KlarnEvil9 said:

brominegeek93 said:

Which vendor did you buy the cultures from?
I haven't given up. I'm about to buy some agar agar. The one remaining plate seems quite healthy with rhizomes clearly visible.

The Tek uses these small "gladware" containers that aren't available in europe sadly.
Does this work without tape?
https://www.shroomery.org/forums/showflat.php/Number/19208976

Any experience with these chinese jars you can find on ebay?

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Not sure if those are PP5, but check out this thread featuring "holy grail" plastic containers for agar. Slightly less clear than glass, but they make agar much easier for many of us. Might need to look at more recent posts there to find a link to buy them, the earlier ones sold out on the 'zon.

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Also those are pretty  small.

  You can get 50 them on Amazon for $20.
These are 2.8 in diameter and so about 71 mm. So a little bit bigger than a 60 mm plate

https://a.co/d/0foyFwzZ

BeefExtreme said:

BeefExtreme said:

tultedspoon said:

I know a lot of people are using agar for their grows. To achieve reliable flushes and good grows, are most of the epic grow photos on here from a cloned fruit using agar?

Is anyone getting good flushes and grows from spores on agar? Or does all the agar work and transfers somehow achieve mycelium that is more ready to fruit? Is this why agar is recommended moreso than just for clean and non-contaminated samples?

Like maybe doing a couple transfers, that small amount of mycelium hits the growing time needed for it to be more ready to switch to fruiting conditions, then if you spread that agar to some jars or grows, you will end up with way more surface area of mycelium that is ready to fruit? Does this make sense or am I wrong?

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this isnt at all a qq.  theres entire threads about it, namely the ones i linked in the hhg but ill see if i can give you a qa.

depends on the spores and how stable the parent culture was. 

heres an analogy:


lets say i grow a tub from a spore syringe.  that syringe might have more genetic differentiation in it than the country of china.  maybe you get lucky and we see some dense citys.  some skyscrapers.  maybe some apartment complexes.

more often youre going to see some rural farming communities. 

but then we clone from one of those dense cities and put it on a plate.  now you have the entire genetic sequence of the city of beijing.  yes, very dense, but every city has its suburbs and even ghettos of shanty shacks and houses.

so now we clone only the dense clusters of skyscrapers.  okay, now we have culture with a high propensity to fruit. 

keep doing this and eventually youll get a culture that just wants to make pure sky scrapers.

does that make sense?

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sorry to do this, but i took a lot ot my personal time to answer this question without an acknowledgement from tulted.  just want to make sure he sees it..

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This is one of my favorite analogies I've ever seen you use. You hit it on the head.

Mr Piggy said:

BeefExtreme said:

am I missing something? I don't see the problem with just using the 15 psi setting and running it for the normal amount of time.

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They keep asking the same questions over and over without reading or accepting any of the answers.  Best of luck getting through.  Tulted light.

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Phaethon said:

FounderZ said:

inoculated with live culture 01/29/2026, it's currently on day 14,
What do you guys who grow Lion's Mane think?

I'm assuming this is a fast 10-14 day strain and I can now throw it to my substrate blocks I made.

I'm thinking 2 1/2 cups of spawn (a jar worth) to each 5LB block. Toss a grain on agar to maintain the culture and make liquid culture soon.

Any tips for loading the grow bags, and how to leave them and what to watch for next!?

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You'd get more eyes of lion's mane growers in the gourmet forum. There's a "general discussion" thread if you want to hop on an existing one.

Haven't grown it in a while, but it looks ready to my eyes. Too much longer and it'll start making primordia in the jar.

If the bags are supplemented, you don't need nearly a full jar of grain spawn per bag. Maybe 1/4 of one, even less will be fine for 5 lb of substrate. Thermogenesis (self-heating) can be a problem with high spawn ratios in supplemented bags. If they're pure wood, a full jar can help replace the nutrients you'd otherwise get from supplementation.

The mycelium will be almost invisible on wood too. You have to pay pretty close attention to catch it fully colonized before it starts fruiting in the bag. (Or you can just use that as the signal that it's time to fruit them. ) Are you going to fruit in a tent? Lion's mane likes a ton of FAE (oyster levels) and is also pretty intolerant to low humidity.

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brominegeek93 said:

Kinda unsettles me a bit to boil mushroom substrate in plastic bags - especially with gourmet mushrooms.

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markh said:

I am going to try G2G for the first time and am wondering what the proper procedure is. I will be using my LFH but looking for advice as to how to proceed.

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FounderZ said:

markh said:

I am going to try G2G for the first time and am wondering what the proper procedure is. I will be using my LFH but looking for advice as to how to proceed.

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Just send it, think of it this way 1ML of live culture can knock up an entire jar, that usually looks like a splash of mycelium, throw in what feels like that in sterile environment SAB/Flowhood. It's only wrong if your jars mold out so keep a copy if you need to of the culture.

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