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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Splittinghairs]
    #29514356 -

ok perfect. ac is always on but now the temp is almost perfect without it so it doesn't turn of often. i put in the same room new tubs every couple of days , straight with polyfill, thats why the dust worried me

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Re: Ask Quick Questions, Get Quick Answers [Re: BagShaker] * 1
    #29514370 -

Get an air purifier, if you don't already have one....

Not for the mushrooms, but for yourself & Fam. All the in door contaminates we breathe-in is......... & only gonna get worse with cult.

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Re: Ask Quick Questions, Get Quick Answers [Re: BagShaker]
    #29514376 -

Hey folks, so I am going to buy a 10-pack of pre-made agar plates from a supplier because I am not set up to make my own. In the UK... I tried an Amazon supplier last time, and it wasn't ideal (one plate arrived contaminated and my success rate was low)

Now I am looking at a more specialist lab biologicals supplier ( if anyone knows a good one in the UK please PM me) and they have "Malt Agar"


and "Nutrient Agar", no ingredients listed


The Nutrient Agar looks like a lighter color and costs quite a bit more . (10-pack is £19 vs £14). Which one to get??  I see that people favour lower nutrients in the agar so the lighter type seems preferable?

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Re: Ask Quick Questions, Get Quick Answers [Re: Elixirel]
    #29514379 -

How are you going to use them, if you're not setup for agar, all you need is  SAB and Pc (plus agar).

Don't My  even need plates, my first few years only did pre-pours in small wide mouth. 4oz jars.


Edited by LewDoja (03/13/26 09:00 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja]
    #29514380 -

I have a SAB but no time or space for preparing agar, no pressure cooker, and no funds to buy one for a while.  I am resigned to spend a bit more on premade plates to do some agar to agar transfers and not waste time.

Could that "nutrient agar" be PDA? That wouldn't be more expensive  though , right?

Edited by Elixirel (03/13/26 09:08 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Elixirel] * 1
    #29514387 -



Why do we let APEs get marshmallow soft before we harvest them? Are they less potent if you harvest them while they're still hard? It's a bit annoying selectively harvesting like this ... Not sure how necessary it is?

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Re: Ask Quick Questions, Get Quick Answers [Re: Kunta Kente]
    #29514402 -

My bad. here are some better pictures.
Your growth patterns look very different - much more fluffy.
Mine has a very thin, sometimes almost cobweb like growth, sometimes hard to differentiate from white mold. But it doesn't smell modly.
This is supposed to be Natalensis btw. But someone here said it's propably a different culture since natalensis was misidentified as something else a couple of years ago. I guess we'll know when it's fruiting.
How often would you fan it?





Here are some more high res pics:
https://imgur.com/a/pJVRyBE


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Edited by brominegeek93 (03/13/26 10:02 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29514436 -

WTF is the dark color on the bottom of this plate? I have suspected it is some kind of mold or contam. This is T3 trying to get rid of it. The circled part is where I took a transfer to T4 trying to get it clean. There is also some trype of contam opposite where I took the transfer. TIA

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Re: Ask Quick Questions, Get Quick Answers [Re: Elixirel] * 2
    #29514451 -

Elixirel said:
I have a SAB but no time or space for preparing agar, no pressure cooker, and no funds to buy one for a while.  I am resigned to spend a bit more on premade plates to do some agar to agar transfers and not waste time.

Could that "nutrient agar" be PDA? That wouldn't be more expensive  though , right?


The only people who could really say for sure is the seller. Why not just send them an email?

Some prepared plates have recipes designed for cultivating bacteria and don't work well for mushrooms.


--------------------
I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Ask Quick Questions, Get Quick Answers [Re: PuffM4gicDr4gon]
    #29514480 -



Is this a good wild bird seed for grain spawn?

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo] * 1
    #29514500 -

Caffeineo said:
WTF is the dark color on the bottom of this plate? I have suspected it is some kind of mold or contam. This is T3 trying to get rid of it. The circled part is where I took a transfer to T4 trying to get it clean. There is also some trype of contam opposite where I took the transfer. TIA



That doesn't look like any healthy plate I've ever cultivated.  Thicker mycelium can look darker when backlit, but that doesn't seem like the case here?


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc]
    #29514501 -

That is what I have been thinking but it seems almost impossible to get it cleaned up as it goes with all the mycelium growth. :frown:

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo]
    #29514504 -

I don't see any cube mycelium in that photo if that's supposed to be a cube.

Not that it's a great photo.

But you might be transferring a contaminant mold or even a contaminate culture of some other mushroom.

It looks more like reishi or something along those lines.


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (03/13/26 11:55 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: Caffeineo] * 1
    #29514506 -

Is it possibly not mushroom mycelium to begin with?  Did you start from spores?

What does the top side look like?


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

Edited by andymc (03/13/26 11:58 AM)

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Re: Ask Quick Questions, Get Quick Answers [Re: LewDoja] * 1
    #29514514 -

LewDoja said:
Im team modified shoebox, and can always tape up the holes more if needed

Also the staggering of elevation of the holes create covection currents allowing passive evaporation whilst not over drying.

Its a balancing act, think about FAE... as Fresh Air & Evaporation (balance)


Lew in da house? Good to see ya, homie!

Anything specific to how you're modding your shoeboxes? I halfass kicked the idea around myself and was gonna wing it cuz like ya said - tape handles those happy little mistakes.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93]
    #29514526 -

added too much water to my agar mix, so it didn't get a solid consistency. I tried using a hair drier to dissipate some water but it just turned into a mushy mess. Is there any chance these plates will turn into solid gel blocks or should I scrap it and start again?



--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Elixirel]
    #29514534 -

Elixirel said:
Hey folks, so I am going to buy a 10-pack of pre-made agar plates from a supplier because I am not set up to make my own. In the UK... I tried an Amazon supplier last time, and it wasn't ideal (one plate arrived contaminated and my success rate was low)

Now I am looking at a more specialist lab biologicals supplier ( if anyone knows a good one in the UK please PM me) and they have "Malt Agar"


and "Nutrient Agar", no ingredients listed


The Nutrient Agar looks like a lighter color and costs quite a bit more . (10-pack is £19 vs £14). Which one to get??  I see that people favour lower nutrients in the agar so the lighter type seems preferable?


Ask the supplier. Lab suppliers will stock many different types of agar to grow and differentiate different organisms. It looks like TSA, which will grow fungi, but can’t be sure just from the colour.

TSA - Tryptic Soy Agar


--------------------
Method for Small Scale Casing Pasteurization

How much really fits in a 00 capsule?

Micro and Macro Mushroom Photography

Can you freeze mycelium?


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Re: Ask Quick Questions, Get Quick Answers [Re: brominegeek93] * 1
    #29514549 -

brominegeek93 said:
added too much water to my agar mix, so it didn't get a solid consistency. I tried using a hair drier to dissipate some water but it just turned into a mushy mess. Is there any chance these plates will turn into solid gel blocks or should I scrap it and start again?



You blew a hair dryer at your plates?  While they were exposed?  I assume this is pre-sterilization :wink:

Anyway, I'd suggest starting again, and probably put the hair dryer away too :lol:


--------------------
How I make spore prints
FFU build

"The idiots are taking have taken over" -NOFX

Edited by andymc (03/13/26 12:44 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: andymc]
    #29514550 -

Yes, no pour technique ofc.


--------------------
When my bird was looking at my computer monitor, I thought, "that bird has no idea what he's looking at." And yet, what does the bird do? Does he panic? No, he can't really panic, he just does the best he can... You’re that bird looking at the monitor, and you’re thinking to yourself, ‘I can figure this out.’ Maybe you have some bird ideas. Maybe that’s the best you can do.

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Elixirel] * 2
    #29514551 -

Elixirel said:
I have a SAB but no time or space for preparing agar, no pressure cooker, and no funds to buy one for a while.  I am resigned to spend a bit more on premade plates to do some agar to agar transfers and not waste time.

Could that "nutrient agar" be PDA? That wouldn't be more expensive  though , right?


Instapot works just as well.


--------------------
Favorite Teks

  ]

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Shop: North Spore Injection Grain Bag   Myyco.com APE Liquid Culture For Sale   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Substrate Bags   Original Sensible Seeds Autoflowering Cannabis Seeds

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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