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Re: Why is air fryer not better than dehydrator? [Re: oktopus] * 2
    #29580379 -

I've never had a problem with air dried fruits during storage as long as you give them some gentle heat at the end. I've found those little silica gel packets just aren't up to the job though, so I make my own silica gel packs from colour change silica gel beads and emptied out tea bags. They change colour from orange to green when they're full so you can check your stored fruits for dryness without opening the jar.



So rather than fretting about cracker dryness and subjecting them to more heat just in case you can make the desiccant do the cracker drying.

Splitting or cutting the fruits before drying should definitely be avoided as splitting the fruits led to a 30% loss of potency compared to fruits dried whole in the research but it probably can't be avoided if you're growing the jumbo mutant strains which take an age to dry whole and probably won't respond so well to air drying.

To minimise potency loss during storage the jars should be dark glass or wrapped in foil to exclude all light and stored at room temperature in line with the published research which found that those conditions led to the least degradation over time. Freezing seemed to degrade potency, which likely occurs when they're brought back out into room temperature when any residual water content in the fruits that's frozen as ice goes through the phase transition from a solid back into a liquid. It's worth noting that the research only used sub zero and room temperature, with and without light, so refrigeration of the storage jars would likely help preserve potency too as long as the dried fruits don't freeze.

Vacuum sealing the storage jars to remove oxygen or replacing the air with an inert gas like nitrogen or CO2 will also slow down any loss of potency.

They're best stored whole as it reduces the surface area that's exposed to moisture, light and oxygen and all the alkaloids are stored under the outer skin which acts as a protective barrier when the fruits are fresh or dried. Capsules are likely the best way to store powdered fruits as once the powder is packed tight inside and the capsule is sealed the outer shell would give some protection against moisture and oxygen. Black capsules that exclude light should be standard.

Hope that helps!


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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett]
    #29581063 -

which research shows that cutting a fresh shroom leads to 30% loss?

what you said that storing whole is also not consistent to what some data says:

https://www.shroomery.org/forums/showflat.php/Number/28462347/fpart/1/vc/1

capsules shows a 0,54% of psylocibin loss in a year

meanwhile, other samples, in their dried whole state lost 6% in a year.

"OO Capsules were made with coffee grinder, and capsule maker in June 2023. The contain leftover pins/pieces/dust from a 2022 jar (~25%) and an even mix of blobs, Penis and regular fruits (~75%) from 2023. Kept in sealed mason jar with desiccant since prepared. Two capsules put aside for testing in 1 year."
Test results:
Psilocybin = 8.1 mg/g
Psilocin = 1.1 mg/g
Baeocystin = 0.3 mg/g
Total: 9.5 mg/g

----
SAMPLE 2023 Capsules
Aeruginascin = 0.033 mg/g
Baeocystin = 0.257 mg/g
Psilocin = 0.756 mg/g
Psilocybin = 8.056 mg/g
Total Alkaloids = 9.102 mg/g

%loss (9.5-9.102)/9.5 = 4.1% loss"(this is all alkaloids, not only psylocibin)

"#1 2020/2021 A
This sample contains a random mix of harvests from 2020 & 2021, multiple generations, some indoor, some outdoor, some selected clones, some CV, some CV+manure. I tried to get a reasonable representation of what was contained in the jar. Jar was sealed in 2021, opened maybe once or twice and then re-heat-sealed each time. Nesco 165F dried for 24 hours. I personally bioassayed 2.8 grams of the 2021 harvest and it was stronger than anticipated, but within the traditional cubensis range I had experienced before. Multiple 4 gram bioassays from multiple people showed traditional/expected cubensis level potency.
Test results:
Psilocybin = 4.6 mg/g
Psilocin = 0.6 mg/g
Baeocystin = 0.2 mg/g
Total: 5.4 mg/g

#2 2020/2021 B
This second sample contains a random mix of harvests from 2020 & 2021, multiple generations, some indoor, some outdoor, some selected clones, some CV, some CV+manure. I tried to get a second/different reasonable representation of what was contained in the jar. Jar was sealed in 2021, opened maybe once or twice and then re-heat-sealed each time. Nesco 165F dried for 24 hours.
Additional sample set aside for testing in 1 year
Test results:
Psilocybin = 4.2 mg/g
Psilocin = 0.6 mg/g
Baeocystin = 0.3 mg/g
Total: 5.1 mg/g

----
SAMPLE 2020/2021 C
Aeruginascin = 0.026 mg/g
Baeocystin = 0.247 mg/g
Psilocin = 0.407 mg/g
Psilocybin = 4.116 mg/g
Total Alkaloids = 4.796 mg/g

%loss = (5.25 (Average of previous 2 samples)- 4.796)/5.25 = 8.6% loss"


another data is this GordoTek table right here:




powdered shroom is better than whole shroom with and without argon or oxygen free environment.

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Re: Why is air fryer not better than dehydrator? [Re: Chiron098]
    #29581165 -

The research paper you cited in your OP that you mistakenly thought proved that heat doesn't degrade potency, "Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis" found that cutting or slicing fresh mushrooms led to a 30% loss of potency.

3.1.1

"On the contrary, the yields of the analytes from unprocessed fresh mushrooms in comparison with chopped fresh mushrooms were as follows: 33% increase in psilocybin, 37% increase in psilocin, 1.5% increase in baeocystin, and 12% increase in aeruginascin. The concentration of norbaeocystin was below the LOQ. Therefore, the unprocessed fresh mushrooms contained approximately 30% more tryptamines than the chopped fresh mushrooms".

And the question I was asked was, "What is your recommendation for home cultivators for long time storage?" How many people do you know with cylinders of argon kicking around at home making the place look untidy?

I covered removing oxygen from the storage jars with a vacuum sealer or replacing the air with an inert gas like nitrogen or CO2 as well as the degradation reducing effects that packing powdered fruits into capsules likely has in my post.

The paper you cited also looked into potency loss of powdered fruits during storage and this was the result.



Sure we can slow that down by various means but once mushrooms are harvested potency only goes one way and that's down.

And as mentioned in one the old threads you posted, there's more oxygen in a jar of dried fruits than a jar filled to the brim with powder which might result in less degradation during storage but you can remove the oxygen from your storage jar to get over that. It's also important to remember that long term storage for most people involves opening the jars every time they want to get dried fruits or powder out and powder absorbs moisture really quickly from the humidity in the air and has more surface area exposed to oxygen and light compared to whole dried fruits. So a sealed jar doesn't reflect the conditions that most people's stored fruits experience during normal use. And most people prefer to store their dried fruits whole.

If the potency losses during storage worries you you should also be concerned about the potency loss from using elevated temperature drying before storage which leads to way more potency loss in a short time that the slow reduction of potency that occurs during storage.

So the best advice regarding long term storage for most people is to reduce exposure to moisture, oxygen and light and use them as quickly as possible after drying and avoid storing them for long periods of time at all.


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Edited by Dave Bennett (05/20/26 03:47 AM)

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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett]
    #29581205 -

Btw, a vaccuum sealer totally seals polyfil batting.  We make little charcoal filter pouches for our cats fountain this way.  Just sayin.  Would make a clean desiccant pouch.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett]
    #29581211 -

Another factor, that was not mentioned in this thread, and for me personally is important is cost of drying. Running 420W dehydrator on high for 24h few times a month become a significant portion of my electric bill and sometimes, with smaller harvest I just don’t dry them at all. With Dave’s method it would be more energy efficient - for me it is worth trying. Of course everybody’s situation is different. I’m just going to test it for myself and see it that makes a difference.

Edited by oktopus (05/20/26 05:44 AM)

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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett]
    #29581337 -

before the quote you putted it has:

"We found that the dried fungal fruiting bodies had a better yield
from a fungal powder than from whole pieces. Conversely, in the case
of fresh fungal fruiting bodies, the extraction of larger unprocessed
pieces of fresh fruiting bodies was found to be more effective than
chopping these mushrooms into small pieces. The fresh fruiting bodies
quickly turned blue as they were cut into smaller pieces, which may
be due to the psilocybin hydrolyzing to psilocin, which then oxidizes
to quinoid dye."

thus, they chopped the shroom in small pieces, not cut lengthwise. thus this doesn't mean "cutting lengthwise will cause 30% loss", it means that chopping the shroom in small pieces will cause 30% loss for obvious reasons.

the point of argon is to create an oxygen free environment, and the table i showed powder win against whole shrooms BOTH WITH AND *WITHOUT* ARGON. thus this is totally doable to make at home, and using a vacuum is also the same as using argon.

and powder has LESS surface area, that's why it wins. if you compress it in the jar(anubis tek) the interstitial air gets removed(of course not entirely, but reduced by a lot), and only the surface air(headspace) acts on the shroom. meanwhile, whole shrooms are full or interstitial air.

to deal with humidity you use silica, as always, very standard and everyone does that anyway.

"So the best advice regarding long term storage for most people is to reduce exposure to moisture, oxygen and light and use them as quickly as possible after drying and avoid storing them for long periods of time at all."

is ironic that you say that dehydrating faster is better, but you also say to not use heat, even though is what most people have access to dehydrate faster. heated air flow is very cheap.

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Re: Why is air fryer not better than dehydrator? [Re: Chiron098]
    #29581363 -

Quibbling about the definition of a word isn't the flex you think it is when it comes to your attempts to try and disprove peer reviewed published research. I said "splitting or cutting" and it should be blindingly obvious to even newbies that triggering the blueing reaction on large inner surface areas of the fruits is going to cause a loss of potency. Where do you think the blue colour comes from?

And you seem really confused about what I'm saying. I said drying fruits slowly is better as it results in higher potency. If you don't understand what I'm saying or the basics of the psilocybin degradation processes how are you in any position to disprove what I'm saying and overturn five decades of research?


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Re: Why is air fryer not better than dehydrator? [Re: BeefExtreme]
    #29581423 -

That's a great tip! I've got a vac sealer so I'll definitely be trying that. The staples have been irritating me every time I make them or set eyes on them in the jars. You can't beat a professional looking finish.


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Re: Why is air fryer not better than dehydrator? [Re: Chiron098] * 1
    #29581457 -

There's a real irony in perpetually leaning on the phrase "peer reviewed" to deflect what is essentially peer review.

There's no such thing as "dealer shrooms," and there's also no such thing as a professor of "shroomology." Yeah, there are mycologists and biochemists and whatnot, but nobody in proper academia has dedicated their public professional life to the exact things we're talking about here.

And yet, with all that said, the process of selecting reviewers for scientific papers is also a lot more haphazard than people might imagine. Real peer review kinda resembles exactly what we're doing here.


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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett] * 1
    #29581464 -

cutting in half is very different than cutting in small pieces like the study does. thus your phrase saying "cutting leads to 30% loss" is actually wrong.

when you cut you don't expose the entire shroom to air, if it was, when you harvest theoretically would have no psylocibin anymore. only a small fraction of the shroom oxidize immediately, and of course, if you cut a lot, like in small pieces, this small fraction can actually become a bigger fraction.

the basics of psylocibin degradation is that psip will transform psylocibin into psilocin and psil will destroy the psilocin. when the shroom is intact and receiving nutrients from the mycelium, the psip and psil are not in contact with psylocibin, they are in different "rooms".

when you dehydrate slowly, the natural cell death(senescence) of the shroom and the own process of dehydration(removing water) will make the psip and psil enzymes come in contact with psylocibin. the cytosol, where psylocibin is, will shrink while the lysosomes(vacuole) where psip is, will lose the integrity and then both will come in contact.

that's why dehydrating faster and with heat is better. after psip and psil touches psylocibin, is only a matter of seconds to psylocibin turn into psilocin, then into nothing, proving by the blueing reaction that happens in seconds. this means that if you don't denature enzymes, the enzymes will degrade psylocibin MUCH faster than heat will degrade psylocibin.

as the own dehydration process, from removing the water from the cell, causes the psip to react with psylocibin, this means that if you don't kill the enzyme, EVERY dehydration process will have this enzyme problem, but if you use heat, the enzymes gets denatured (killed) and don't pose a problem anymore.

also, the enzymatic degradation occurs in a matter of seconds, meanwhile, the heat degradation takes several minutes to hours to make substantial loss.

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Re: Why is air fryer not better than dehydrator? [Re: Chiron098]
    #29581549 -

Have a look into the effects of elevated temperatures on vacuole membranes and you'll find out that their lipid layer is weakened by elevated temperatures. So when they're exposed to elevated temperatures and rapid water loss from the hyphal compartment they quickly lose their integrity and leak or burst and spill their contents out into the cytoplasm where PsiP turns psilocybin into psilocin. PsiL is predicted to be stored extracellularly on the outside of the hyphal wall which only allows the blueing reaction to occur if the hyphal wall is damaged or loses its integrity when it leaks it's psilocin rich cytoplasm outside the cell where it interacts with PsiL to produce the blueing reaction.

With slow drying the hyphae are still metabolically active while there's enough water remaining in the fruits, psilocybin and PsiP stay compartmentalised in their vacuoles much longer into the drying process and any psilocin produced in hyphal compartments is rephosphorylated back into psilocybin by PsiK. No enzymatic degradation can occur in undamaged hyphae as PsiL is on outside of the cell wall.

So if you denature the enzymes as soon as possible with elevated temperatures it leads to an increased loss of potency because you've just knocked out the repair enzyme that protects psilocybin from degrading during the drying process and you're making the vacuoles spill their contents when the water content of the cytoplasm is highest and psilocybin can easily be degraded by PsiP.

There's even evidence in the "Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis" paper you cited that the psilocybin content of the fruits increases during room temperature drying because all the synthesis enzymes are still active and producing psilocybin after the fruits have been harvested.

3.4 | Fresh versus dried fruiting bodies

"These experiments aimed to determine the water content in fresh mushrooms and to analyze the concentration of tryptamines in fresh and dried fruiting bodies, which were dried in the dark at room temperature."

"In conclusion, the concentrations of tryptamines were as follows: 0.54 wt.% of psilocybin, 0.02 wt.% of norbaeocystin, 0.01 wt.% of aeruginascin, 0.05 wt.% of baeocystin, and 0.25 wt.% of psilocin. Within the experiments with homogeneous dried fungal powder, the concentrations of tryptamines were as follows: 0.62 wt.% of psilocybin, 0.02 wt.% of norbaeocystin, 0.01 wt.% of aeruginascin, 0.06 wt.% of baeocystin, and 0.16 wt.% of psilocin."

I got Grok to put those figures into a chart.



"In summary, the average total content of tryptamines in fresh and dried mushrooms was approximately 0.87 wt.% in both cases. This is confirmed by studies that claim that the tryptamine content in fungal biomass is not reduced by drying in the dark at room temperature."

"Generally, there were higher concentrations of all analytes except for psilocin in dried mushroom powder. We found a 27% increase in norbaeocystin, a 12% increase in aeruginascin, a 26% increase in baeocystin, and an 18% increase in psilocybin in comparison to the fresh mushrooms."

"Tryptamines in fresh fruiting bodies may act enzymatically by kinases before the fruiting body is dried, as in the case of phosphorylating psilocin to psilocybin."

So in this experiment there was very little statistical difference between the potency of fresh fruits and room temperature dried fruits, proving that potency degrades less during low temperature drying compared to the increasing potency losses which occurred when psilocybin is exposed to increasing temperatures as shown in this chart.



And the findings concur with the chart from this paper that looked into drying temperatures and potency.

Forensic analysis of hallucinogenic mushrooms and khat (Catha edulisForsk) using cation-exchange liquid chromatography.



You'll notice that the alkaloid content of fruits dried at room temperature is higher than the "fresh calculated as dry weight" figures, again indicating that the psilocybin content is increasing during slow room temperature drying.

And just to bring things right up to date, the latest Psilocybe paper to published a week or so ago states,

"Conversely, when a high content of water was used in the extraction solvent, along with higher temperatures and the presence of alkaline agents, a decrease in the concentration of the target compound was observed. This is attributed to the reactivity of the molecule, which undergoes degradation when exposed to high humidity, elevated temperatures, and atmospheric oxygen, leading to the formation of blue-coloured derivatives."

So that's yet another paper to add to the scientific consensus that heat degrades potency. I take it you still haven't found a shred of scientific evidence that supports elevated temperature drying, yet?


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Edited by Dave Bennett (05/20/26 02:12 PM)

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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett] * 2
    #29581673 -

Yeah check it out.  Heres a packet we made.

.

Theyre clean. 
Betting it will seal tyvek too.  Just saying. 

Anyway man.  Its not a new concept by any means and most of us are well aware that active alkaloids decay with heat.  The data is meaningless to me in that sense.  It tells me what we have always known.  We just dont care and I hope to explain to you why we dont really care:

Air drying CAN work.  Not one TC will argue that it cant.  Does it preserve the alkaloid profile better?  Probably.  We dont care.

In a dry climate, ive done exactly what youre suggesting.  You can pretty much use a fan to get things 'mostly' dry but youre always going to require some heat to get it completely dry for safe long term storage. 

The issue with a technicque that CAN work is that it cant be applied to every climate.  I wouldnt try this on the coast of maine, for example.  The result would catastrophic oxidation. 

So we recommend people use a dehydrator because it DOES work. In all climates.  Its a highly effective way of retaining MOST of the actives for a vast majority of the growers world-wide.  We are aware that some alkaloids are lost in the process, and always have been.  Our answer to this has always been grow more, eat more.  Like i said, even if i lost half the actives drying yeti, 2g will still turn you inside out.  Again.  We dont care. 

Please try to understand that our purpose and intent with this community is to offer the most reliable advice we can.  There is no reliable way to tell the world to air dry their mushrooms at this time that i am aware of, so convention remains with dehydrators. 

If you intend to bend the the ray of convention, you have to provide much more than peer reviewed data.  We are in the business of collating techniques that can be universally applied with consistent results.  I dont see in my minds eye an air drying technique that would work in high humidity climates, but of course Im open to listening, provided you can illustrate your ideas without acting like everyone except for you is stupid. 


Does that make sense? Totally not trying come at you slanted, im just trying to show you our perspective.
Yes heat is one factor that contributes to decay.  We know.  Does that mean we stop recommending people use dehydrators to preserve their mushrooms? Absolutely not. 


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

Edited by BeefExtreme (05/20/26 03:21 PM)

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Re: Why is air fryer not better than dehydrator? [Re: BeefExtreme] * 1
    #29581691 -

But also, full circle, we arent going to be recommending air friers either.  Thats bonkers.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: Why is air fryer not better than dehydrator? [Re: BeefExtreme]
    #29581713 -

Even air fryers with dehydration setting that can go below  100 degrees?

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Re: Why is air fryer not better than dehydrator? [Re: Awfullotofshrooms]
    #29581717 -

It wont be in the hitchhikers guide any time soon.  I wont say it wont work but i wont be publishing it either.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett]
    #29581878 -

now we are in a total awkward situation.

PsiK enzyme doesn't work without ATP, what means that after harvesting the shroom, theoretically the PsiK stop getting energy(ATP) to work. the energy that remains in the shroom of course don't last 12-24 hours or more.

thus, now we have an dilemma here:

or the scientists had a professional method that could dry the shroom very fast without using temperature, so ATP and Psik enzyme was still working(what is not easy to replicate at home anyway, so no practical use)

or the extraction of fresh shrooms was actually trash. I've seen that to extract alkaloids from fresh shrooms, using a whole fresh shroom don't work, because it works like a sponge and takes much time to actually extract everything. and if you cut, squeeze, or anything like that, the psylocibin will oxidize instantly, becoming blue, as this is a injure. thus, in this case, it would not be that the dried shroom increased in psylocibin, but that the fresh shroom DECREASED in psylocibin by the extraction method.

the math of the alkaloids also don't math, as psylocibin weights more than psilocin. saying that both have a 0,87 wt% is strange, as the dried one should have a higher percentage as have more psylocibin.

long story short, this result actually don't matter and can't prove nothing unfortunately.

and also, your table in the study "Forensic analysis of hallucinogenic mushrooms and khat (Catha edulisForsk) using cation-exchange liquid chromatography." is not good and shouldn't be used for anything. the freeze drying has THREE TIMES more alkaloids than the fresh shroom. this is ridiculous. the psik wouldn't do that much, even if it was doing the rephosphorylation. also, psik functions making psilocin back do psylocibin, thus the 20C dried shrooms should have LESS psilocin, not more.

you also doesn't seem to realize that i never said that heat does nothing to degradation. what i said is that HEAT DEGRADE *LESS* THAN ENZYMES, specifically psip and psil.

even though, I've found your argument very interesting, and i would agree if you post more data supporting for how long the ATP takes to be fully used in harvested shrooms. with this data, we could deduce how much would psik last and protect psylocibin from psip and psil.

Edited by Chiron098 (05/20/26 07:04 PM)

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Re: Why is air fryer not better than dehydrator? [Re: BeefExtreme] * 1
    #29582216 -

Who said anything about not using a dehydrator? The debate is about the negative effects of temperature on potency. Research has proven that low temperature drying results in the highest potency and the recommendation from the research for people using dehydrators is to dry the fruits below 40C (104F) to prevent an unnecessary and completely avoidable loss of potency.

What ever the drying method, drying below 40C is good advice. Drying above 40C is bad advice. That's all I'm saying in a nutshell.

And isn't high potency fruits a common goal that every cultivator aspires to? High potency strains are revered and prized in the community, people enter high potency cup competitions where a fraction of a percent difference in alkaloid content can make you winner or a loser, drying temperatures affect alkaloid ratios, the subjective effects of the trip, degradation during storage and shelf life, the list goes on. I think most cultivators care deeply about the potency of their fruits.

Regarding the feasibility of air drying and climate, if I can successfully air dry my fruits in a temperate maritime climate anyone outside the tropics can probably do the same. Obviously the lower the atmospheric humidity the better but fruits will still lose water as long as the relative humidity of the air is below 100%. Even in relatively high humidity a simple fan constantly delivers a flow of dryer air which increases the rate of evaporation from the fruits.

I'm not trying to make air drying compulsory or change any rule books, I'm just giving people vital information on the subject that allows them to make an informed decision about their drying methods. If people still want to go full blast in a dehydrator be my guest.


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Edited by Dave Bennett (05/21/26 03:32 AM)

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Re: Why is air fryer not better than dehydrator? [Re: BeefExtreme]
    #29582219 -

Wow, how interesting!
If I live in a dry climate, could I use a fan to dry them to 90% and then finish drying them with a dehydrator, to achieve better results in terms of potency and preservation of active compounds than using only a dehydrator?


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- A Dreamer in the Nightmare of being Awake -


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Re: Why is air fryer not better than dehydrator? [Re: Dave Bennett] * 1
    #29582221 -

I think what most of us is wondering is if it's better to dry them below 40°C even if that takes 4 days as opposed to do 20 hours at 70°C :strokebeard:

I have been running me dehydrator for 24 hours at 40°C(lowest setting) now and my Papuana still isn't cracker dry, so when I(hopefully) harvest the Yeti's in about 2 weeks I wonder how many days it would take to get them to cracker dry :confused2:

I just skimmed most of the papers, but I couldn't see that any of them tried drying whole fruits at different temperatures with a timeline, so I guess that is why this is even a debate, could be wrong though, just had my morning crayons :muppet:


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“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Why is air fryer not better than dehydrator? [Re: Chiron098]
    #29582224 -

Mushrooms are living fungal tissue. After harvest, they remain metabolically active until they dry out, spoil, or are processed and they continue cellular respiration to generate ATP using stored reserves (glycogen, sugars, etc.) from the fruiting body for hours or even days depending on temperature and atmospheric humidity.

New preservation and detection technologies for edible mushrooms: A review

"Edible mushrooms are nutritious, tasty, and have medicinal value, which makes them very popular. Fresh mushrooms have a high water content and a crisp texture. They demonstrate strong metabolic activity after harvesting."

So ATP can be manufactured by the fruits after harvesting to fuel psilocybin synthesis.

Regarding your confusion about this chart.



They tested 100mg of each material.

100mg of fresh material (90% water) had a total psilocybin content of 0.039mg. That's a measured analytical figure.

100mg of the  freeze dried material had a total psilocybin content of 0.858mg. That's a measured analytical figure showing the true potency of the dried material without any or minimal degradation caused by the drying process and forms the control to measure the loss of potency caused by drying at room temperature and 60C.

100 mg of 20 to 25C dried material had a total psilocybin content of 0.496mg. This is a measured analytical figure and by comparing it to the freeze dried figure you can work out the loss of potency caused by room temperature drying.

100mg of the 60C dried material had a total psilocybin content of 0.086mg. This is a measured analytical figure and by comparing it to the freeze dried figure you can work out the loss of potency caused by drying at 60C.

The fresh calculated on dry weight figure, however, is a hypothetical figure calculated by working out how many mg of psilocybin 100 mg of the fresh material would contain if all the water content was removed using this formula, Dry = Wet Ă— (100 / % dry matter).

The hypothetical fresh calculated on dry weight figure is 0.353mg. The actual figure for 20 to 25C drying is higher than the hypothetical dry weight figure indicating that potency has increased when the fruits are dried at room temperature.

For potency to increase the psilocybin synthesis enzymes have to still be active and producing psilocybin after the fruits have been harvested. And we know from the research that fruits can produce ATP and enzymes are still active in harvested fruits which likely explains the increase in potency during the room temperature drying process.

Hope that was easy enough to follow. Let me know if there's anything I haven't explained to your satisfaction.


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Edited by Dave Bennett (05/21/26 04:13 AM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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