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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean] * 2
    #29518745 -

Sulfurshelfsean said:
Took some jars out while they were still hot to shake them and there was still a vacuum pulling on the jars through the filters. Can this vacuum suck in contams? I just thought about it, the ragsbibwas using to hold the hot jars were kinda funky, lack of planning on my part.


For what it's worth i've shaken freshly PCd millet jars a number of times without contamination issues from them still being hot. I use 2 part lids with sticker filters off zon

Guess it depends on the filters, but I'd bet your jars will be fine :cool::mushroom2:

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge] * 1
    #29518747 -

Just a thing I had seen someone post on here and then I can label the one bag instead of 5 individual swab wrappers.

Plus, I like to keep the swabs in a half gallon mason with a dry pack


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge] * 2
    #29518790 -

Agar Wedge said:
Sulfurshelfsean said:
Took some jars out while they were still hot to shake them and there was still a vacuum pulling on the jars through the filters. Can this vacuum suck in contams? I just thought about it, the ragsbibwas using to hold the hot jars were kinda funky, lack of planning on my part.


For what it's worth i've shaken freshly PCd millet jars a number of times without contamination issues from them still being hot. I use 2 part lids with sticker filters off zon

Guess it depends on the filters, but I'd bet your jars will be fine :cool::mushroom2:


I was more worried about the rags I grabbed to shake them with, they were kinda funky. Could bacteria get sucked through the filter from the vacuum that was created?


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean] * 1
    #29518883 -

Nah.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog]
    #29519003 -

What's everyone's thoughts on this?



--------------------
The Hitch Hiker's Guide

Bod's TEKs

Pastywhyte's Easy Writeups

Baba's TEKs (Exotics and more)

callmehippie's Extensive List of Everything

Anything and Everything Else You Will Ever Need

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Re: Ask Quick Questions, Get Quick Answers [Re: RhyzoPhreak] * 2
    #29519016 -

RhyzoPhreak said:
What's everyone's thoughts on this?




Frustrating.


--------------------
🌾💮🫘🥖

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Re: Ask Quick Questions, Get Quick Answers [Re: RhyzoPhreak] * 4
    #29519028 -

RhyzoPhreak said:
What's everyone's thoughts on this?




I bought a couple of gadgets from mush cult places. None of them were worth beans. The best thing I have is my curved aquarium scissors. By far the best thing I’ve used and it is sharp and easy and I can literally snip a single pan from between two pins without disturbing the pins. And I can get under fat blobs of yeti and get them out without tearing up the substrate. This is the one I have and I love it!


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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29519289 -

7 days. no pins yet. Got an light pointed at the thing, I fan daily


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Edited by brominegeek93 (03/18/26 07:46 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112]
    #29519334 -

AZ Starman2112 said:
RhyzoPhreak said:
What's everyone's thoughts on this?




I bought a couple of gadgets from mush cult places. None of them were worth beans. The best thing I have is my curved aquarium scissors. By far the best thing I’ve used and it is sharp and easy and I can literally snip a single pan from between two pins without disturbing the pins. And I can get under fat blobs of yeti and get them out without tearing up the substrate. This is the one I have and I love it!





The stuff from mush shops is definetly gimmicky and would agree. It’s either 1) overpriced when you can find it for cheaper (scalpels & blades) or 2) cheap crap that will break instantly but sounds like a needed cult cause it’s a “problem” you haven’t “thought about yet” and will “make your life easier.


My blade of choice.



--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29519343 -

brominegeek93 said:
7 days. no pins yet. Got an light pointed at the thing, I fan daily


Patience. I have MVP box, that knoted after 2 months. If everything is ok, then it will pin eventualy.

You can also show some pics, mayby something isnt ok :ohwell:


--------------------
.
"How do you eat an elephant? One bite at a time."

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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29519353 -

I have questions:

You’re working in a still air box.

1. What do you do with the syringe when you need to wipe down the entire jar before inoculating it?

You get a syringe where you need to undo that plastic cap and put on a new needle.

2. Do you wipe the outside of the sterile needle with alcohol prior to opening it and putting it on the end of the syringe?

3. Is this done inside of the glove box?

4. Is an alcohol soaked paper towel as good as a disposable alcohol prep pad?

5. If you set the alcohol soaked paper towel on the table inside of the still air box, is it still sterile or is there a chance for contamination?

6. Do you flame sterilize between jars, or only if the end of the needle touches something that’s not a jar?

7. Is it okay to flame sterilize and then cool the end of the needle off with an alcohol soaked paper towel?

8. Do you use a new roll of paper towel for each inoculation session?

9. Is it better to bleach the inside of the still air box, or is spraying it down with 70% iso okay?

I really fucked up yesterday and started a small fire. I was going ham on the alcohol and it ignited in the SAB and I freaked out. I’m just a mess. I looked through lots of threads about inoculation and used different key words in the search, and I couldn’t figure out exactly what to search for when I was looking around. The best info I got was from the thread on pf tek, but it left me with a lot of questions. Thank you all so much for your help ahead of time!

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Re: Ask Quick Questions, Get Quick Answers [Re: SexxLaws] * 1
    #29519363 -

SexxLaws said:
I have questions:

You’re working in a still air box.

1. What do you do with the syringe when you need to wipe down the entire jar before inoculating it?


I wipe down my jars first, or just spray them down, inside the box.



You get a syringe where you need to undo that plastic cap and put on a new needle.

2. Do you wipe the outside of the sterile needle with alcohol prior to opening it and putting it on the end of the syringe?


Wipe the plastic part down, flame sterilize needle outside box


4. Is an alcohol soaked paper towel as good as a disposable alcohol prep pad?

Yes



5. If you set the alcohol soaked paper towel on the table inside of the still air box, is it still sterile or is there a chance for contamination?


Overthinking, just act fast


6. Do you flame sterilize between jars, or only if the end of the needle touches something that’s not a jar?

Between jars



7. Is it okay to flame sterilize and then cool the end of the needle off with an alcohol soaked paper towel?


No!  And basically the first little bit of liquid cools it so don't wait around cooling



8. Do you use a new roll of paper towel for each inoculation session?


Flame sterilize the needle. All the jars, just wipe them down once while inside the box



9. Is it better to bleach the inside of the still air box, or is spraying it down with 70% iso okay?


Soap or 70 iso.  Bleach would be terrible to breathe...




Answers in the quote


--------------------
The closer you get to the meaning the sooner you know that you're dreaming

How the Bushman gets stuff done

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Re: Ask Quick Questions, Get Quick Answers [Re: SexxLaws] * 3
    #29519376 -

SexxLaws said:
I have questions:

You’re working in a still air box.

1. What do you do with the syringe when you need to wipe down the entire jar before inoculating it?

You get a syringe where you need to undo that plastic cap and put on a new needle.

2. Do you wipe the outside of the sterile needle with alcohol prior to opening it and putting it on the end of the syringe?

3. Is this done inside of the glove box?

4. Is an alcohol soaked paper towel as good as a disposable alcohol prep pad?

5. If you set the alcohol soaked paper towel on the table inside of the still air box, is it still sterile or is there a chance for contamination?

6. Do you flame sterilize between jars, or only if the end of the needle touches something that’s not a jar?

7. Is it okay to flame sterilize and then cool the end of the needle off with an alcohol soaked paper towel?

8. Do you use a new roll of paper towel for each inoculation session?

9. Is it better to bleach the inside of the still air box, or is spraying it down with 70% iso okay?

I really fucked up yesterday and started a small fire. I was going ham on the alcohol and it ignited in the SAB and I freaked out. I’m just a mess. I looked through lots of threads about inoculation and used different key words in the search, and I couldn’t figure out exactly what to search for when I was looking around. The best info I got was from the thread on pf tek, but it left me with a lot of questions. Thank you all so much for your help ahead of time!


Wow. Dude. You’re a hot mess! 😂

I see you have been around a couple years but don’t have a lot of posts. I’ll try to give you some answers to all your questions and hopefully help you in the right direction.

So rule number one…never work with an open flame inside the SAB. Hard rule. It causes air currents. Add alcohol in a closed space and you literally now have a bomb. Thankfully you didn’t get injured. But no worries, we are all here for you and will help you!

You can wipe down the jar outside the SAB and carefully place it inside. You can prep the needle outside the SAB. It’s a sterile needle. Open the end of the needle that slips on the syringe. Wipe the top of the syringe with an alcohol wipe, remove cap in syringe, and wipe again. Slip the needle on and twist. Remove wrapper. Remove needle cap, flame sterilize it outside the SAB. Slowly move it into the SAB. A couple of drops through the needle will cool it down. Do not wipe the needle as it is sterile at this point. You can place it down just don’t let the needle touch anything. Wipe your injection port with an alcohol wipe and then inject the jar. You can use a paper towel dampened with alcohol, I do all the time. This cheaper than alcohol wipes, but not sterile as alcohol wipes are until you open them and touch them. You do not want to wipe a hot needle with alcohol, one for flammability, two because you’ll taint its sterility. You do not need to use a new roll of paper towels each time, but a dedicated roll for cult work is a good idea. Always flame sterilize the needle between inoculations. You can use bleach or alcohol to clean. I personally use alcohol because I absolutely despise the smell of bleach. Also alcohol and bleach do not sterilize, they sanitize. Big difference, so that should answer the question about setting down your alcohol dampened paper towels in your SAB. Remember your SAB is only a sanitized environment with minimal air currents and closed off from the room you are in blocking stuff from falling into what you are working with. Developing smooth steady work flows and not working above your cultures inside the SAB is what you should aim for. Meanwhile keep that flame out of your SAB and be safe.

Also, you’ll probably hear this from others, if you are shooting spores directly into grain jars, that is one sure fire way to likely end up with contamination in your jars. You’ll get lucky from time to time and get a few good jars and a few good grows, but not easily and not without failing. I know because I started just that way. I came here and folks quickly told me that and pointed me to putting my spores on agar, getting clean cultures and then agar to grain and grain to bulk. Think about going that route as soon as you can. Also read the Hitchhikers Guide to The Shroomery if you haven’t. There is a lot of great info in there. The link is in my signature.

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Re: Ask Quick Questions, Get Quick Answers [Re: Blizzloo] * 1
    #29519389 -


Except for a couple of bare spots it looks ok to me


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: AZ Starman2112] * 1
    #29519419 -

AZ Starman2112 said:
RhyzoPhreak said:
What's everyone's thoughts on this?




I bought a couple of gadgets from mush cult places. None of them were worth beans. The best thing I have is my curved aquarium scissors. By far the best thing I’ve used and it is sharp and easy and I can literally snip a single pan from between two pins without disturbing the pins. And I can get under fat blobs of yeti and get them out without tearing up the substrate. This is the one I have and I love it!




I also use these scissors.  Or ones similar.

The other tool just looks like a dumb gimmick.  Like 90% of stuff marketed to mushroom hobbyists.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: Ask Quick Questions, Get Quick Answers [Re: tree frog] * 1
    #29519480 -

Kinda reminds me of a pooper scooper


--------------------
Favorite Teks

  ]

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Re: Ask Quick Questions, Get Quick Answers [Re: SexxLaws]
    #29519555 -

Get/"build" an induction sterilizer or use bleach as to not put your SAB on fire


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29519571 -

Why use bleach when soapy water works just as well?

Needing to sanitize your SAB at all hints at a misunderstanding of the purpose of a SAB.

99% of the time I run mine dry. As long as I'm  not working on a pile of mold it usually works just fine. I do wash mine out with soap and water once a month.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon] * 1
    #29519581 -

Received LC from magic mushrooms which is a trusted vendor here.
I got only a few syringes so dont want to waste.

Should I test it on agar first or is it ok to expand it?
And should i leave the needle on or should attach a new stopper on the syringe?

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Re: Ask Quick Questions, Get Quick Answers [Re: Sehrmush] * 1
    #29519598 -

I dont use a SAB


--------------------
The PF Tek is my area.:oldman:

Where all anonymity is lost, there cannot exist a person.

Where one cannot avoid to share, there is God

www.zamnesia.nl
RustyWhyte is Commercial!



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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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