Foreighn2891 said:

tree frog said:

About like this.

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Hi. Is the reason you placed 3 colonies is to avoid getting haploid mycelium?

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No in this instance these are actually all dikaryons already (bisporus).  I placed them on one plate because I don't like going through a lot of plates.  But also because it allows me to observe all three cultures together.

It's like those "One of these things is not like the other pictures" , if all of those pictures are closer together it's easier to spot when something is wrong.  But all three of these looked right, so I inoculated a jar with each one.

Anyway, it's the one of these things is not like the other was why I suggested ansr take three to one plate.  That and to save space.



This plate however I was making sure that I got a dikaryon.



And this is a close up of two strains on that plate crossing.

Edit:  And notice when I'm doing that, I put the wedges closer to the center so they touch sooner.  It's likely all three of those were already dikaryons because of how late they crossed, and the cross at the end was parasexual.

tultedspoon said:

...Go try it with moisture control soil and let me know if things turn out good for you. I had contamination show up much quicker and things growing from the soil that I never had an issue with when untreated. Everyone's environment is different on here.

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tree frog said:

Foreighn2891 said:

tree frog said:

About like this.

---

Hi. Is the reason you placed 3 colonies is to avoid getting haploid mycelium?

---

No in this instance these are actually all dikaryons already (bisporus).  I placed them on one plate because I don't like going through a lot of plates.  But also because it allows me to observe all three cultures together.

It's like those "One of these things is not like the other pictures" , if all of those pictures are closer together it's easier to spot when something is wrong.  But all three of these looked right, so I inoculated a jar with each one.

Anyway, it's the one of these things is not like the other was why I suggested ansr take three to one plate.  That and to save space.



This plate however I was making sure that I got a dikaryon.



And this is a close up of two strains on that plate crossing.

Edit:  And notice when I'm doing that, I put the wedges closer to the center so they touch sooner.  It's likely all three of those were already dikaryons because of how late they crossed, and the cross at the end was parasexual.

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Generic said:

so i've been tuning in but not reading up much scientific literature on mushroom sex, but i think i'm beginning to understand.  could y'all correct me where i'm wrong or mis-phrasing?


spores are usually haploid, which germinate into monokaryons. 

in the case of Panaeolus bisporus, the spores are dikaryotic(n+n), which germinate into dikaryons, and there are two spores per basidia, does that mean (n+n) & (n+n) on each basidia?
in this case, when do they mate?  do they live as dikaryons since birth, then fuse to form diploids upon mushroom production?

in the case of Panaeolus cyanescens, the spores are haploid (n), which germinate into monokaryons, and there are four spores per basidia, so is that (n) & (n) & (n) & (n) on each basidia?
when these germinate, they form monokaryons and promptly mate to form dikaryons, living their lives as dikaryons, and only becoming diploid upon mushroom production? 

(this is all omitting the para-sexual cycle)

and the difference between dikaryotic and diploid is... maybe dikaryons (n+n) are two different nuclei in the same cell, and diploid is maybe (nn)in one nucleus? in the same cell?

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tree frog said:

Foreighn2891 said:

tree frog said:

About like this.

---

Hi. Is the reason you placed 3 colonies is to avoid getting haploid mycelium?

---

No in this instance these are actually all dikaryons already (bisporus).  I placed them on one plate because I don't like going through a lot of plates.  But also because it allows me to observe all three cultures together.

It's like those "One of these things is not like the other pictures" , if all of those pictures are closer together it's easier to spot when something is wrong.  But all three of these looked right, so I inoculated a jar with each one.

Anyway, it's the one of these things is not like the other was why I suggested ansr take three to one plate.  That and to save space.



This plate however I was making sure that I got a dikaryon.



And this is a close up of two strains on that plate crossing.

Edit:  And notice when I'm doing that, I put the wedges closer to the center so they touch sooner.  It's likely all three of those were already dikaryons because of how late they crossed, and the cross at the end was parasexual.

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my MIB redspore got a birthday today

Generic said:

in the case of Panaeolus bisporus, the spores are dikaryotic(n+n), which germinate into dikaryons, and there are two spores per basidia, does that mean (n+n) & (n+n) on each basidia?
in this case, when do they mate?  do they live as dikaryons since birth, then fuse to form diploids upon mushroom production?

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They're pseudo-homothallic.  So some spores will have mating types that don't match and some will have mating types that do.  As an example, you need A and B to make a mushroom.  Some spores will have AA nuclei, some BB, and some AB.  The AA types will outcross with the BB types.

And very occassionally bisporus will have four spored basidia with a single nuclei.  But I have never observed one

The question of when they mate isn't as cut and dried but I think Kunta's analogy explained well.  I like to use the metaphor that the dikaryon is like a couple living together, and even making decisions together as both nuclei influence development.  But they don't start making babies until the basidia develops.

Parasexuality is genetic recombination though.  A nuclei from a dikaryon can fuse with a nuclei from a mono, or another dikaryon prior to making spores.  But I don't understand it well enough to explain it well.  There's still two nuclei in the new strain.  But one of the nuclei has recombined with one from the donor strain.  It's more like actual sex (similar to how spores are) than the initial hooking up into dikaryons.

Quote:

tultedspoon said:
I can't count how many times I've specified that I have been doing BRF PF tek

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show one successful brf pf tek jar, one proof that you followed the TEK.

chris77 said:

so…. lets spin the wheel..
am i about to get bitchslapped by trich for probably improper pasteurisation (might have done 1 hr instead of 2)
or does this look lile pan cyan (naolinco) ?
any takers?

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Kunta Kente said:

Generalized: Think a deck of cards, each with random cards to total 52.

Haploid = 52 cards, full random deck.
Monokaryote = that haploid nuclei in a cell, looking for another monokaryote
Dikaryote = two decks (haploid nuclei) separated in one cell: n+n : unique 52 cards + unique 52 cards.
Diploid (before sporulation): The two unique decks temporarily mix, forming a 104 card structure. (2n)
Meiosis (sporulation): the huge deck duplicates into a temporary 208 cards structure that then shuffles, which then split, and split again, forming the final 4 decks of unique (compared to parents) 52 cards, haploid cells.

That's the general part of it. The Bipolar or Tetrapolar is "which monokaryotes are allowed to dikaryote together" by having certain markers. Bipolar each haploid has a single marker, tetrapolar each haploid has two markers.

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chris77 said:

chris77 said:

so…. lets spin the wheel..
am i about to get bitchslapped by trich for probably improper pasteurisation (might have done 1 hr instead of 2)
or does this look lile pan cyan (naolinco) ?
any takers?

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do they like to be lightly misted until pins ? tiny beads? or rather hold back and keep rh up?
tx

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Kloakk said:

My easiest substrate that gave good results was 70/30, Coir/Cow Manure Compost and done.
The one I use is:

Substrate recipe:
- 35% Coir
- 30% Cow Manure Compost
- 30% Straw
- 5% Rice Hulls

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