar!) [Re: strawboy]
    #29601569 -

strawboy said:
You can't just replace the chloride with your lactate though. Lactate is heavier


Yeah, so the elemental calcium concentration for chloride is 27% and 13% for lactate. Would it make sense to double the calcium amount if I'm using lactate?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 2
    #29601662 -

My Calcium Chloride is anhydrous so it's 36% elemental calcium. You can multiply my measurements by 2.8

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Re: AGAR ENVY! (Anything and All things agar!) [Re: strawboy]
    #29601753 -

strawboy said:
My Calcium Chloride is anhydrous so it's 36% elemental calcium. You can multiply my measurements by 2.8


:highfive:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29602380 -

General question for anyone- When you use grain water to make plates how much grain water do you use for 500ml water & 10g agar agar? I usually use like 7.5g LME but wanted to try grain water if I am already producing it and there isn’t a difference.


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*Not a therapist - Do not take advice from me

Be nice. You are loved. You deserve happiness. You are not alone.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: JoeTherapist]
    #29602656 -

Looks like it’s being nibbled on the edges? It was fairly uniform a couple days ago.


MITE be a problem:confused:


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It’s not personal

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Re: AGAR ENVY! (Anything and All things agar!) [Re: idontknow1]
    #29603036 -



I know you all say blue is cool, but, um, yeah. Tmac on PDA, think Ill put some of the original wedge onto an antibiotic plate and see how it goes.


--------------------

I'm here for the medicine and the awakening

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mindfuel] * 2
    #29603039 -

mindfuel said:


I know you all say blue is cool, but, um, yeah. Tmac on PDA, think Ill put some of the original wedge onto an antibiotic plate and see how it goes.




--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 1
    #29603049 -

Classic! :rockon:


--------------------

I'm here for the medicine and the awakening

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk] * 2
    #29603054 -

Kloakk said:
mindfuel said:


I know you all say blue is cool, but, um, yeah. Tmac on PDA, think Ill put some of the original wedge onto an antibiotic plate and see how it goes.





Accurate.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Boozie] * 1
    #29603227 -

Some Mexicana hybrid called "Planet 9" (ATL#7 x Mystery #2)
Showing signs of "tanning" on agar and also has some huge bumps on the germination plate which I think are stones forming? not sure but definitely keeping my eye on these plates.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fantastic Mr. Fox] * 1
    #29604049 -

Is this dot mold on my x7x+ or did I drop a piece of myc last time when transferring? I transferred away from it just in case. Otherwise these look pretty clean to me but how do they look to you?




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Re: AGAR ENVY! (Anything and All things agar!) [Re: Turvenuija] * 1
    #29604050 -

Hard to tell from the photo if it's mold or bacteria, but it's a colony of something contaminating that plate. When I see a satellite like that appear so far away from the inoculant, I assume that it occurred when I was pouring the plates although it could occur anytime of course.

If you think you see a good leading edge 180° away from that satellite then take a wedge and put it on a clean plate now. Otherwise, it's far enough away that you probably have enough time to let your culture grow out a little bit so you can get a good wedge to transfer. The longer you wait however the higher risk you incur.


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nokumana] * 1
    #29604051 -

Looking closer, I think I see mycelium, so could be mold or fungus or it could be a satellite of your original culture if you weren't too careful about transferring it. How did you plate the original culture?


--------------------
-Noku
Eskawata kaya waikiki
HHG
"Shake it like you would a newborn baby" - BeefExtreme

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nokumana]
    #29604067 -

Nokumana said:
Looking closer, I think I see mycelium, so could be mold or fungus or it could be a satellite of your original culture if you weren't too careful about transferring it. How did you plate the original culture?






Poorly :lol: I started from spores and then tried to take some aerial mycelium to t-1. The satellite texture is different, looks like tv static. I doubt it's cube myc.

The yellow blob on the t-0 is interesting, I thought it was clumped malt extract but it seems to be growing.

I guess the take away is that something is wrong with my sterile work.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Turvenuija]
    #29604088 -

I’d be willing to bet it’s mycelium. This happens to me a lot kuz my tek isn’t perfect and I end up dragging it or dropping it in the plate I’m transferring to or have trouble getting it off the scalpel and that’s when it happens

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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